Protective Effect of the Exogenous Hydrogen Sulfide Donor, Parkia speciosa Hassk. against Hypertensive Nephropathy
Main Article Content
Keywords
Parkia speciosa Hassk, hydrogen sulfide, isoproterenol, hypertensive nephropathy, renal protection, western blotting
Abstract
The plant Parkia speciosa Hassk. (PsH) belongs to the family Fabaceae, and it is endemic to Southeast Asia. Its seeds have been used traditionally to treat various ailments, and several investigations have been undertaken to assess its the pharmacological activity. The aim was to ascertain whether PsH, as a hydrogen sulfide donor, could prevent hypertensive nephropathy. We employed a combined in-vitro and in-vivo approach to evaluate the effect of the plant oil on isoproterenol (ISO)-induced cardiac failure in rats. The oil was prepared by hydrodistillation and the antioxidant potential was evaluated by a reducing power assay. Serum kidney biochemical parameters (serum creatinine, urea, uric acid, blood urea nitrogen), serum electrolytes (serum sodium and potassium), and antioxidant biomarkers (malondialdehyde, superoxide dismutase and, glutathione) were measured while (cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) were examined in kidney tissue by western blot. Dissected kidney sections were also examined histopathologically with eosin-hematoxylin staining and microscopy. The plant oil possessed a maximum reducing power at a concentration of 100µM. Subsequently, in-vivo findings revealed that the oil reversed ISO modified metabolic data, serum kidney biochemical parameters, serum electrolytes, and antioxidant biomarkers in renal tissues. The oil also reversed the ISO-inhibited endogenous H2S generating enzymes, CBS and CSE as disclosed by western blot. Additionally, all findings were supported by tissue histopathology. Arising from the study outcomes, PsH oil may have value as an H2S donating nutrapharmaceutical in reducing biochemical aspects of hypertensive nephropathy.
References
2. Hart PD, Bakris GL. Hypertensive nephropathy: prevention and treatment recommendations. Expert opinion on pharmacotherapy. 2010;11(16):2675-86.
3. Dong Z, Dai H, Feng Z, Liu W, Gao Y, Liu F, et al. Mechanism of herbal medicine on hypertensive nephropathy. Molecular Medicine Reports. 2021;23(4):1-.
4. Hsu C-N, Tain Y-L. Hydrogen sulfide in hypertension and kidney disease of developmental origins. International journal of molecular sciences. 2018;19(5):1438.
5. Feliers D, Lee HJ, Kasinath BS. Hydrogen sulfide in renal physiology and disease. Antioxidants & redox signaling. 2016;25(13):720-31.
6. Ried K, Fakler P. Potential of garlic (Allium sativum) in lowering high blood pressure: mechanisms of action and clinical relevance. Integrated blood pressure control. 2014:71-82.
7. Liang D, Wang C, Tocmo R, Wu H, Deng L-W, Huang D. Hydrogen sulphide (H2S) releasing capacity of essential oils isolated from organosulphur rich fruits and vegetables. journal of functional foods. 2015;14:634-40.
8. Al Batran R, Al-Bayaty F, Jamil Al-Obaidi MM, Abdualkader AM, Hadi HA, Ali HM, et al. In vivo antioxidant and antiulcer activity of Parkia speciosa ethanolic leaf extract against ethanol-induced gastric ulcer in rats. PloS one. 2013;8(5):e64751.
9. Kamisah Y, Othman F, Qodriyah HMS, Jaarin K. Parkia speciosa hassk.: A potential phytomedicine. Evidence-Based Complementary and Alternative Medicine. 2013;2013.
10. Wonghirundecha S, Benjakul S, Sumpavapol P. Total phenolic content, antioxidant and antimicrobial activities of stink bean (Parkia speciosa Hassk.) pod extracts. Songklanakarin Journal of Science & Technology. 2014;36(3).
11. Jin CB, Noor H. The hypoglycemic effect of aqueous seed extract of Parkia speciosa on rats. J Trop Med Plants. 2008;9(1):39-42.
12. Tandi J, Handayani TW, Tandebia M, Wijaya JA. Effect of parkia speciosa hassk peels extract on total cholesterol levels of hypercholesterolemia rats. Indian J Forensic Med Toxicol. 2020;14:2988-92.
13. Mustafa N-H, Ugusman A, Jalil J, Kamisah Y. Anti-inflammatory property of Parkia speciosa empty pod extract in human umbilical vein endothelial cells. Journal of applied pharmaceutical science. 2018;8(1):152-8.
14. Kamisah Y, Zuhair JSF, Juliana AH, Jaarin K. Parkia speciosa empty pod prevents hypertension and cardiac damage in rats given N (G)-nitro-l-arginine methyl ester. Biomedicine & Pharmacotherapy. 2017;96:291-8.
15. Tocmo R, Liang D, Wang C, Poh J, Huang D. Organosulfide profile and hydrogen sulfide-releasing capacity of stinky bean (Parkia speciosa) oil: Effects of pH and extraction methods. Food chemistry. 2016;190:1123-9.
16. Han F, Ma G-q, Yang M, Yan L, Xiong W, Shu J-c, et al. Chemical composition and antioxidant activities of essential oils from different parts of the oregano. Journal of Zhejiang University Science B. 2017;18(1):79.
17. Mehdi S, Ahmad F-u-D, Lodhi AH, Khurshid U, Khalid AA, Sidiq SS, et al. Protective Effects of p-CA Against Acute Liver Damage Induced by LPS/D-GalN in Wistar Albino Rats. Drug Design, Development and Therapy. 2022:3327-42.
18. Jiang T, Han F, Gao G, Liu M. Mangiferin exert cardioprotective and anti-apoptotic effects in heart failure induced rats. Life sciences. 2020;249:117476.
19. Sohrabi R, Pazgoohan N, Seresht HR, Amin B. Repeated systemic administration of the cinnamon essential oil possesses anti-anxiety and anti-depressant activities in mice. Iranian journal of basic medical sciences. 2017;20(6):708.
20. Cao X, Bian J-S. The role of hydrogen sulfide in renal system. Frontiers in pharmacology. 2016;7:385.
21. Cheong N, Mohamed E, Haron N, Camalxaman S, Abdullah A, MI MY, et al. Phytochemical quantification and HPLC analysis of Parkia speciosa pod extract. The Medical Journal of Malaysia. 2024;79(Suppl 1):34-9.
22. Ghasemzadeh A, Jaafar HZ, Bukhori MFM, Rahmat MH, Rahmat A. Assessment and comparison of phytochemical constituents and biological activities of bitter bean (Parkia speciosa Hassk.) collected from different locations in Malaysia. Chemistry central journal. 2018;12:1-9.
23. Chhikara N, Devi HR, Jaglan S, Sharma P, Gupta P, Panghal A. Bioactive compounds, food applications and health benefits of Parkia speciosa (stinky beans): a review. Agriculture & food security. 2018;7(1):1-9.
24. Guimarães R, Sousa MJ, Ferreira IC. Contribution of essential oils and phenolics to the antioxidant properties of aromatic plants. Industrial Crops and Products. 2010;32(2):152-6.
25. Liu G, Zhu W, Zhang J, Song D, Zhuang L, Ma Q, et al. Antioxidant capacity of phenolic compounds separated from tea seed oil in vitro and in vivo. Food Chemistry. 2022;371:131122.
26. Waz S, Heeba GH, Hassanin SO, Abdel-Latif RG. Nephroprotective effect of exogenous hydrogen sulfide donor against cyclophosphamide-induced toxicity is mediated by Nrf2/HO-1/NF-κB signaling pathway. Life Sciences. 2021;264:118630.
27. Xia W-J, Liu K-L, Wang X-M, Yang Y, Meng T, Qiao J-A, et al. Hypothalamic paraventricular nucleus hydrogen sulfide exerts antihypertensive effects in spontaneously hypertensive rats via the Nrf2 pathway. American Journal of Hypertension. 2023;36(6):306-15.
28. Askari H, Seifi B, Kadkhodaee M, Sanadgol N, Elshiekh M, Ranjbaran M, et al. Protective effects of hydrogen sulfide on chronic kidney disease by reducing oxidative stress, inflammation and apoptosis. EXCLI journal. 2018;17:14.
29. Liang D, Bian J, Deng L-W, Huang D. Cyclic polysulphide 1, 2, 4-trithiolane from stinky bean (Parkia speciosa seeds) is a slow releasing hydrogen sulphide (H2S) donor. Journal of Functional Foods. 2017;35:197-204.
30. Wang Y, Xing Q-Q, Tu J-K, Tang W-B, Yuan X-N, Xie Y-Y, et al. Involvement of hydrogen sulfide in the progression of renal fibrosis. Chinese medical journal. 2019;132(23):2872-80.
31. Han SJ, Kim JI, Park J-W, Park KM. Hydrogen sulfide accelerates the recovery of kidney tubules after renal ischemia/reperfusion injury. Nephrology Dialysis Transplantation. 2015;30(9):1497-506.
Article Sidebar
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
Ammara Asif
Department of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Fiaz-ud-Din Ahmad
Professor Department of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Osman Asghar Mirza
Assistant Professor Department of Drug Design and Pharmacology, University of Copenhagen, Denmark.
Ruyu Yan
Ph.D student Department of Drug Design and Pharmacology, University of Copenhagen, Denmark.
Robert D. E. Sewell
ProfessorDepartment of Pharmacy, CECOS University, Peshawar, 25000, Khyber Pakhtunkhwa, Pakistan.