Extraction, phytochemical potential and in vitro biological activities of Amygdalus brahuica roots
Main Article Content
Keywords
Amygdalus brahuica, DPPH, phytochemicals, MTT assay, antibacterial, anti-inflammatory
Abstract
The major aim of this research work was to explore the phytochemical and biological potential of Amygdalus brahuica, a traditional medicinal plant which has not been extensively investigated in literature archives. The bioactive compounds from roots of this plant were extracted by using methanol and further fractionated into methanol, dimethyl sulfoxide (DMSO), ethyl acetate and hexane. The antioxidant potential was determined by DPPH and H2O2 scavenging assays while anti-inflammatory response was evaluated through protein denaturation method. The cytotoxic potential of these fractions was evaluated by using HeLa cervical cancer cell line. Antibacterial potential of various fractions was measured by zone of inhibition developed against exposure to different gram +ve and -ve bacterial strains. HPLC analysis revealed that methanolic fraction possess maximum concentration of p-coumaric acid (200.10 µg/g) along with other four phenolic compounds. Moreover, results of total phenolic content showed maximum concentration in methanolic fraction (56.8±0.79 mg GAE/g) while maximum total flavonoid contents in DMSO fraction (46.16±0.74 mg QE/g). Maximum DPPH scavenging potential with lowest IC50 (17.01 µg/mL) was showed by methanolic fraction while ethyl acetate fraction showed maximum H2O2 inhibition with lowest IC50 (13.71 µg/mL) as compared to all other fractions. Moreover, maximum anti-inflammatory response was observed in ethyl acetate fraction (79.29 ± 0.30%). The ethyl acetate fraction exhibited maximum antibacterial potential as compared to other fractions while, methanolic fraction showed maximum antiproliferative potential 44.4% against HeLa cells.
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Adil Mustafa
Department of Chemistry, Government College University Faisalabad-38000, Pakistan
Tanveer Hussain Bokhari
Department of Chemistry, Government College University Faisalabad-38000, Pakistan
Yasir Iqbal
Department of Chemistry, Government College University Faisalabad-38000, Pakistan ; Department of Chemistry, University of Sialkot-51040, Pakistan
Shahzad Ali Shahid Chatha
Department of Chemistry, Government College University Faisalabad-38000, Pakistan
Abdullah Ijaz Hussain
Department of Chemistry, Government College University Faisalabad-38000, Pakistan