VIRULENCE FACTORS AND ANTIBIOTIC RESISTANCE IN STAPHYLOCOCCUS AUREUS: A COMPREHENSIVE STUDY
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Keywords
Antibiotics Susceptibility, Antibiotics Resistance, Antimicrobial stewardship, Staph. aureus, Vancomycin, Coagulase and Gram Staining
Abstract
Objectives
- To identify antibiotics susceptibility pattern of Staph. aureus, focusing on the virulence factors contributing to its pathogenicity.
- To assess the impact of antibiotic misuse on the emergence of antibiotic-resistant strains.
Study Design: Prospective cross-sectional design.
Study Setting: Institute of Medical Lab Technology Isra University Islamabad Campus in collaboration with Microbiology Department of Al Nafees Medical Collage & Hospital (ANMCH), Islamabad.
Study Duration: September 25th 2023 to January 31st, 2024.
Sample Size: Puss swabs were taken from 100 patients which were considered as positive for staph. aureus.
Methodology: Written informed consent was taken from patient visiting microbiology lab of Al-Nafees Hospital. Pus swab samples were collected from 100 individuals, and microbiological techniques were meticulously employed for the isolation and identification of Staph. Aureus strains. The assessment included the examination of key virulence factors such as coagulase, catalase, and toxins. Antibiotic susceptibility testing was conducted to determine resistance levels.
Results: The antibiotic susceptibility testing revealed varying degrees of resistance among the Staph. aureus strains. Notably, vancomycin and linezolid demonstrated the highest efficacy, while penicillin and amoxicillin exhibited lower effectiveness.
Conclusion: The research contributes valuable insights to the understanding of antibiotic resistance patterns, emphasizing the necessity for collaborative efforts in antimicrobial stewardship.
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Ammar Mehfooz
Department of Medical Lab Technology, Al Nafees Medical College and Hospital, Isra University Islamabad, Pakistan
Maryam Almas
Department of Medical Lab Technology, Al Nafees Medical College and Hospital, Isra University Islamabad, Pakistan
Maria Mukhtar
Department of Medical Lab Technology, Al Nafees Medical College and Hospital, Isra University Islamabad, Pakistan
Afsar Ali
Department of Medical Lab Technology, Al Nafees Medical College and Hospital, Isra University Islamabad, Pakistan
Iqra Arooj
Institute of Industrial Biotechnology, IIB, Government College University, Lahore Pakistan
Maryam Khalid
Department of Medical Lab Technology, Al Nafees Medical College and Hospital, Isra University Islamabad, Pakistan
Shahzeera Begum
Department of Medical Lab Technology, Al Nafees Medical College and Hospital, Isra University Islamabad, Pakistan
Rehmatullah Zadran
WHO-surveillance Department, National Infectious Disease Laboratory, Kabul, Afghanistan
Mohsan Aslam
Department of Medical Lab Technology, Al Nafees Medical College and Hospital, Isra University Islamabad, Pakistan
Mehak Khalid
MBBS, MCPS-Gynaecologist