ANALYTICAL TECHNIQUES IN NEUROPHARMACOLOGY: SIMULTANEOUS DETERMINATION OF CITICOLINE SODIUM AND PIRACETAM BY UV-SPECTROSCOPY AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
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Keywords
Citicoline Sodium, Piracetam, UV- spectroscopy method, RP-HPLC, Phosphate buffer, ammonium formate buffer, Acetonitrile
Abstract
The aim of this research is to develop a UV spectrophotometric method and HPLC method that can estimate both citicoline sodium and piracetam simultaneously in pharmaceutical formulations. A phosphate buffer at pH 6.8 was chosen as the suitable medium for the analysis in the study. Citicoline sodium was found to have a concentration range of 10 to 80µ g/ml, while piracetam's concentration range was 10-80 µg/ml.
Citicoline sodium and piracetam were UV absorbed using 272nm and 207.28 nm wavelengths, with the iso-absorptive point being 219.8nm as reported in the information. The calibration curves were made and showed a linear relationship between concentration and absorbance for both compounds. Within the specified concentration ranges, the method demonstrated excellent precision and accuracy.
The UV estimation method's stability and reproducibility are improved by using a phosphate buffer at pH 6.8.A reverse phase high performance liquid chromatographic method that is simple, fast, sensitive, and validated was developed to estimate Citicoline and Piracetam simultaneously in pharmaceutical dosage forms.
The chromatographic conditions used for the separation was agilent eclipse XDB(4.6 x 250 mm x 5 μm) with mobile phase comprised of Acetonitrile: Ammonium formate pH-3.0 adjusted with OPA (20: 80).
The flow rate was determined at 1.0 ml/min and 269 nm was detected. The analysis revealed that the retention times for Citicoline and Piracetam were determined to be 5.615 and 2.258, respectively. The methodology was formulated with the aim of attaining precision, accuracy, specificity, linearity, system suitability, and stability assessments, incorporating evaluations for limits of detection, quantification, ruggedness, and robustness.
Citicoline sodium and Piracetam exhibited linearity within the ranges of 12.50 to 75.00 µg/ml and 10 to 60g/ml, respectively. All specified parameters fell within the designated limits. The methodologies proposed are deemed suitable for the concurrent analysis of Citicoline and Piracetam in pharmaceutical formulations
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Kodukula Mohana Anjani Durga
Department of pharmaceutical analysis, Gitam school of pharmacy,GITAM(Deemed to be university ),Rushikonda ,Visakhapatnam ,Andhra Pradesh-530045,INDIA
Asha Deepti Choppala
Department of pharmaceutical analysis, Gitam school of pharmacy,GITAM(Deemed to be university ),Rushikonda ,Visakhapatnam ,Andhra Pradesh-530045,INDIA