COMPARATIVE ANALYSIS OF HISTOLOGICAL AND HEMATOLOGICAL OF CRITICAL-SIZED FEMUR FRACTURES TREATED WITH IM-PIN IN COMBINATION WITH PMMA AND CAP IN A CANINE MODEL
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Keywords
critically sized, canine model, femur fracture, IM-Pin, Histological, hemato-biological
Abstract
This study aimed to assess critical-size bone defect reconstruction using polymethylmethacrylate (PMMA) and calcium phosphate (CaP) bone materials in comparison, focusing on histological, and hematological parameters. Thirty-six healthy dogs were divided into three groups, with each group receiving different treatments: intramedullary pin (IM-pin) in Group A, PMMA with IM-pin in Group B, and CaP-based bone graft material with IM-pin in Group C. Histological evaluations at various post-surgery time points revealed differences in healing responses, trabeculae formation, and osteon numbers among the groups. Group B exhibited significant growth and dense connective tissue formation, indicating a rapid healing process. IM-Pin PMMA has been recognized as the optimal implant material for femoral bone fractures in dogs, exhibiting histological advantages, including biocompatibility, corrosion resistance, and minimal bone resorption. Throughout all groups, serum calcium levels were maintained within the normal range. Hematological parameters, encompassing red blood cell (RBC) count, hemoglobin, packed cell volume (PCV), white blood cell (WBC) count, neutrophils, lymphocytes, and monocytes, were thoroughly assessed. Variations in RBC count and WBC count were observed post-surgery, with eventual normalization. Overall, this study provides valuable insights into the histological and hematological aspects of critical-size bone defect reconstruction, highlighting the effectiveness of IM-Pin PMMA and the need for long-term safety considerations in veterinary practices.
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Abdul Salam Khoso
Department of Surgery and Obstetrics, Sindh Agriculture University Tandojam
Ahmed Nawaz Tunio
Department of Surgery and Obstetrics, Sindh Agriculture University Tandojam
Muhammad Ghiasuddin Shah
Department of Veterinary Anatomy and Histology, Sindh Agriculture University Tandojam
Akeel Ahmed Memon
Department of Animal Breeding and Genetics, Sindh Agriculture University Tandojam
Atique Ahmed Behan
Department of Livestock Management, Sindh Agriculture University Tandojam
Mansoor Tariq
Department of Veterinary Pathology, Sindh Agriculture University Tandojam
Muhammad Bilawal Arain
Department of Veterinary Pharmacology, Sindh Agriculture University Tandojam