ANTIMICROBIAL EFFECTS OF SPIDER SILK INCORPORATION IN GLASS IONOMER CEMENT
Main Article Content
Keywords
Antibacterial activity, Cervical caries, Glass Ionomer Cement, Recurrent Caries, Spider Silk Protein, Streptococcus Mutant
Abstract
Objective: An agar plate diffusion test was used to assess the bactericidal activity of GIC combined with 5, 10, and 15% spider silk protein.
Design of the study: An-in vitro experiment.
Study Setting: Sardar Begum Dental College in Pakistan, Department of Science in Dental Materials.
Methodology: The specimen of experimental groups B, C & D were prepared after mixing GIC with 5, 10 & 15 wt. % spider silk protein. All these groups were evaluated for their antibacterial activities by measuring the minimum Inhibition concentration as well as the formation of the Inhibition zone at respective concentrations by agar plate diffusion test. The collected data were recorded and subjected to a One-way ANOVA test for statistical significance. The statistical significance between the groups was determined using the post-hoc Tukey test. p is less than 0.05.
Results: The spider silk protein's minimal inhibitory concentration was 10 weight percent. Day 1, Day 7, Day 14, and Day 28 mean inhibition zone for control group A and experimental group B was 0.00mm. For experimental groups C and D, the mean inhibition zone at days 1, 7, 14, and 28 ranged from 0.6 mm to 2.7 mm. In comparison to control group A, experimental group B, and experimental group C, glass ionomer cement containing 15 weight percent SSP in experimental group D yields superior antibacterial activity results.
Conclusion: The Addition of spider silk protein in 10 & 15 wt. % increased the antibacterial activity against S. mutans.
References
2. McCabe JF, Yan Z, Al Naimi O, Mahmoud G, Rolland S. Smart materials in dentistry. Australian dental journal. 2011;56:3-10.
3. Kampanas N-S, Antoniadou M. Glass ionomer cements for the restoration of non-carious cervical lesions in the geriatric patient. Journal of functional biomaterials. 2018;9(3):42.
4. Sharafeddin F, Feizi N. Evaluation of the effect of adding micro-hydroxyapatite and nano-hydroxyapatite on the microleakage of conventional and resin-modified Glass-ionomer Cl V restorations. Journal of Clinical and Experimental Dentistry. 2017;9(2):e242.
5. Santiago SL, Passos VF, Vieira AHM, Navarro MFdL, Lauris JRP, Franco EB. Two-year clinical evaluation of resinous restorative systems in non-carious cervical lesions. Brazilian dental journal. 2010;21:229-34.
6. Hamada S, Slade HD. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiological reviews. 1980;44(2):331-84.
7. Wang S-P, Ge Y, Zhou X-D, Xu HH, Weir MD, Zhang K-K, et al. Effect of anti-biofilm glass–ionomer cement on Streptococcus mutans biofilms. International journal of oral science. 2016;8(2):76-83.
8. Marsh PD. Dental plaque as a biofilm and a microbial community–implications for health and disease. BMC Oral health. 2006;6(Suppl 1):S14.
9. Lohbauer U. Dental glass ionomer cements as permanent filling materials?—Properties, limitations future trends. Materials. 2009;3(1):76-96.
10. Phillips R, Isler S. Dental amalgam: An update. The Compendium of continuing education in dentistry. 1983;4(5):397-402.
11. Manhart J, Kunzelmann KH, Chen HY, Hickel R. Mechanical properties of new composite restorative materials. Journal of Biomedical Materials Research: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials. 2000;53(4):353-61.
12. Prabhakar A, Maganti R, Mythri P, Naik SV. A traditional way to combat against Streptococcus mutans. INTERNATIONAL JOURNAL OF AYURVEDIC MEDICINE. 2016;7(1):37-43.
13. Yesilyurt C, Er K, Tasdemir T, Buruk K, Celik D. Antibacterial activity and physical properties of glass-ionomer cements containing antibiotics. Operative dentistry. 2009;34(1):18-23.
14. TÜRKÜN LSE, Türkün M, ERTUG˘ RUL F, Ates M, Brugger S. Long‐term antibacterial effects and physical properties of a chlorhexidine‐containing glass ionomer cement. Journal of Esthetic and Restorative Dentistry. 2008;20(1):29-44.
15. Al-Kalifawi EJ, Kadem YJ. The antimicrobial activity of Al-Ankabut’s home (Spider’s web) extract. Mesopotamia Envir J. 2017;3(1):54-63.
16. Chung H, Kim TY, Lee SY. Recent advances in production of recombinant spider silk proteins. Current opinion in biotechnology. 2012;23(6):957-64.
17. Cai Y, Guo J, Chen C, Yao C, Chung S-M, Yao J, et al. Silk fibroin membrane used for guided bone tissue regeneration. Materials Science and Engineering: C. 2017;70:148-54.
18. Council on Dental Materials I, Equipment. ANSI/ADA specification no. 66* for dental glass ionomer cements. The Journal of the American Dental Association. 1989;119(1):205.
19. Panpisut P, Monmaturapoj N, Srion A, Toneluck A, Phantumvanit P. Physical properties of glass ionomer cement containing pre-reacted spherical glass fillers. Brazilian Dental Journal. 2020;31:445-52.
20. Prentice LH, Tyas MJ, Burrow MF. The effect of particle size distribution on an experimental glass-ionomer cement. Dental materials. 2005;21(6):505-10.
21. Pitel ML. An improved glass ionomer restorative system: Stress-bearing Class I and II indications. Dentistry today. 2017;36(2):130-4.
22. Rolim FG, de Araújo Lima AD, Lima Campos IC, de Sousa Ferreira R, da Cunha Oliveira-Júnior C, Gomes Prado VL, et al. Fluoride release of fresh and aged glass ionomer cements after recharging with high-fluoride dentifrice. International Journal of Dentistry. 2019;2019.
23. Bellis CA, Addison O, Nobbs AH, Duckworth PF, Holder JA, Barbour ME. Glass ionomer cements with milled, dry chlorhexidine hexametaphosphate filler particles to provide long-term antimicrobial properties with recharge capacity. Dental Materials. 2018;34(12):1717-26.
24. Mutluay AT, Mutluay M. Effects of different disinfection methods on microleakage of giomer restorations. European Journal of Dentistry. 2019;13(04):569-73.
25. Sekhar A, Anil A, Thomas MS, Ginjupalli K. Effect of various dentin disinfection protocols on the bond strength of resin modified glass ionomer restorative material. Journal of clinical and experimental dentistry. 2017;9(7):e837.
26. Fúcio SB, Paula ABd, Sardi JC, Duque C, Correr-Sobrinho L, Puppin-Rontani RM. Streptococcus mutans biofilm influences on the antimicrobial properties of glass ionomer cements. Brazilian dental journal. 2016;27:681-7.
27. Wassel MO, Khattab MA. Antibacterial activity against Streptococcus mutans and inhibition of bacterial induced enamel demineralization of propolis, miswak, and chitosan nanoparticles based dental varnishes. Journal of advanced research. 2017;8(4):387-92.
28. Arbildo-Vega H, Lamas-Lara C, Cruzado-Oliva F, Rosas-Prado C, Gómez-Fuertes A, Vásquez-Rodrigo H. Comparison of the clinical effect of the adhesive strategies of universal adhesives in the treatment of non-carious cervical lesions. Systematic review and meta-analysis. Journal of Oral Research. 2018;7(5):210-22.
29. Igarashi Y, Yoshida S, Kanazawa E. The prevalence and morphological types of non-carious cervical lesions (NCCL) in a contemporary sample of people. Odontology. 2017;105:443-52.
30. Bezerra IM, Brito ACM, de Sousa SA, Santiago BM, Cavalcanti YW, de Almeida LdFD. Glass ionomer cements compared with composite resin in restoration of noncarious cervical lesions: A systematic review and meta-analysis. Heliyon. 2020;6(5).
31. Gomes SC, Leonor IB, Mano JF, Reis RL, Kaplan DL. Antimicrobial functionalized genetically engineered spider silk. Biomaterials. 2011;32(18):4255-66.
32. Barroso H, Ramalhete R, Domingues A, Maci S. Inhibitory activity of a green and black tea blend on Streptococcus mutans. Journal of oral microbiology. 2018;10(1):1481322.
33. Jenkins SG, Schuetz AN, editors. Current concepts in laboratory testing to guide antimicrobial therapy. Mayo Clinic Proceedings; 2012: Elsevier.
34. Wright S, Goodacre SL. Evidence for antimicrobial activity associated with common house spider silk. BMC research notes. 2012;5:1-6.
35. Nilebäck L. Recombinant spider silk with antimicrobial properties. 2013.
Article Sidebar
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
Zunaira Shaukat
Department Of Dental Materials, Multan Medical And Dental College, Multan.
Tahir Ali Khan
Department Of Dental Materials, Sardar Begum Dental College And Hospital, Gandhara University, Peshawar.
Munazzah Ejaz
Department Of Dental Materials, Sardar Begum Dental College And Hospital, Gandhara University, Peshawar.
Sahubzada Ammar Ahmad
Department Of Dental Materials, Khyber College Of Dentistry, Peshawar.
Bilal Zaman Babar
Department Of Dental Materials, Women Medical And Dental College
Aiman Khan
Department Of Dental Materials, Khyber College Of Dentistry, Peshawar