PHARMACOLOGICAL INVESTIGATION IN ANTICANCER AND ANTIOXIDANT PROPERTY OF CHRYSANTHEMUM MORIFOLIUM RAMAT.
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Keywords
Chrysanthemum morifolium Ramat, angiogenesis, medicinal plants, anticancer and anti-oxidants
Abstract
Chrysanthemum morifolium Ramat., commonly referred to as the decorative chrysanthemum or simply mum, has served ornamental and therapeutic objectives historically. Traditionally employed to assuage inflammation, fever, and hypertension, modern exploration has paid particular focus to its conceivable function as an antineoplastic and antioxidant ingredient. Its bioactive mixtures, incorporating flavonoids and polyphenols, have proven potential in discouraging malignant cell proliferation, stimulating apoptosis, and bettering antioxidant safeguards.Various tactics were utilized in the research to investigate CM's pharmacological impacts. Plant extracts were ready using methanol, and their cytotoxicity was evaluated using cell viability assays (MTT, Crystal Violet, Trypan Blue). IC50 values were calculated for HeLa and HepG2 cancerous cell lines. Moreover, antioxidant properties were assessed using catalase (CAT), ascorbate peroxidase (APOX), and glutathione reductase (GSH) assays. Enzyme-linked immunosorbent assays (ELISA) measured fluctuations in p53 and VEGF levels. CM revealed dose-dependently cytotoxic impacts against HeLa and HepG2 cells, with notably reductions in cell viability and heightened cell death seen at elevated concentrations. IC50 values indicated powerful antineoplastic action while sparing noncancerous 3T3 cells. Antioxidant assays uncovered enhanced CAT, APOX, and GSH activities in cancerous cells treated with CM, proposing a role in oxidative stress supervision.Chrysanthemum morifolium Ramat. puts forth encouraging anticancer properties through selective cytotoxicity and apoptotic induction in cancerous cells, backed by heightened antioxidant defenses. Its safety profile in normal cells emphasizes its potential as a therapeutic agent with diminished side effects. However, further exploration is necessary to elucidate the underlying molecular mechanisms and validate its efficacy in animal models and clinical trials. These discoveries underscore CM as a candidate for developing targeted cancer therapies and enhancing overall human health.
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Maliha Munawar
Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Pakistan.
Sana Javaid Awan
Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Pakistan.
Alina Farooq
Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Pakistan.
Mubeen Shahzadi
Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Pakistan.
Bushra Adeel
Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Pakistan.
Haleema Usman Khan
Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Pakistan.