IDENTIFICATION AND QUANTIFICATION OF FLAVONOIDS IN CARICA PAPAYA STEM :HEPATOPROTECTIVE AND ANTIOXIDANT ACTIVITIES
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Keywords
Carica papaya, Flavonoids, Antioxidant, Hepatoprotective
Abstract
Carica papaya, commonly known as papaya, is a tropical fruit-bearing plant recognised for its nutritional and medicinal properties. The stem of this plant, rich in bioactive flavonoids, has shown potential therapeutic benefits, particularly in liver protection and antioxidant activity. This study aimed to identify and quantify the flavonoid content in Carica papaya stems and investigate their hepatoprotective and antioxidant activities. Methanol extraction yielded 25.6 grams of crude extract from 100 grams of powdered stem material, with a purification yield of 22.66% for flavonoids. Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analyses confirmed the presence and quantification of quercetin, kaempferol, and rutin, with quercetin being the most abundant. The purified flavonoid extract exhibited significant peroxynitrite-scavenging activity, demonstrating strong antioxidant properties. These findings underscore the therapeutic potential of Carica papaya stem flavonoids in protecting against oxidative stress and liver damage, supporting their use in developing flavonoid-based interventions for liver diseases and related disorders.
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Richa Pandey
School of Sciences, MATS University, Raipur, Chhattisgarh
Bhagyashree Deshpande
School of Sciences, MATS University, Raipur, Chhattisgarh
Saman Siddiqui
Department of Zoology, Bharti Vishwavidyalaya, Durg,Chhattisgarh
Vishwaprapaksh Roy
School of Sciences, MATS University, Raipur, Chhattisgarh