SPECIES IDENTIFICATION AND DIFFERENTIATION OF DERMATOPHYTES, ANTIFUNGAL SUSCEPTIBILITY PROFILE FROM A TERTIARY CARE CENTRE IN CENTRAL INDIA
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Keywords
Dermatophytes, Trichophyton, antifungal susceptibility, Griseofulvin, Terbinafine
Abstract
Objective: One of the oldest disease, dermatophytosis, is growing more common in the modern era. Because of this, understanding antifungal susceptibility has become crucial in the modern era..
Material and Methods: This 18-month prospective study included all dermatophytes that were isolated during that time. Dermatophytes were discovered using standard phenotypic techniques. As per the Clinical Laboratory Standard Institute M38A2, antifungal susceptibility testing was carried out for griseofulvin, terbinafine, and itraconazole, and minimum inhibitory concentrations (MICs) were determined within 5 days.
Results: Information on the patient and related risk factors were noted. Of the 320 patients with dermatophytosis of the skin, 79.3% had fixed dose combination medications with steroids. 303 of the 272 dermatophytes that were isolated throughout the study period came from skin scrapings, and 48 came from nail samples. Presentations of tinea corporis with cruris were the most frequent. Trichophyton mentagrophytes complex (40.3%)was the most often isolated dermatophyte from skin scrapings (129 out of 272), whereas Trichophyton rubrum complex ( 66%) ,( 32 out of 48 ) was recovered from nail samples. Itraconazole had the lowest MICs, followed by terbinafine and griseofulvin, according to the MIC50 and MIC90 data. In summary Antifungal resistance and species distribution epidemiology are evolving, necessitating ongoing monitoring of these dermatophyte characteristics.
References
2. Sharma V., Kumawat T. K., Sharma A., Seth R. and Chandra S. (2015). Dermatophytes: Diagnosis of dermatophytosis and its treatment. African Journal of Microbiology, 9 (19): 1286-93.
3. Poyyamozhi J. S. and Lakshmanan A. (2018). Profile of dermatophyte infections among rural population: A facility based prospective observational study. International Journal of Community Medicine and Public Health, 5: 1354- 1359.
4. Bhatia V. K. and Sharma P. C. (2015). Determination of minimum inhibitory concentrations of itraconazole, terbinafine and ketoconazole against dermatophyte species by broth microdilution method. Indian journal of medical microbiology, 33 (4): 533.
5. Piraccini B. M. and Alessandrini A. (2015). Onychomycosis: a review. Journal of Fungi, 1 (1): 30-43.
6. Vandeputte P., Ferrari S. and Coste A. T. (2011). Antifungal resistance and new strategies to control fungal infections. International Journal of Microbiology: 2012
7. Robert R, Pihet M. Conventional methods for the diagnosis of dermatophytosis. Mycopathologia 2008;166(5-6):295–306
8. Clinical and Laboratory Standards Institute. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Approved Standard Second Edition. CLSI Document M38– A2. Wayne, PA:: CLSI;; 2008
9. Al-Janabi A. H. (2014). Dermatophytosis: causes, clinical features, signs and treatment. Journal of Symptoms and Signs, 3 (3): 200-
10. Kumar S. (2017). Clinico Microbial Identification and Characterization of Dermatophytes and Mrsa in Jaipur.
11. Khade A. S., Burute S. R., Deogude S. S., Jadhav P. and Raman and S. J. A (2018). Study of Clinical Profile of Dermatophytosis with a Changing Clinical Pattern at a Tertiary Care Centre. Journal of Medical Science and Clinical Research, 6 (05):622-670.
12. Verma S. and Madhu R. (2017). The great Indian epidemic of superficial dermatophytosis: An appraisal. Indian Journal of Dermatology, 62 (3): 227.
13. Konda C., Surekha J. K., Jahnavi I., Madhuri D. S. and Nagamani K. (2017). Isolation and Identification of Dermatophytes in a Tertiary Care Hospital. International Journal of Current Microbiology and Applied Sciences, 6 (2): 4088-4101.
14. Doddamani P.V., Harshan K.H., Kanta R.C., Gangane R., Sunil K.B. (2013) People’s Journal of Scientific Research, 6:10-3.
15. Janagond A.B., Rajendrant T., Acharya S., Vithiya G.R.A., Charles J. (2016) National Journal of Laboratory Medicine, 5: MO29-MO32.
16. Hosthota A., Gowda T. and Manikonda R. (2018). Clinical profile and risk factors of dermatophytoses: a hospital based study. International Journal of Research, 4 (4): 508.
17. Singal A. and Khanna D. (2011). Onychomycosis: Diagnosis and management. Indian Journal of Dermatology, Venereology and Leprology, 77 (6): 659.
18. Poojary S, Miskeen A, Bagadia J, Jaiswal S, Uppuluri P. A study of in vitro antifungal susceptibility patterns of dermatophytic fungi at a tertiary care center in Western India. Indian J Dermatol 2019;64 (04):277–284
19. Singh A, Masih A, Khurana A, et al. High terbinafine resistance in Trichophyton interdigitale isolates in Delhi, India harbouring mutations in the squalene epoxidase gene. Mycoses 2018;61 (07):477–484
20. Altınbaş R, Özakkaş F, Barış A, Turan D, Şen S. In vitro susceptibility of seven antifungal agents against dermatophytes isolated in İstanbul. Turk J Med Sci 2018;48(03):615–619
21. Kulkarni SS, Bhakre JB, Damle AS. In vitro susceptibility testing of four antifungal drugs against fungal isolates in onychomycosis. Int J Res Med Sci 2018;6:2774–2780
22. Badali H, Mohammadi R, Mashedi O, de Hoog GS, Meis JF. In vitro susceptibility patterns of clinically important Trichophyton and Epidermophyton species against nine antifungal drugs. Mycoses 2015;58(05):303–307
23. Rudramurthy SM, Shankarnarayan SA, Dogra S, et al. Mutation in the squalene epoxidase gene of Trichophyton interdigitale and Trichophyton rubrum associated with allylamine resistance. Anti- microb Agents Chemother 2018;62(05):e02522–e17
24. Ansari S, Hedayati MT, Zomorodian K, et al. Molecular characteri- zation and invitro antifungal susceptibilityof 316 clinical isolates of dermatophytes in Iran. Mycopathologia 2016;181(1-2):89–95
25. Pathania S, Rudramurthy SM, Narang T, Saikia UN, Dogra S. A prospective study of the epidemiological and clinical patterns of recurrent dermatophytosis at a tertiary care hospital in India. Indian J Dermatol Venereol Leprol 2018;84(06):678–684
26. Budhiraja RK, Sharma S, Sharma S, et al. Antifungal susceptibility pattern of dermatomycosis in a tertiary care hospital of North India. Int J Res Dermatol 2018;4:240–245
27. Adimi P, Hashemi SJ, Mahmoudi M, et al. In-vitro activity of 10 antifungal agents against 320 dermatophyte strains using micro- dilution method in Tehran. Iran J Pharm Res 2013;12(03):537–545
28. Sahni K, Singh S, Dogra S. Newer topical treatments in skin and nail dermatophyte infections. Indian Dermatol Online J 2018;9 (03):149–158
29. Sahoo AK, Mahajan R. Management of tinea corporis, tinea cruris, and tinea pedis: a comprehensive review. Indian Dermatol Online J 2016;7(02):77–86
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Sakshi Vishnoi
PhD Scholar, Dept. of Microbiology, Malwanchal University, Indore, Madhya Pradesh, India
Madhurendra Singh Rajput
Professor, Dept. of Microbiology, Malwanchal University, Indore, Madhya Pradesh, India