IMPACT OF OPTIMAL EXTRACTION ON PHARMACOLOGICAL ATTRIBUTES OF ARTEMISIA ABSINTHIUM LINN FROM PAKISTAN

Abstract

The oxidative stress is the precursor of many diseases including diabetes and obesity. The side effects of synthetic drugs and antioxidants urge to explore plant based sources for novel pharmacological agents and natural antioxidants for stress and disease management. In the current work, various compositions of methanol (20%, 40%, 60%, 80% and pure methanol) were used to optimized the extraction process and consequent analysis for extract yields, DPPH scavenging, total phenolic contents, total flavonoid contents, α-glucosidase and pancreatic lipase inhibitions for Artemisia absinthium Lin. The extraction was assisted with freeze drying and ultrasonication. The findings revealed that 80% methanol yielded comparatively higher extract yields (15.80±0.55gm), total phenolic contents (84.20±1.09 mg GAE/g DE) and total flavonoid contents (49.01±1.20 mg RE/g DE) for Artemisia absinthium Lin. The DPPH scavenging % value of 85.80±1.20 was also observed for 80% methanolic extract.The 80% methanolic extract also exhibited higher α-glucosidase inhibition value of 54.20±1.45% and pancreatic lipase inhibition with value of 73.58±1.24%. The high phenolic and flavonoid contents were responsible for better antioxidant activity and dietary enzyme inhibitions. The Artemisia absinthium Lin was observed as a potential source for obesity and diabetes mellitus type-2 management which may be further investigated for novel antioxidant system and therapeutic agents.