Development Of A Novel Uv Spectrophotometric Method For Estimation Of Moxifloxacin Hcl In Simulated Lacrimal Fluid (Slf) Ph 7.4 And Methanol Ar

Abstract

In this study, we worked on the development and validation of a simple, accurate and precise UV
spectrophotometric method for measuring Moxifloxacin HCl in simulated lacrimal fluid (SLF) pH 7.4
and methanol AR. The drug shows the λmax 285 nm in SLF and 282 nm in Methanol. This method
obeys Beer-Lambert's law over the concentration range of 2µg/ml to 10µg/ml, as absorbance values
for this concentration range were found to be linear. The calibration curves were plotted separately for
this concentration range of Moxifloxacin HCl for both the solvents. The correlation coefficients were
found to be 0.995 and 0.997 for SLF and methanol respectively. In SLF, the limit of detection (LOD)
and limit of quantification (LOQ) were found 0.037μg/ml and 0.1123μg/ml and in methanol, they
were found to be 0.073μg/ml and 0.221μg/ml respectively. The interday and intraday assay was
conducted for both simulated lacrimal fluid and methanol and %RSD was found below 2%. The
method was validated as per ICH Q2 (R1) guidelines for its accuracy, precision and sensitivity. The
developed novel method was found to be less toxic, reproducible, accurate and sensitive for routine
quantitative estimation of Moxifloxacin HCl in laboratory..