PHYTOCOMPONENTS ANALYSIS OF DELONIX REGIA (GULMOHAR) LEAVES EXTRACTS: EXPLORING BIOACTIVE COMPOUNDS AND POTENTIAL HEALTH BENEFITS
Main Article Content
Keywords
Delonix regia, acetone, n-hexane, GC-MS, phytocomponents
Abstract
Background: Phytocomponents investigation of Delonix regia leaves extracts involves the systematic study of the diverse chemical compounds present in the leaves of the D. regia tree. D. regia, commonly known as the Gulmohar, is renowned for its vibrant red-orange flowers and ornamental value. However, beyond its aesthetic appeal, researchers have increasingly turned their attention to the potential health benefits offered by its leaves.
Objective: The objective of this study was phytocomponents analysis of D. regia leaves extracts. This study was aimed to investigate the chemical composition, identification and quantification of the D. regia leaves extracts of n-hexane and acetone.
Methodology: D. regia leaves were procured from the local garden of Karachi University. The plant was identified and authenticated from herbarium of the botany department of Karachi University. Extraction of plant material was achieved through Soxhlet extraction. The gas chromatography techniques were used for fractionation and purification of the plant extract, moreover mass spectrometry technique was used for identification of secondary metabolites.
Results: GC-MS analysis on the D. regia acetone and n-hexane leaf extract was performed, and different range of phytocomponents were identified; distinguish through their relative abundance and peak numbers. In the acetone extracts of D. regia leaves, the major compound was found to be 2-Pentanone, 4-hydroxy-4-methyl- (33.16%). Other components were Phytol (29.15%), Vitamin E (19.79%). On the other hand, in the n-hexane extracts of D. regia leaves di-iso-octyl ester (18.75%), nonacosane (14.61%), lupeol (10.95%), 1, 30-triacontanediol (7.27%), and
phytol (6.47%) were present. This analysis emphasizes the distinct chemical profiles of D. regia leaves under different extraction solvents, highlighting the presence of a wide range of phytocomponents with potential bioactive properties.
Conclusion: This investigation give emphasis to the different chemical compositions of D. regia leaves under different extraction solvents, existence of a wide range of phytocomponents suggests the potential bioactive properties, which could be a factor to its conventional medicinal uses and serve as a source for pharmaceutical and nutraceutical development.
References
2. Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength, and limitation. Med Aromat Plants. 2015;4:196.
3. Pandey A, Tripathi S. Concept of standardization, extraction, and pre-phytochemical screening strategies for herbal drug. J Pharmacogn Phytochem. 2014;2:115–9.
4. Doughari JH. Phytochemicals: Extraction methods, basic structures, and mode of action as potential chemotherapeutic agents, phytochemicals––a global perspective of their role in nutrition and health. In: Venketeshwer R, editor. A Global Perspective of Their Role in Nutrition and Health. InTech; 2012. [Last accessed 2019 Jun. 10]. Available from: www.intechopen.com
5. Rungsung W, Ratha KK, Dutta S, Dixit AK, Hazra J. Secondary metabolites of plants in drugs discovery. World J Pharm Res. 2015;4:604–13.Sofowora A. The present status of knowledge of the plants used in traditional medicine in western Africa: A medical approach and a chemical evaluation. J Ethnopharmacol. 1980;2:109–18. [PubMed].
6. Das K, Tiwari RK, Shrivastava DK. Techniques for evaluation of medicinal plant products as antimicrobial agents: Current methods and future trends. J Med Plants Res. 2010;4:104– 11.
7. Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants. 2017;6:42. [PMC free article] [PubMed].
8. Hait, M., Nemu, S.C., Kashyap, N.K. and Chaturwedi, A.,. Physicochemical and phytochemical exploration on flower of Delonex regia. Journal of Medicinal Plants: 2018, 6(3):15-18.
9. Bhorga, P.H. and Kamle, S., Comparative Phytochemical Investigation and Determination of Total Phenols and Flavonoid Concentration in Leaves and Flowers Extract of D. regia (Boj. Ex. Hook). Journal of Drug Delivery and Therapeutics 2019; 9(4-s):1034-1037.
10. Vala, M., & Maitreya, B. Phytochemical analysis and total tannin content (TTC) of D. regia (Bojer ex. hook) Raf. bark by using different solvents collected from Saurashtra region. IABCD 2022;1(2): 144-148.
11. Shantha Sheela, N. Pharmacognostical, Phytochemical and Pharmacological Studies of D. regia (Boojer. Hook.) Raf (Doctoral dissertation, College of Pharmacy, Madras Medical College, Chennai) Research journal of pharmacognosy and phytochemistry 2016; 8(2): 70- 74
12. Bhokare, P., Khadke, A., Kuchekar, G. and Kulkarni, S., Comparative study of different extraction technique and phytochemical screening of D. regia. J Pharmacognosy Phytochem 2018; 7(4):133-138.
13. Shettima AY, Karumi Y, Sodipo OA, Usman H, Tijjani MA. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of bioactive components of ethyl acetate root extract of Guiera senegalensis JF Gmel. Journal of Applied Pharmaceutical Science. 2013 Mar 28;3(3):146-50.
14. Sudha T, Chidambarampillai S, Mohan VR. GC-MS analysis of bioactive components of aerial parts of Kirganelia reticulata Poir (Euphorbiaceae). Journal of Current Chemical and Pharmaceutical Sciences. 2013;3(2):113-22.
15. Fagbemi KO, Aina DA, Coopoosamy RM, Olajuyigbe OO. Gas chromatography-mass spectrometry chemical profile investigation and biological activities of ethylacetate fraction of Baobab (Adansonia digitata L.) pulp used in the treatment of urinary tract infections. Journal of Medicinal Plants for Economic Development. 2022 Jan 11;6(1):117.
16. Ferdosi MF, Khan IH, Javaid A, Fardosi MF. GC-MS examination of methanolic extract of Cirsium arvense flower. Pak. J. Weed Sci. Res. 2021 Apr 1;27(2):173-80.
17. Pammi N, Bhukya KK, Lunavath RK, Bhukya B. Bioprospecting of palmyra palm (Borassus Flabellifer) nectar: unveiling the probiotic and therapeutic potential of the traditional rural drink. Frontiers in Microbiology. 2021 Jun 28;12:683996.
18. Faridha Begum I, Mohankumar R, Jeevan M, Ramani K. GC–MS analysis of bio-active molecules derived from Paracoccus pantotrophus FMR19 and the antimicrobial activity against bacterial pathogens and MDROs. Indian journal of microbiology. 2016 Dec;56:426-32.
19. Patil K, Singh DM. GC-MS Analysis of fresh water Cylindrospermum sp. PCC518, Cylindrospermum sp. PCC 567 ethanol and hexane extracts. Int. J. Herb. Med. 2022;10:15-25.
20. R. Singh, S. A. Dar and P. Sharma, Antibacterial activity and toxicological evaluation of semi purified hexane extract of Urtica dioica leaves, Res. J. Med. Plant., 2012, 6(2),123- 135.
21. Mehdi MA, Alawi AH, Thabet AZ, Alarabi F, Omar GM, Pradhan V. Analysis of bioactive chemical compounds of leaves extracts from Tamarindus indica using FT-IR and GC-MS spectroscopy. Asian Journal of Research in Biochemistry. 2021 Jan 14;8(1):22-34.
22. Karthik Y, Ishwara Kalyani M, Krishnappa S, Devappa R, Anjali Goud C, Ramakrishna K, Wani MA, Alkafafy M, Hussen Abduljabbar M, Alswat AS, Sayed SM. Antiproliferative activity of antimicrobial peptides and bioactive compounds from themangrove Glutamicibacter mysorens. Frontiers in Microbiology. 2023 Feb 17;14:1096826.
23. Khan S, Kaur H, Jhamta R. Evaluation of antioxidant potential and phytochemical characterization using GC-MS analysis of bioactive compounds of Achillea filipendulina (L.) leaves. Journal of Pharmacognosy and Phytochemistry. 2019;8(3):258-65.
24. Khatua S, Pandey A, Biswas SJ. Phytochemical evaluation and antimicrobial properties of Trichosanthes dioica root extract. Journal of Pharmacognosy and Phytochemistry. 2016;5(5):410-3.
25. Mary, F. P. A., and Giri, S.R.: GC-MS Analysis of bioactive compounds of Achyranthes Aspera. World Journal of Pharmaceutical Research;7(2018) 1045-1056.
26. Mladenovi M, MaškoviP et al. Studies on the antimicrobial activity and chemical composition of the essential oils and alcoholic extracts of Gentiana asclepiadea L., J Med Plant Res, 2011; 5(7): 1164-1174.
27. Mammen D, Daniel M, Sane RT. Seasonal and geographical variations in chemical constituents of Leptadenia reticulata. Int J Pharm Sci Rev Res. 2010;4(2):111-6.
28. Shawer EE, Sabae SZ, El-Gamal AD, Elsaied HE. Characterization of Bioactive Compounds with Antioxidant Activity and Antimicrobial Activity from Freshwater Cyanobacteria. Egyptian Journal of Chemistry. 2022 Sep 1;65(9):723-35.
29. R.C. Ohiri, E.B. Essien, Nutraceutical potentials of Turkey Tail medicinal mushroom, Trametes versicolor (Coriolus versicolor, yun zhi, kawaratake) (Agaricomycetes) from Nigeria, Pakistan J. Biochem. Mol. Biol. 50 (1) (2017) 1–6.
30. Naz R, Roberts TH, Bano A, Nosheen A, Yasmin H, Hassan MN, Keyani R, Ullah S, Khan W, Anwar Z. GC-MS analysis, antimicrobial, antioxidant, antilipoxygenase and cytotoxic activities of Jacaranda mimosifolia methanol leaf extracts and fractions. PLoS One. 2020 Jul 29;15(7):e0236319.
31. Chhabra D, Gupta RK. Fortification of curd using D. regia flower petal extract and estimation of its phytochemical, antibacterial & antioxidant activity. Journal of Pharmacognosy and Phytochemistry. 2015;4(3):299-307.
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Dr. Saeed Ahmed Sheikh
MBBS, M.Phil. PhD Scholar, Assistant Professor, Dept. of Pharmacology and Therapeutics, Fazaia Ruth Pfau Medical College, Karachi-74350, Pakistan
Dr. Asadullah
MBBS, M.Phil. MPH, PhD Scholar, Lecturer, Department of Pharmacology, Shaheed Mohtarma Benazir Bhutto Medical College, Karachi
Dr.Asif Ahmed
Professor, Head of Pharmacology and Therapeutics Department, Baqai Medical College - Baqai Medical University, Karachi.
Dr. Shaikh Nadeem Ahmed
Chairman Department of Pharmacology and Therapeutics, Baqai Medical College - Baqai Medical University, Karachi
Dr. Farhat Jahan
MBBS, MPH, Lecturer, Department of Community Medicine, Shaheed Mohtarma Benazir Bhutto Medical College, Karachi,
Dr Shazia Nawaz
Senior Lecturer, Department of Pharmacology and Therapeutics, Fazaia Ruth Pfau Medical College, Karachi-74350, Pakistan
Dr Shazia Dawood
Assistant Professor, Pharmacy and Allied Health Science, Iqra University North Campus, Karachi, Pakistan,