SIMULTANEOUS QUANTIFICATION OF FLAVONOIDS BY USING RP-HPLC, PHYTOCHEMICAL PROFILING AND CYTOTOXIC ACTIVITY OF HIPPOPHAE RHAMNOIDES BERRIES.
Main Article Content
Keywords
Hippophae, Phytochemicals, HPLC, flavonoids, cytotoxicity
Abstract
Hippophae rhamnoides commonly known as Seabuck thorn is native to Europe and Asia. This plant has been reported for the treatment of pulmonary, gastrointestinal, cardiac and blood disorders. The present study, for the first time, was undertaken to explore the biological and phytochemical profiling of the methanol, ethanol and aqueous extracts of berries of H. rhamnoides . Phytochemical analysis revealed the presence of alkaloids, glycosides, tannins, flavonoids and terpenoids. Proximate analysis i.e. moisture content, pH and ash values were in normal range indicating the stability of crude powder. Elemental analysis indicated the presence of essential mineral nutrients in trace amounts. RP-HPLC based quantification revealed the presence of significant amount of flavonoids ranging from 0.005 - 5.450 µg/ml. The plant exhibited considerable levels of cytotoxicity against BHK-21 cell line with the LC50 values ranging between 75-330 µg/ml, while extracts at concentration of 400 μg/ml showed significant cytotoxicity for Vero cell line.
References
2. Slavin, J.L. and B. Lloyd, Health benefits of fruits and vegetables. Advances in Nutrition: An International Review Journal, 2012. 3(4): p. 506-516.
3. Kumari, A., et al., Antioxidant Activities, Metabolic Profiling, Proximate Analysis, Mineral Nutrient Composition of Salvadora persica Fruit Unravel a Potential Functional Food and a Natural Source of Pharmaceuticals. Frontiers in pharmacology, 2017. 8.
4. Abitogun, A., Nutrional and chemical composition of ripe and unripe Vitex glandifolia. J. Res. Natl. Dev, 2010. 8: p. 1-5.
5. Olayiwola, I., et al., Phytonutrient, antioxidant and mineral composition of some wild fruits in south west Nigeria. Nigerian Food Journal, 2013. 31(2): p. 33-40.
6. Li, T.S. and W. Schroeder, Sea buckthorn (Hippophae rhamnoides L.): a multipurpose plant. HortTechnology, 1996. 6(4): p. 370-380.
7. Zeb, A., Chemical and nutritional constituents of sea buckthorn juice. Pakistan Journal of Nutrition, 2004. 3(2): p. 99-106.
8. Zeb, A., Important therapeutic uses of sea buckthorn (Hippophae): a review. Journal of Biological Sciences, 2004. 4(5): p. 687-693.
9. Patel, C.A., et al., Remedial prospective of Hippophaë rhamnoides Linn.(sea buckthorn). ISRN pharmacology, 2012. 2012.
10. Hseu, Z.-Y., Evaluating heavy metal contents in nine composts using four digestion methods. Bioresource technology, 2004. 95(1): p. 53-59.
11. Sultana, B. and F. Anwar, Flavonols (kaempeferol, quercetin, myricetin) contents of selected fruits, vegetables and medicinal plants. Food Chemistry, 2008. 108(3): p. 879-884.
12. Mattana, C., et al., Evaluation of cytotoxicity and genotoxicity of Acacia aroma leaf extracts. The Scientific World Journal, 2014. 2014.
13. Fautz, R., B. Husein, and C. Hechenberger, Application of the neutral red assay (NR assay) to monolayer cultures of primary hepatocytes: rapid colorimetric viability determination for the unscheduled DNA synthesis test (UDS). Mutation Research/Environmental Mutagenesis and Related Subjects, 1991. 253(2): p. 173-179.
14. Nemudzivhadi, V. and P. Masoko, In vitro assessment of cytotoxicity, antioxidant, and anti-inflammatory activities of Ricinus communis (Euphorbiaceae) leaf extracts. Evidence-Based Complementary and Alternative Medicine, 2014. 2014.
15. Tiwary, B.K., et al., The in vitro cytotoxic activity of ethno-pharmacological important plants of Darjeeling district of West Bengal against different human cancer cell lines. BMC complementary and alternative medicine, 2015. 15(1): p. 22.
16. Dharmananda, S., Allnuts and the uses of tannins in Chinese medicine In., proceedings of institutes for traditional medicine port. 3938, land Oregon. Afr. J. Biotechnol. Kindly provide page number, 2003.
17. Waterman, K.C. and R.C. Adami, Accelerated aging: Prediction of chemical stability of pharmaceuticals. International journal of pharmaceutics, 2005. 293(1): p. 101-125.
18. Abbas, Z.K., et al., Phytochemical, antioxidant and mineral composition of hydroalcoholic extract of chicory (Cichorium intybus L.) leaves. Saudi journal of biological sciences, 2015. 22(3): p. 322-326.
19. Saxena, H.O., et al., Phytochemical screening and elemental analysis in different plant parts of Uraria picta Desv.: A Dashmul species. Journal of Chemical and Pharmaceutical Research, 2014. 6(5): p. 756-760.
20. Broadley, M.R., et al., Zinc in plants. New Phytologist, 2007. 173(4): p. 677-702.
21. Avila, D.S., R.L. Puntel, and M. Aschner, Manganese in health and disease, in Interrelations between essential metal ions and human diseases. 2013, Springer. p. 199-227.
22. Saeed, A. and R. Bashier, Physico-chemical analysis of Ximenia americana L. seed oil and structure elucidation of some chemical constituents of its seed oil and fruit pulp. Journal of Pharmacognosy and Phytotherapy, 2010. 2(4): p. 49-55.
23. Singare, P., Study on mineral content of some Ayurvedic Indian medicinal plants by instrumental neutron activation analysis and AAS techniques. Health Science Journal, 2010.
24. Enyiukwu, D., et al., Significance of characterization of secondary metabolites from extracts of higher plants in plant disease management. Int J Adv Agric Res, 2014. 2: p. 8-28.
25. Reddy, N.S., et al., Phenolic content, antioxidant effect and cytotoxic activity of Leea indica leaves. BMC complementary and alternative medicine, 2012. 12(1): p. 128.
26. Finter, N., Dye uptake methods for assessing viral cytopathogenicity and their application to interferon assays. Journal of General Virology, 1969. 5(3): p. 419-427.
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Sahar Zahid
University college of Pharmacy, University of the Punjab, 54000. LAHORE, Pakistan.
Sadia Chaman
University college of Pharmacy, University of the Punjab, 54000. LAHORE, Pakistan.
Shaista Qamar
Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore, 54000, Pakistan;
Rabia saeed
Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore, 54000, Pakistan;
Fatima Usman
Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore, 54000, Pakistan;
Hamza Imam Dar
Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore, 54000, Pakistan;
Ahmad Asghar
Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore, 54000, Pakistan;
Muhammad Ahad Ali
Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore, 54000, Pakistan;
Ali Abbas
Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore, 54000, Pakistan;
Ali Abbas
Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore, 54000, Pakistan;
Toobah Ashraf
Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore, 54000, Pakistan;