PREVALENCE OF HEPATITIS D VIRUS AND ITS IMPACT ON CLINICAL PARAMETERS IN QUETTA, PAKISTAN
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Abstract
Background and Objectives: Hepatitis delta virus (HDV) is a small, 1.7 Kb RNA virus contained in a protein envelope consisting of hepatitis B surface antigen (HbsAg). Hepatitis delta has a worldwide distribution and is often attributed to cause severe and progressive liver disease. HDV infection in Quetta has been reported previously but no comprehensive study is available. The aim of this study was to get first detailed insight of HDV infections and associated risk factors in infected patients.
Methods: It was a cross sectional study. The study was conducted in Balochistan from 2021-22 All the HbsAg positive patients visiting tertiary care hospitals in Quetta were included in the study. The collected blood samples with elevated liver function tests were screened for the detection of HbsAg anti-HDV and anti-HCV. ELISA and qPCR were employed for confirmation and quantification. The collected data was recorded in MS-excel and the standard descriptive analysis was done by using MS-excel and SPSS V20.
Results: The present study showed that 27 participants were tested positive for HDV PCR out of 138 positive HbsAg PCR patients. HbsAg PCR was found to have a significant association with the gender. Similarly, LFT Alkaline Phosphatase, Heamoglobin Levels and WBC Levels were also found to have a significant association with the gender. It was also noted that LFT bilirubin of 133 participants was found increased, LFT SGPT of 284 participants was found increased, 281 participants had increased levels of LFT Alkaline phosphatase, 276 participants had increased LFT y-GT whereas 130 participants were reported with enlarged liver through ultrasound.
Conclusion: This study concludes that every fifth HbsAg positive patients is vulnerable to the development of hepatitis in Quetta, Pakistan.
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Akhtar Hamid Tariq
Center for Advanced Studies in Vaccinology and Biotechnology (CASVAB), University of Balochistan, Quetta Pakistan
Tauseef M Asmat
Center for Advanced Studies in Vaccinology and Biotechnology (CASVAB), University of Balochistan, Quetta Pakistan
Mohammad Zahid Mustafa
Center for Advanced Studies in Vaccinology and Biotechnology (CASVAB), University of Balochistan, Quetta Pakistan
Shahid Pervaz
Department of Pathalogy, Agha Khan University Hospital Karachi, Pakistan