ASSESSMENT OF SPERM ROTATIONAL DYNAMICS AND MOTILITY BEHAVIOR USING LASER-BASED OPTICAL TRAPPING: IMPLICATIONS FOR MALE FERTILITY POTENTIAL AND ASSISTED REPRODUCTIVE TECHNOLOGY
Main Article Content
Keywords
Laser-Based Optical Trapping, Sperm Rotational Dynamics, Male Fertility, Assisted Reproductive Technology, ellular Physiology
Abstract
Laser-based optical trapping provides non-invasive access to cellular physiological and mechanical properties. The aim of this technique is to study the rotational dynamics and motility behavior of sperm cells in order to assess the male fertility potential. An integrated optical system with near-infrared laser beam was used to measure rotational dynamics of live sperm cells from oligozoospermic and asthenozoospermic cases. As the sperm are optically trapped, their translational motions become rotational motions without adversely affecting them. As compared to controls, infertile men's sperm cells rotate at a significantly slower speed. As part of modern reproductive biology, it may be crucial to distinguish between normal and abnormal sperm cells based on beat frequency. It has been proposed that the application of laser-assisted techniques to biology may be a useful tool for improving assisted reproductive technology by assessing sperm fertilization capacity.
References
1. A. Ashkin, “Acceleration and trapping of particles by radiation pressure,” Physical Review Letters, vol. 24, no. 4, pp. 156–159, 1970.
2. A. Ashkin, J. M. Dziedzic, and T. Yamane, “Optical trapping and manipulation of single cells using infrared laser beams,” Nature, vol. 330, no. 6150, pp. 769–771, 1987.
3. A. Ashkin, “Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime,” Methods in Cell Biology, no. 55, pp. 1–27, 1998.
4. K. Bambardekar, A. K. Dharmadhikari, J. A. Dharmadhikari, D. Mathur, and S. Sharma, “Measuring erythrocyte deformability with fluorescence, fluid forces, and optical trapping,” Journal of Biomedical Optics, vol. 13, no. 6, Article ID 064021, 2008.
5. K. Bambardekar, J. A. Dharmadhikari, A. K. Dharmadhikari et al., “Shape anisotropy induces rotations in optically trapped red blood cells,” Journal of Biomedical Optics, vol. 15, no. 4, Article ID 041504, 2010.
6. A. Ghosh, S. Sinha, J. A. Dharmadhikari et al., “Euler bucklinginduced folding and rotation of red blood cells in an optical trap,” Physical Biology, vol. 3, no. 1, pp. 67–73, 2006.
7. J. S. D’Souza, M. Gudipati, J. A. Dharmadhikari et al., “Flagellagenerated forces reveal gear-type motor in single cells of the green alga, Chlamydomonas reinhardtii,” Biochemical and Biophysical Research Communications, vol. 380, no. 2, pp. 266– 270, 2009.
8. E. Araujo Jr., Y. Tadir, P. Patrizio et al., “Relative force of human epididymal sperm,” Fertility and Sterility, vol. 62, no. 3, pp. 585– 590, 1994.
9. L. Z. Shi, J. M. Nascimento, C. Chandsawangbhuwana, E. L. Botvinick, and M. W. Berns, “An automatic system to study sperm motility and energetics,” Biomedical Microdevices, vol. 10, no. 4, pp. 573–583, 2008.
10. J. L. Nascimento, E. L. Botvinick, L. Z. Shi, B. Durrant, and M. W. Berns, “Analysis of sperm motility using optical tweezers,” Journal of Biomedical Optics, vol. 11, no. 4, Article ID 044001, 2006.
11. N. Hyun, C. Chandsawangbhuwana, Q. Zhu, L. Z. Shi, C. YangWong, and M. W. Berns, “Effects of viscosity on sperm motility studied with optical tweezers,” Journal of Biomedical Optics, vol. 17, no. 2, Article ID 025005, 2012.
12. Z. N. Dantas, E. Araujo Jr., Y. Tadir, M. W. Berns, M. J. Schell, and S. C. Stone, “Effect of freezing on the relative escape force of sperm as measured by a laser optical trap,” Fertility and Sterility, vol. 63, no. 1, pp. 185–188, 1995.
13. P. Patrizio, Y. Liu, G. J. Sonek, M. W. Berns, and Y. Tadir, “Effect of pentoxifylline on the intrinsic swimming forces of human sperm assessed by optical tweezers,” Journal of Andrology, vol. 21, no. 5, pp. 753–756, 2000.
14. J. M. Nascimento, L. Z. Shi, C. Chandsawangbhuwana et al., “Use of laser tweezers to analyze sperm motility and mitochondrial membrane potential,” Journal of Biomedical Optics, vol. 13, no. 1, Article ID 014002, 2008.
15. B. Shao, L. Z. Shi, J. M. Nascimento et al., “High-throughput sorting and analysis of human sperm with a ring-shaped laser trap,” Biomedical Microdevices, vol. 9, no. 3, pp. 361–369, 2007.
16. M. M. Garcia, A. T. Ohta, T. J. Walsh et al., “A noninvasive, motility independent, sperm sorting method and technology to identify and retrieve individual viable nonmotile sperm for intracytoplasmic sperm injection,” Journal of Urology, vol. 184, no. 6, pp. 2466–2472, 2010.
17. Y. Tadir, W. H. Wright, O. Vafa, T. Ord, R. H. Asch, and M. W. Berns, “Micromanipulation of sperm by a laser generated optical trap,” Fertility and Sterility, vol. 52, no. 5, pp. 870–873, 1989.
18. L. Shi, B. Shao, T. Chen, and M. Berns, “Automatic annular laser trapping: a system for high-throughput sperm analysis and sorting,” Journal of Biophotonics, vol. 2, no. 3, pp. 167–177, 2009.
19. L. Z. Shi, J. Nascimento, C. Chandsawangbhuwana, M. W. Berns, and E. L. Botvinick, “Real-time automated tracking and trapping system for sperm,” Microscopy Research and Technique, vol. 69, no. 11, pp. 894–902, 2006.
20. T. Kasai, K. Ogawa, K. Mizuno et al., “Relationship between sperm mitochondrial membrane potential, sperm motility, and fertility potential,” Asian Journal of Andrology, vol. 4, no. 2, pp. 97–103, 2002.
21. J. M. Nascimento, L. Z. Shi, S. Meyers et al., “The use of optical tweezers to study sperm competition and motility in primates,” Journal of the Royal Society Interface, vol. 5, no. 20, pp. 297–302, 2008.
22. K. Konig, L. Svaasand, Y. Liu et al., “Determination of motility ¨ forces of human spermatozoa using an 800 nm optical trap,” Cellular and Molecular Biology (Noisy-le-grand), vol. 42, no. 4, pp. 501–509, 1996.
23. K. Konig, Y. Tadir, P. Patrizio, M. W. Berns, and B. J. Tromberg, ¨ “Effects of ultraviolet exposure and near infrared laser tweezers on human spermatozoa,” Human Reproduction, vol. 11, no. 10, pp. 2162–2164, 1996.
24. S. K. Mohanty, M. Sharma, and P. K. Gupta, “Generation of ROS in cells on exposure to CW and pulsed near-infrared laser tweezers,” Photochemical and Photobiological Sciences, vol. 5, no. 1, pp. 134–139, 2006.
25. A. Mei, E. Botvinick, and M. Berns, “Monitoring sperm mitochondrial respiration response in a laser trap using ratiometric fluorescence,” in Optical Trapping and Optical Micromanipulation II, Proceedings of the SPIE 5930, pp. 1–11, San Diego, Calif, USA, August 2005.
26. S. K. Mohanty, A. Rapp, S. Monajembashi, P. K. Gupta, and K. O. Greulich, “Comet assay measurements of DNA damage in cells by laser microbeams and trapping beams with wavelengths spanning a range of 308 nm to 1064 nm,” Radiation Research, vol. 157, no. 4, pp. 378–385, 2002.
27. F. Martinez-Pastor, A. Johannisson, J. Gil et al., “Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen,” Animal Reproduction Science, vol. 84, no. 1-2, pp. 121– 133, 2004.
2. A. Ashkin, J. M. Dziedzic, and T. Yamane, “Optical trapping and manipulation of single cells using infrared laser beams,” Nature, vol. 330, no. 6150, pp. 769–771, 1987.
3. A. Ashkin, “Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime,” Methods in Cell Biology, no. 55, pp. 1–27, 1998.
4. K. Bambardekar, A. K. Dharmadhikari, J. A. Dharmadhikari, D. Mathur, and S. Sharma, “Measuring erythrocyte deformability with fluorescence, fluid forces, and optical trapping,” Journal of Biomedical Optics, vol. 13, no. 6, Article ID 064021, 2008.
5. K. Bambardekar, J. A. Dharmadhikari, A. K. Dharmadhikari et al., “Shape anisotropy induces rotations in optically trapped red blood cells,” Journal of Biomedical Optics, vol. 15, no. 4, Article ID 041504, 2010.
6. A. Ghosh, S. Sinha, J. A. Dharmadhikari et al., “Euler bucklinginduced folding and rotation of red blood cells in an optical trap,” Physical Biology, vol. 3, no. 1, pp. 67–73, 2006.
7. J. S. D’Souza, M. Gudipati, J. A. Dharmadhikari et al., “Flagellagenerated forces reveal gear-type motor in single cells of the green alga, Chlamydomonas reinhardtii,” Biochemical and Biophysical Research Communications, vol. 380, no. 2, pp. 266– 270, 2009.
8. E. Araujo Jr., Y. Tadir, P. Patrizio et al., “Relative force of human epididymal sperm,” Fertility and Sterility, vol. 62, no. 3, pp. 585– 590, 1994.
9. L. Z. Shi, J. M. Nascimento, C. Chandsawangbhuwana, E. L. Botvinick, and M. W. Berns, “An automatic system to study sperm motility and energetics,” Biomedical Microdevices, vol. 10, no. 4, pp. 573–583, 2008.
10. J. L. Nascimento, E. L. Botvinick, L. Z. Shi, B. Durrant, and M. W. Berns, “Analysis of sperm motility using optical tweezers,” Journal of Biomedical Optics, vol. 11, no. 4, Article ID 044001, 2006.
11. N. Hyun, C. Chandsawangbhuwana, Q. Zhu, L. Z. Shi, C. YangWong, and M. W. Berns, “Effects of viscosity on sperm motility studied with optical tweezers,” Journal of Biomedical Optics, vol. 17, no. 2, Article ID 025005, 2012.
12. Z. N. Dantas, E. Araujo Jr., Y. Tadir, M. W. Berns, M. J. Schell, and S. C. Stone, “Effect of freezing on the relative escape force of sperm as measured by a laser optical trap,” Fertility and Sterility, vol. 63, no. 1, pp. 185–188, 1995.
13. P. Patrizio, Y. Liu, G. J. Sonek, M. W. Berns, and Y. Tadir, “Effect of pentoxifylline on the intrinsic swimming forces of human sperm assessed by optical tweezers,” Journal of Andrology, vol. 21, no. 5, pp. 753–756, 2000.
14. J. M. Nascimento, L. Z. Shi, C. Chandsawangbhuwana et al., “Use of laser tweezers to analyze sperm motility and mitochondrial membrane potential,” Journal of Biomedical Optics, vol. 13, no. 1, Article ID 014002, 2008.
15. B. Shao, L. Z. Shi, J. M. Nascimento et al., “High-throughput sorting and analysis of human sperm with a ring-shaped laser trap,” Biomedical Microdevices, vol. 9, no. 3, pp. 361–369, 2007.
16. M. M. Garcia, A. T. Ohta, T. J. Walsh et al., “A noninvasive, motility independent, sperm sorting method and technology to identify and retrieve individual viable nonmotile sperm for intracytoplasmic sperm injection,” Journal of Urology, vol. 184, no. 6, pp. 2466–2472, 2010.
17. Y. Tadir, W. H. Wright, O. Vafa, T. Ord, R. H. Asch, and M. W. Berns, “Micromanipulation of sperm by a laser generated optical trap,” Fertility and Sterility, vol. 52, no. 5, pp. 870–873, 1989.
18. L. Shi, B. Shao, T. Chen, and M. Berns, “Automatic annular laser trapping: a system for high-throughput sperm analysis and sorting,” Journal of Biophotonics, vol. 2, no. 3, pp. 167–177, 2009.
19. L. Z. Shi, J. Nascimento, C. Chandsawangbhuwana, M. W. Berns, and E. L. Botvinick, “Real-time automated tracking and trapping system for sperm,” Microscopy Research and Technique, vol. 69, no. 11, pp. 894–902, 2006.
20. T. Kasai, K. Ogawa, K. Mizuno et al., “Relationship between sperm mitochondrial membrane potential, sperm motility, and fertility potential,” Asian Journal of Andrology, vol. 4, no. 2, pp. 97–103, 2002.
21. J. M. Nascimento, L. Z. Shi, S. Meyers et al., “The use of optical tweezers to study sperm competition and motility in primates,” Journal of the Royal Society Interface, vol. 5, no. 20, pp. 297–302, 2008.
22. K. Konig, L. Svaasand, Y. Liu et al., “Determination of motility ¨ forces of human spermatozoa using an 800 nm optical trap,” Cellular and Molecular Biology (Noisy-le-grand), vol. 42, no. 4, pp. 501–509, 1996.
23. K. Konig, Y. Tadir, P. Patrizio, M. W. Berns, and B. J. Tromberg, ¨ “Effects of ultraviolet exposure and near infrared laser tweezers on human spermatozoa,” Human Reproduction, vol. 11, no. 10, pp. 2162–2164, 1996.
24. S. K. Mohanty, M. Sharma, and P. K. Gupta, “Generation of ROS in cells on exposure to CW and pulsed near-infrared laser tweezers,” Photochemical and Photobiological Sciences, vol. 5, no. 1, pp. 134–139, 2006.
25. A. Mei, E. Botvinick, and M. Berns, “Monitoring sperm mitochondrial respiration response in a laser trap using ratiometric fluorescence,” in Optical Trapping and Optical Micromanipulation II, Proceedings of the SPIE 5930, pp. 1–11, San Diego, Calif, USA, August 2005.
26. S. K. Mohanty, A. Rapp, S. Monajembashi, P. K. Gupta, and K. O. Greulich, “Comet assay measurements of DNA damage in cells by laser microbeams and trapping beams with wavelengths spanning a range of 308 nm to 1064 nm,” Radiation Research, vol. 157, no. 4, pp. 378–385, 2002.
27. F. Martinez-Pastor, A. Johannisson, J. Gil et al., “Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen,” Animal Reproduction Science, vol. 84, no. 1-2, pp. 121– 133, 2004.
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Oct 11, 2023
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Dr. Bhanu Rekha. (2023). ASSESSMENT OF SPERM ROTATIONAL DYNAMICS AND MOTILITY BEHAVIOR USING LASER-BASED OPTICAL TRAPPING: IMPLICATIONS FOR MALE FERTILITY POTENTIAL AND ASSISTED REPRODUCTIVE TECHNOLOGY. Journal of Population Therapeutics and Clinical Pharmacology, 30(6), 587-591. https://doi.org/10.53555/jptcp.v30i6.6052
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Author Biography
Dr. Bhanu Rekha
Assistant Professor, Department of Physiology, Sri Lakshmi Narayana Institute of Medical Sciences & Hospital,Osudu, Puducherry - 605502
How to Cite
Dr. Bhanu Rekha. (2023). ASSESSMENT OF SPERM ROTATIONAL DYNAMICS AND MOTILITY BEHAVIOR USING LASER-BASED OPTICAL TRAPPING: IMPLICATIONS FOR MALE FERTILITY POTENTIAL AND ASSISTED REPRODUCTIVE TECHNOLOGY. Journal of Population Therapeutics and Clinical Pharmacology, 30(6), 587-591. https://doi.org/10.53555/jptcp.v30i6.6052