TB culture,PCR ,Lipoarabinomannan in diagnosis of tuberculosis
Main Article Content
Keywords
Tuberculosis, Methods of TB culture,PCR ,Lipoarabinomannan
Abstract
Tuberculosis (TB) has been a major health problem in developing countries.
Rapid diagnosis of Mycobacterium tuberculosis infection plays a critical role in controlling the spread of tuberculosis. Conventional methods may take up to several weeks or longer to produce results. This study aimed to compare of different methods like culture on bio F-M media,MP BacT/ALERT bottles, and urine lipoarabinomannan by ELISA
Subjects and methods:
This study was done on 100 patients suspected to have tuberculosis, cases divided into two groups :( Group A): 68 clinically suspected pulmonary TB cases. (Group B): 32 clinically suspected extrapulmonary TB cases. All samples from group A and B were subjected to direct staining by Zeihl-Neelsen stain, Culture of the samples on Lowenstein Jensen media, Bio FM media and automated MP BacT/ALERT bottles and detection of urine lipoarabinomannan by ELISA.
Results:
in suspected pulmonary tuberculosis cases the Sensitivity and Specificity of Bio FM were 87.2%, 100% respectively, Sensitivity and Specificity of MP BacT/ ALERT were 74.4%, 100% respectively, Sensitivity and Specificity of urine lipoarabinomannan were 20.5%, 96.9% respectively and in suspected extrapulmonary tuberculosis cases were the Sensitivity and Specificity of Bio FM were 87.5%, 100% respectively, Sensitivity and Specificity of MP BacT/ ALERT were were 50 %, 100% respectively, Sensitivity and Specificity of urine lipoarabinomannan were 12.5%, 100% respectively.
Conclusion:
-The sensitivity of both solid (Lowenstein Jensen) and liquid (Bio FM) media were the same and better than MP BacT/ALERT .Bio FM media and MP BacT/ALERT show shorter time than Lowenstein Jensen for detection of mycobacterial growth .Detection of urine lipoarabinomannan by ELISA is insensitive for the diagnosis of TB,
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