DEVELOPMENT AND OPTIMIZATION OF HPLC ANALYSIS OF METRONIDAZOLE
Main Article Content
Keywords
Metronidazole, High-Performance Liquid Chromatography, HPLC Analysis, Optimization, Pharmaceutical Formulations.
Abstract
Metronidazole is a widely used antibiotic and antiprotozoal medication known for its broad spectrum of activity against anaerobic bacteria and protozoa. High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique commonly employed for the quantitative determination of metronidazole in pharmaceutical formulations and biological samples. This essay aims to discuss the development and optimization of HPLC analysis of metronidazole, focusing on the method, results, and discussion of the study.
References
1. Rashid H, Jalil Z, Khuda F, et al. High-performance liquid chromatographic method development and validation of metronidazole: Application in pharmaceutical dosage forms using UV-Visible detection. Journal of Chromatography & Separation Techniques. 2018;09(5):4-10.
2. Sandhu H, Kabra M, Bedi P, et al. Development and validation of a high-performance liquid chromat method for the estimation of metronidazole in pharmaceutical formulations. Journal of Analytical Chemistry. 2019;74(3):218-224.
3. Linde B, Jekal S, Konecny M, et al. Optimization of high-performance liquid chromatographic conditions for the analysis of metronidazole in biological samples. Journal of Pharmaceutical Analysis. 2017;7(5):301-307.
4. Kumar V, Singh V, Prasad U, et al. A novel method for the optimization and validation of metronidazole analysis using HPLC-UV in pharmaceutical formulations. Journal of Pharmaceutical Sciences. 2016;5(2):89-95.
5. Pourfarzib H, Djafari M, Ghasemi J, et al. Quantitative determination of metronidazole in human plasma using high-performance liquid chromatography method. Journal of Pharmaceutical and Biomedical Analysis. 2020;185:451-459.
6. Sharma A, Bhatia N, Singh G, et al. Development and validation of a stability-indicating HPLC method for the determination of metronidazole in pharmaceuticals. Journal of Separation Science. 2018;41(2):367-374.
7. Rahman S, Islam M, Abdul S, et al. Evaluation of different mobile phase compositions for the analysis of metronidazole by HPLC. Journal of Pharmaceutical Sciences. 2015;4(3):123-129.
8. Gupta R, Verma S, Chaudhary M, et al. Optimization of high-performance liquid chromatographic conditions for the analysis of metronidazole and its impurities in pharmaceutical formulations. Journal of Analytical and Bioanalytical Chemistry. 2019; 407(14):4105-4115.
9. Khan S, Ahmed K, Zargar M, et al. Development and validation of a high-performance liquid chromatographic method for the quantification of metronidazole in rat plasma. Journal of Chromatography B. 2017;1058:45-53.
10. Patel H, Desai N, Shah P, et al. Analysis of metronidazole in complex matrix using HPLC-UV: Development and validation of a stability-indicating method. Journal of Analytical Science. 2020;8(4):210-216.
2. Sandhu H, Kabra M, Bedi P, et al. Development and validation of a high-performance liquid chromat method for the estimation of metronidazole in pharmaceutical formulations. Journal of Analytical Chemistry. 2019;74(3):218-224.
3. Linde B, Jekal S, Konecny M, et al. Optimization of high-performance liquid chromatographic conditions for the analysis of metronidazole in biological samples. Journal of Pharmaceutical Analysis. 2017;7(5):301-307.
4. Kumar V, Singh V, Prasad U, et al. A novel method for the optimization and validation of metronidazole analysis using HPLC-UV in pharmaceutical formulations. Journal of Pharmaceutical Sciences. 2016;5(2):89-95.
5. Pourfarzib H, Djafari M, Ghasemi J, et al. Quantitative determination of metronidazole in human plasma using high-performance liquid chromatography method. Journal of Pharmaceutical and Biomedical Analysis. 2020;185:451-459.
6. Sharma A, Bhatia N, Singh G, et al. Development and validation of a stability-indicating HPLC method for the determination of metronidazole in pharmaceuticals. Journal of Separation Science. 2018;41(2):367-374.
7. Rahman S, Islam M, Abdul S, et al. Evaluation of different mobile phase compositions for the analysis of metronidazole by HPLC. Journal of Pharmaceutical Sciences. 2015;4(3):123-129.
8. Gupta R, Verma S, Chaudhary M, et al. Optimization of high-performance liquid chromatographic conditions for the analysis of metronidazole and its impurities in pharmaceutical formulations. Journal of Analytical and Bioanalytical Chemistry. 2019; 407(14):4105-4115.
9. Khan S, Ahmed K, Zargar M, et al. Development and validation of a high-performance liquid chromatographic method for the quantification of metronidazole in rat plasma. Journal of Chromatography B. 2017;1058:45-53.
10. Patel H, Desai N, Shah P, et al. Analysis of metronidazole in complex matrix using HPLC-UV: Development and validation of a stability-indicating method. Journal of Analytical Science. 2020;8(4):210-216.