QUANTIFICATION OF COLLAGEN FIBRE MATURITY IN DECELLULARIZED COLLAGEN MATRICES
Main Article Content
Keywords
Transplantation, Collagen, Decellularization.
Abstract
Bioengineering of tissue for transplantation is a growing area. As technology improves it is becoming increasingly possible to produce new cells, tissues and organs to replace damaged or diseased ones. Growing tissues alleviates the reliance on compatible donors and producing immunologically compatible material is possible. Growing new tissues involves the seeding of a supporting structure, or scaffold, with cells taken from the recipient. The scaffold needs to be made of an inert material that doesn’t initiate an immune response, it must have the structure and mechanical properties of the tissue that it is replacing and lastly it must provide an environment that is conducive to cell proliferation. Collagen has emerged as the scaffold material of choice for several reasons: few people possess humoral immunity against it, it is one of the most abundant proteins on earth and, as it is found in many tissues, it can provide a three dimensional structure of the correct spatial arrangement. Preparation of the collagen scaffold involves removal of the cellular content of the tissue leaving behind the extracellular matrix. This is achieved by decellularization using chemical, physical or biological reagents. The decellularization process must remove any material that could illicit a immune response, yet not damage the collagen content or structure. Certain dyes can be used to selectively stain collagen and one of these, picrosirius red, was used here to identify the collagen and study its structure. Samples of three potential scaffolds: tendon, aorta and carotid artery, were examined before and after decellularization to see how the process had affected the amount and structure of the collagen present. It was found that the decellularization process had little effect on the collagen of the tendon or carotid artery, but the aortic collagen was altered by the treatment.
References
1. Almaani, N., Liu, L., Dopping-Hepenstal, P.J.C., Lai-Cheong, J.E., Wong, A., Nanda, A., Moss, C., Martinez, A.E., Mellerio, J.E. and Mcgrath, J.A. (2011) "Identical glycine substitution mutations in type VII collagen may underlie both dominant and recessive forms of dystrophic epidermolysis bullosa", Acta Dermato-Venereologica, vol. 91, no. 3, pp. 262-266.
2. Atala, A., Guzman, L. and Retik, A.B. (1999) "A novel Inert Collagen Matrix For Hypospadias air ", The Journal of urology, vol. 162, no. 3, Part 2, pp. 1148-1150.
3. Badylak, S.F. (2004) "Xenogeneic extracellular matrix as a scaffold for tissue reconstruction", Transplant immunology, vol. 12, no. 3-4, pp. 367-377.
4. Bailey, A.J. (2002) "Changes in bone collagen with age and disease", Journal of Musculoskeletal Neuronal Interactions, vol. 2, no. 6, pp. 529-531.
5. Ballyk, P.D., Walsh, C., Butany, J. and Ojha, M. (1998) "Compliance mismatch may promote graft–artery intimal hyperplasia by altering suture-line stresses", Journal of Biomechanics, vol. 31, no. 3, pp. 229-237.
6. BBC (2010) BBC news: Health, Windpipe transplant success in UK child
7. Available at: http://news.bbc.co.uk/1/hi/8576493.stm [Accessed 20 November 2011].
8. Behmoaras, J., Osborne-Pellegrin, M., Gauguier, D. and Jacob, M. (2005) "Characteristics of the aortic elastic network and related phenotypes in seven inbred rat strains", American Journal of Physiology - Heart and Circulatory Physiology, vol. 288, no. 2 57-2, pp. H769-H777.
9. Bella, J., Eaton, M., Brodsky, B, and Berman, H.M. (1994) "Crystal and molecular structure of a collagen-like peptide at 1.9 A resolution", Science (New York, N.Y.), vol. 266, no. 5182, pp. 75-81.
10. Bella, J., Eaton, M., Brodsky, B., Berman, H.M., (1994)” Crystal and molecular structure of a collagen-like peptide at 1.9 A resolution”, Science, vol. 266, no. 5182, pp. 75-81.
11. Benetos, A., Levy, B.I., Lacolley, P., Taillard, F., Duriez, M. and Safar, M.E. (1997) "Role of angiotensin II and bradykinin on aortic collagen following converting enzyme inhibition in spontaneously hypertensive rats", Arteriosclerosis, thrombosis, and vascular biology, vol. 17, no. 11, pp. 3196-201.
12. Boudko, S.P., Engel, J. and Bachinger, H.P. (2012) "The crucial role of trimerization domains in collagen folding", The international journal of biochemistry & cell biology, vol. 44, no. 1, pp. 21-32.
13. Budd, J.S., Brennan, J., Beard, J.D., Warren, H., Burton, P.R. and Bell, P.R.F. (1990) "Infrainguinal bypass surgery: Factors determining late graft patency", British Journal of Surgery, vol. 77, no. 12, pp. 1382-1387.
14. Buehler, M.J. (2006) "Nature designs tough collagen: Explaining the nanostructure of collagen fibrils", Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 33, pp. 12285-12290.
15. Campbell R.C., (1989) Statistics For Biologists. 3rd ed. Cambridge: Cambridge University Press.
16. Canty, E.G. and Kadler, K.E. (2002) "Collagen fibril biosynthesis in tendon: a review and recent insights", Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, vol. 133, no. 4, pp. 979-985.
17. Chang, K., Hung, L., Chu, I., Ko, C. and Lee, Y. (2010) "The application of type II collagen and chondroitin sulfate grafted PCL porous scaffold in cartilage tissue engineering", Journal of Biomedical Materials Research - Part A, vol. 92, no. 2, pp. 712-723.
18. Chen, G., Ushida, T. and Tateishi, T. (2002) "Scaffold Design for Tissue Engineering", Macromolecular Bioscience, vol. 2, no. 2, pp. 67-77.
19. Chen, R., Ho, H., Tsai, Y. and Sheu, M. (2004) "Process development of an acellular dermal matrix (ADM) for biomedical applications", Biomaterials, vol. 25, no. 13, pp. 2679-2686.
20. Choi, J.S., Kim, B.S., Kim, J.Y., Kim, J.D., Choi, Y.C., Yang, H.-., Park, K., Lee, H.Y. and Cho, Y.W. (2011) "Decellularized extracellular matrix derived from human adipose tissue as a potential scaffold for allograft tissue engineering", Journal of Biomedical Materials Research - Part A, vol. 97 A, no. 3, pp. 292-299.
21. Crapo, P.M., Gilbert, T.W. and Badylak, S.F. (2011) "An overview of tissue and whole organ decellularization processes", Biomaterials, vol. 32, no. 12, pp. 3233-3243.
22. Crapo, P.M. and Wang, Y. (2010) "Physiologic compliance in engineered small-diameter arterial constructs based on an elastomeric substrate", Biomaterials, vol. 31, no. 7, pp. 1626-1635.
23. Dahl, S.L., Koh, J., Prabhakar, V. and Niklason, L.E. (2003) "Decellularized native and engineered arterial scaffolds for transplantation.", Cell transplantation, vol. 12, no. 6, pp. 659-66.
24. Dahms, Piechota, Dahiya, Lue and Tanagho (1998) "Composition and biomechanical properties of the bladder acellular matrix graft: comparative analysis in rat, pig and human", British journal of urology, vol. 82, no. 3, pp. 411-419.
25. Di Lullo, G.A., Sweeney, S.M., Korkko, J., Ala-Kokko, L. and San Antonio, J.D. (2002) "Mapping the ligand-binding sites and disease-associated mutations on the most abundant protein in the human, type I collagen", The Journal of biological chemistry, vol. 277, no. 6, pp. 4223-31.
26. Ding, C., Zhou, Y., He, Y. and Tan, W. (2008) "Perfusion seeding of collagen-chitosan sponges for dermal tissue engineering", Process Biochemistry, vol. 43, no. 3, pp. 287-296.
27. Drez DJ, J., DeLee, J., Holden, J.P., Arnoczky, S., Noyes, F.R. and Roberts, T.S. (1991) "Anterior cruciate ligament reconstruction using bone-patellar tendon-bone allografts. A biological and biomechanical evaluation in goats", The American journal of sports medicine, vol. 19, no. 3, pp. 256-63.
28. Everett IV, T.H. and Olgin, J.E. (2007) "Atrial fibrosis and the mechanisms of atrial fibrillation", Heart Rhythm, vol. 4, no. 3, Supplement, pp. S24-S27.
29. Fitzpatrick, J.C., Clark, P.M. and Capaldi, F.M. (2010) "Effect of decellularization protocol on the mechanical behavior of porcine descending aorta", International journal of biomaterials, vol. 2010, pp. 620503. Epub 2010 Jul 4.
30. Gilbert, T.W., Sellaro, T.L. and Badylak, S.F. (2006) "Decellularization of tissues and organs", Biomaterials, vol. 27, no. 19, pp. 3675-3683.
31. Gillies, A.R., Smith, L.R., Lieber, R.L. and Varghese, S. (2011) "Method for decellularizing skeletal muscle without detergents or proteolytic enzymes", Tissue Engineering - Part C: Methods, vol. 17, no. 4, pp. 383-389.
32. Gonzalez, R.C., Woods, R.E (1993). Digital Image Processing. MA, USA: Addision-Wesley Pub.
33. Grandi, C., Baiguera, S., Martorina, F., Lora, S., Amistà, P., Dalzoppo, D., Del Gaudio, C., Bianco, A., Di Liddo, R., Conconi, M.T. and Parnigotto, P.P. (2011) "Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts", International journal of molecular medicine, vol. 28, no. 3, pp. 315-325.
34. Gui, L., Chan, S.A., Breuer, C.K. and Niklason, L.E. (2010) "Novel utilization of serum in tissue decellularization", Tissue Engineering - Part C: Methods, vol. 16, no. 2, pp. 173-184.
35. Gui, L., Muto, A., Chan, S.A., Breuer, C.K. and Niklason, L.E. (2009) "Development of decellularized human umbilical arteries as small-diameter vascular grafts.", Tissue engineering.Part A, vol. 15, no. 9, pp. 2665-2676.
36. Hadi, A.M., Mouchaers, K.T.B., Schalij, I., Grunberg, K., Meijer, G.A., Vonk-Noordegraaf, A., Van Der Laarse, W.J. and Beliën, J.A.M. (2010) "Rapid quantification of myocardial fibrosis: A new macro-based automated analysis", Analytical Cellular Pathology, vol. 33, no. 5-6, pp. 257-269.
37. Hall, J.K., Banks, G.B., Chamberlain, J.S. and Olwin, B.B. (2010) "Tissue engineering: Prevention of muscle aging by myofiber-associated satellite cell transplantation", Science Translational Medicine, vol. 2, no. p. 57- 83.
38. Harley, B.A., Freyman, T.M., Wong, M.Q. and Gibson, L.J. (2007) "A new technique for calculating individual dermal fibroblast contractile forces generated within collagen-GAG scaffolds", Biophysical journal, vol. 93, no. 8, pp. 2911-2922.
39. Harley, B.A., Spilker, M.H., Wu, J.W., Asano, K., Hsu, H.P., Spector, M. and Yannas, I.V. (2004) "Optimal degradation rate for collagen chambers used for regeneration of peripheral nerves over long gaps", Cells, tissues, organs, vol. 176, no. 1-3, pp. 153-165.
40. Hiss, J., Hirshberg, A., Dayan, D.F., Bubis, J.J. and Wolman, M. (1988) "Aging of wound healing in an experimental model in mice", The American journal of forensic medicine and pathology, vol. 9, no. 4, pp. 310-2.
41. Holbrook, K.A., Byers, P.H. and Pinnell, S.R. (1982) "The structure and function of dermal connective tissue in normal individuals and patients with inherited connective tissue disorders", Scanning electron microscopy, vol. (Pt 4), no. Pt 4, pp. 1731-1744.
42. Holmes, D.F., Gilpin, C.J., Baldock, C., Ziese, U., Koster, A.J. and Kadler, K.E. (2001) "Corneal collagen fibril structure in three dimensions: Structural insights into fibril assembly, mechanical properties, and tissue organization", Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 13, pp. 7307-7312.
43. Holmes, D.F. and Kadler, K.E. (2006) "The 10+4 microfibril structure of thin cartilage fibrils", Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 46, pp. 17249-17254.
44. Holzapfel, G.A. (2006) "Determination of material models for arterial walls from uniaxial extension tests and histological structure", Journal of theoretical biology, vol. 238, no. 2, pp. 290-302.
45. ImageJ (2011) “Segmentation” Available at: http://rsbweb.nih.gov/ij/plugins/index. html#segmentation [Accessed 20 November 2011].
46. Junqueira, L.C., Bignolas, G. and Brentani, R.R. (1979) "Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections", The Histochemical journal, vol. 11, no. 4, pp. 447-55.
47. Junqueira, L.C.\., Montes, G.S.\. and Sanchez, E.M.\. (1982) "The influence of tissue section thickness on the study of collagen by the\ Picrosirius-polarization method.\", Histochemistry\, vol. 74\, no. 1\, pp. 153-6\.
48. Kalathur, M., Baiguera, S. and Macchiarini, P. (2010) "Translating tissue-engineered tracheal replacement from bench to bedside", Cellular and Molecular Life Sciences, vol. 67, no. 24, pp. 4185-4196.
49. Kasimir, M., Rieder, E., Seebacher, G., Silberhumer, G., Wolner, E., Weigel, G. and Simon, P. (2003) "Comparison of different decellularization procedures of porcine heart valves", International Journal of Artificial Organs, vol. 26, no. 5, pp. 421-427.
50. Katsuda, S. and Kaji, T. (2003) "Atherosclerosis and extracellular matrix", Journal of Atherosclerosis and Thrombosis, vol. 10, no. 5, pp. 267-274.
51. Kian Kwan Oo, K., Chen Ong, W., Hui Chi Ang, A., Hutmacher, D.W. and Kim Siang Tan, L. (2007) "Tissue engineered prefabricated vascularized flaps", Head & neck, vol. 29, no. 5, pp. 458-464.
52. Knight, R.L., Wilcox, H.E., Korossis, S.A., Fisher, J. and Ingham, E. (2008) "The use of acellular matrices for the tissue engineering of cardiac valves", Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine, vol. 222, no. 1, pp. 129-143.
53. Korbling, M. and Estrov, Z. (2003) "Adult stem cells for tissue repair - a new therapeutic concept?", The New England journal of medicine, vol. 349, no. 6, pp. 570-82.
54. Korbling, M. and Estrov, Z. (2003) "Adult stem cells for tissue repair - a new therapeutic concept?, The New England journal of medicine, vol. 349, no. 6, pp. 570-582.
55. L.E., F. (2010) "The use of decellularized adipose tissue to provide an inductive microenvironment for the adipogenic differentiation of human adipose-derived stem cells", Biomaterials, vol. 31, no. 17, pp. 4715-4724.
56. Lai, L., Kolber-Simonds, D., Park, K.W., Cheong, H.T., Greenstein, J.L., Im, G.S., Samuel, M., Bonk, A., Rieke, A., Day, B.N., Murphy, C.N., Carter, D.B., Hawley, R.J. and Prather, R.S. (2002) "Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning", Science (New York, N.Y.), vol. 295, no. 5557, pp. 1089-92.
57. Lavker, R.M., Zheng, P.S. and Dong, G. (1986) "Morphology of aged skin", Dermatologic clinics, vol. 4, no. 3, pp. 379-389.
58. Lichtenberg, A., Breymann, T., Cebotari, S. and Haverich, A. (2006) "Cell seeded tissue engineered cardiac valves based on allograft and xenograft scaffolds", Progress in pediatric cardiology, vol. 21, no. 2, pp. 211-217.
59. Lin, T.W., Cardenas, L. and Soslowsky, L.J. (2004) "Biomechanics of tendon injury and repair", Journal of Biomechanics, vol. 37, no. 6, pp. 865-877.
60. Liu, S.Q., Moore, M.M. and Yap, C. (2000) "Prevention of mechanical stretch-induced endothelial and smooth muscle cell injury in experimental vein grafts", Journal of Biomechanical Engineering, vol. 122, no. 1, pp. 31-38.
61. Lu, Q., Ganesan, K., Simionescu, D.T. and Vyavahare, N.R. (2004) "Novel porous aortic elastin and collagen scaffolds for tissue engineering", Biomaterials, vol. 25, no. 22, pp. 5227-5237.
62. Macchiarini, P., Jungebluth, P., Go, T., Asnaghi, M.A., Rees, L.E., Cogan, T.A., Dodson, A., Martorell, J., Bellini, S., Parnigotto, P.P., Dickinson, S.C., Hollander, A.P., Mantero, S., Conconi, M.T. and Birchall, M.A. (2008) "Clinical transplantation of a tissue-engineered airway", The Lancet, vol. 372, no. 9655, pp. 2023-2030.
63. Mays, P.K., Bishop, J.E. and Laurent, G.J. (1988) "Age-related changes in the proportion of types I and III collagen", Mechanisms of ageing and development, vol. 45, no. 3, pp. 203-212.
64. McEniery, C.M. (2006) "Novel therapeutic strategies for reducing arterial stiffness", British journal of pharmacology, vol. 148, no. 7, pp. 881-883.
65. Mercuri, J.J., Gill, S.S. and Simionescu, D.T. (2011) "Novel tissue-derived biomimetic scaffold for regenerating the human nucleus pulposus", Journal of Biomedical Materials Research Part A, vol. 96A, no. 2, pp. 422-435.
66. Meyer, S.R., Chiu, B., Churchill, T.A., Zhu, L., Lakey, J.R.T. and Ross, D.B. (2006) "Comparison of aortic valve allograft decellularization techniques in the rat", Journal of Biomedical Materials Research Part A, vol. 79A, no. 2, pp. 254-262.
67. Motooka, D., Kawahara, K., Nakamura, S., Doi, M., Nishi, Y., Nishiuchi, Y., Kee Kang, Y., Nakazawa, T., Uchiyama, S., Yoshida, T., Ohkubo, T. and Kobayashi, Y. (2011) "The triple helical structure and stability of collagen model peptide with 4(S)-hydroxyprolyl-pro-gly units", Peptide Science, , pp. n/a-n/a.
68. Murase, Y., Narita, Y., Kagami, H., Miyamoto, K., Ueda, Y., Ueda, M. and Murohara, T. (2006) "Evaluation of compliance and stiffness of decellularized tissues as scaffolds for tissue-engineered small caliber vascular grafts using intravascular ultrasound", ASAIO Journal (American Society for Artificial Internal Organs: 1992), vol. 52, no. 4, pp. 450-455.
69. Naso F., Gandaglia A., Formato M., Cigliano A., Lepedda A.J., Gerosa G., Spina M., (2010) “Differential distribution of structural components and hydration in aortic and pulmonary heart valve conduits: Impact of detergent-based cell removal”, Acta Biomater, vol. 6, no.12, pp. 4675-4688.
70. NCBI (2000): Collagen: the Fibrous Proteins of Matrix. Molecular Cell Biology 4th edition. Available at: http://www.ncbi.nlm.nih.gov/books/NBK21582/ [Accessed 20 November 2011].
71. Nieponice, A., Gilbert, T.W. and Badylak, S.F. (2006) "Reinforcement of Esophageal Anastomoses With an Extracellular Matrix Scaffold in a Canine Model", The Annals of Thoracic Surgery, vol. 82, no. 6, pp. 2050-2058.
72. O’Brien, F.J., Harley, B.A., Yannas, I.V. and Gibson, L. (2004) "Influence of freezing rate on pore structure in freeze-dried collagen-GAG scaffolds", Biomaterials, vol. 25, no. 6, pp. 1077-1086.
73. Oikarinen, A., Palatsi, R., Kylmäniemi, M., Keski-Oja, J., Risteli, J. and Kallioinen, M. (1994) "Pachydermoperiostosis: Analysis of the connective tissue abnormality in one family", Journal of the American Academy of Dermatology, vol. 31, no. 6, pp. 947-953.
74. Ophof, R., Maltha, J.C., Von Den Hoff, J.W. and Kuijpers-Jagtman, A. (2004) "Histologic evaluation of skin-derived and collagen-based substrates implanted in palatal wounds", Wound Repair and Regeneration, vol. 12, no. 5, pp. 528-538.
75. Ott, H.C., Matthiesen, T.S., Goh, S.-., Black, L.D., Kren, S.M., Netoff, T.I. and Taylor, D.A. (2008) "Perfusion-decellularized matrix: Using nature's platform to engineer a bioartificial heart", Nature medicine, vol. 14, no. 2, pp. 213-221.
76. Parenteau-Bareil R., Gauvin R., Berthod F., (2010).“Collagen-Based Biomaterials for Tissue Engineering Applications”, Materials, vol. 3, no. 3, pp. 1863-1887.
77. Prather, R.S. (2007) "Targeted genetic modification: xenotransplantation and beyond", Cloning and stem cells, vol. 9, no. 1, pp. 17-20.
78. Quint, C., Arief, M., Muto, A., Dardik, A. and Niklason, L.E. "Allogeneic human tissue-engineered blood vessel", Journal of Vascular Surgery, no. 0.
79. Rabkin, E., Hoerstrup, S.P., Aikawa, M., Mayer JE, J. and Schoen, F.J. (2002) "Evolution of cell phenotype and extracellular matrix in tissue-engineered heart valves during in-vitro maturation and in-vivo remodeling." , The Journal of heart valve disease, vol. 11, no. 3, pp. 308-14; discussion 314.
80. Rashid, S.T., Corbineau, S., Hannan, N., Marciniak, S.J., Miranda, E., Alexander, G., Huang-Doran, I., Griffin, J., Ahrlund-Richter, L., Skepper, J., Semple, R., Weber, A., Lomas, D.A. and Vallier, L. (2010) "Modeling inherited metabolic disorders of the liver using human induced\ pluripotent stem cells." , The Journal of clinical investigation, vol. 120, no. 9, pp. 3127-36.
81. Rich L., Whittaker P., (2005) "Collagen and picrosirius red staining: a polarized light assessment of fibrillar hue and spatial distribution", Braz. J. Morphol. Sci., vol. 22, no. 2, pp. 97-104.
82. Rieder, E., Kasimir, M.T., Silberhumer, G., Seebacher, G., Wolner, E., Simon, P. and Weigel, G. (2004) "Decellularization protocols of porcine heart valves differ importantly in\efficiency of cell removal and susceptibility of the matrix to recellularization with human vascular cells.", The Journal of thoracic and cardiovascular surgery, vol. 127, no. 2, pp. 399-405.
83. Roberts, T.S., Drez, D.J., McCarthy, W. and Paine, R. (1991) "Anterior cruciate ligament reconstruction using freeze-dried, ethylene oxide-sterilized, bone-patellar tendon-bone allografts. Two year results in thirty-six patients", The American journal of sports medicine, vol. 19, no. 1, pp. 35-41.
84. Roy, S., Tsamis, A., Prod'hom, G. and Stergiopulos, N. (2008) "On the in-series and in-parallel contribution of elastin assessed by a structure-based biomechanical model of the arterial wall", Journal of biomechanics, vol. 41, no. 4, pp. 737-43.
85. Rugesan K.M.M ., Palaniswami S., (2010) "Efficient color image segmentation using Multi-Elitist Particle Swarm Optimization Algorithm", International Journal of Computer Science and Network Security, vol. 10, no. 3, pp. 241-245
86. Salacinski, H.J., Goldner, S., Giudiceandrea, A., Hamilton, G., Seifalian, A.M., Edwards, A. and Carson, R.J. (2001) "The mechanical behavior of vascular grafts: a review", Journal of biomaterials applications, vol. 15, no. 3, pp. 241-78.
87. Schechner, J.S., Crane, S.K., Wang, F., Szeglin, A.M., Tellides, G., Lorber, M.I., Bothwell, A.L. and Pober, J.S. (2003) "Engraftment of a vascularized human skin equivalent", The FASEB journal: official publication of the Federation of American Societies for Experimental Biology, vol. 17, no. 15, pp. 2250-6.
88. Schenke-Layland, K., Vasilevski, O., Opitz, F., König, K., Riemann, I., Halbhuber, K.J., Wahlers, T. and Stock, U.A. (2003) "Impact of decellularization of xenogeneic tissue on extracellular matrix integrity for tissue engineering of heart valves", Journal of structural biology, vol. 143, no. 3, pp. 201-208.
89. Shoulders, M.D. and Raines, R.T. (2011) "Interstrand dipole-dipole interactions can stabilize the collagen triple helix", The Journal of biological chemistry, vol. 286, no. 26, pp. 22905-12.
90. Solchaga, L.A., Tognana, E., Penick, K., Baskaran, H., Goldberg, V.M., Caplan, A.I. and Welter, J.F.(2006) "A rapid seeding technique for the assembly of large cell/scaffold composite constructs", Tissue engineering, vol. 12, no. 7, pp. 1851-63.
91. Statcalc3 (2011). Statistics Calculators. Available at: http://www.danielsoper.com/statcalc3/ calc.aspx?id=8 [accessed on 5th December 2011]
92. Tan, G., Dinnes, D.L.M. and Cooper-White, J.J. (2011) "Modulation of collagen II fiber formation in 3-D porous scaffold environments", Acta Biomaterialia, vol. 7, no. 7, pp. 2804-2816.
93. Uitto, J., Fazio, M.J. and Olsen, D.R. (1989) "Molecular mechanisms of cutaneous aging: Age-associated connective tissue alterations in the dermis", Journal of the American Academy of Dermatology, vol. 21, no. 3, Part 2, pp. 614-622.
94. Uygun, B.E., Soto-Gutierrez, A., Yagi, H., Izamis, M., Guzzardi, M.A., Shulman, C., Milwid, J., Kobayashi, N., Tilles, A., Berthiaume, F., Hertl, M., Nahmias, Y., Yarmush, M.L. and Uygun, K. (2010) "Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix", Nature medicine, vol. 16, no. 7, pp. 814-820.
95. Van der Rest, M. and Garrone, R. (1991) "Collagen family of proteins", The FASEB journal: official publication of the Federation of American Societies for Experimental Biology, vol. 5, no. 13, pp. 2814-23.
96. Van Heerebeek, L., Hamdani, N., Handoko, M.L., Falcao-Pires, I., Musters, R.J., Kupreishvili, K., Ijsselmuiden, A.J., Schalkwijk, C.G., Bronzwaer, J.G., Diamant, M., Borbely, A., van der Velden, J., Stienen, G.J., Laarman, G.J., Niessen, H.W. and Paulus, W.J. (2008) "Diastolic stiffness of the failing diabetic heart: importance of fibrosis, advanced glycation end products, and myocyte resting tension", Circulation, vol. 117, no. 1, pp. 43-51.
97. Vavken, P., Joshi, S. and Murray, M.M. (2009) "TRITON-X is most effective among three decellularization agents for ACL tissue engineering", Journal of Orthopaedic Research, vol. 27, no. 12, pp. 1612-1618.
98. Vogel, W., Gish, G.D., Alves, F. and Pawson, T. (1997) "The Discoidin Domain Receptor Tyrosine Kinases Are Activated by Collagen", Molecular cell, vol. 1, no. 1, pp. 13-23.
99. Vranes, D., Cooper, M.E. and Dilley, R.J. (1999) "Cellular Mechanisms of Diabetic Vascular Hypertrophy", Microvascular research, vol. 57, no. 1, pp. 8-18.
100. Whitt J.A., Tedeschi J., McMullan D.R, Del Mundo J.D., Broderick T.L., Carroll C.C., (2010) “Acetaminophen Consumption Alters Achilles Tendon Collagen Content In Wistar Rats”, FASEB J., vol. 24, lb516
101. Williams, C., Liao, J., Joyce, E.M., Wang, B., Leach, J.B., Sacks, M.S. and Wong, J.Y. (2009) "Altered structural and mechanical properties in decellularized rabbit carotid arteries", Acta Biomaterialia, vol. 5, no. 4, pp. 993-1005.
102. Woods, T. and Gratzer, P.F. (2005) "Effectiveness of three extraction techniques in the development of a decellularized bone–anterior cruciate ligament–bone graft", Biomaterials, vol. 26, no. 35, pp. 7339-7349.
103. Xu, H., Raynal, N., Stathopoulos, S., Myllyharju, J., Farndale, R.W. and Leitinger, B. (2011) "Collagen binding specificity of the discoidin domain receptors: Binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1", Matrix Biology, vol. 30, no. 1, pp. 16-26.
104. Yang, X. and Smith, S.L. (2007) "ES cells derived from cloned embryos in monkey - A jump toward human therapeutic cloning", Cell research, vol. 17, no. 12, pp. 969-970.
105. Yannas, I.V., Lee, E., Orgill, D.P., Skrabut, E.M. and Murphy, G.F. (1989) "Synthesis and characterization of a model extracellular matrix that induces partial regeneration of adult mammalian skin", Proceedings of the National Academy of Sciences of the United States of America, vol. 86, no. 3, pp. 933-7.
106. Yoo, J.J., Meng, J., Oberpenning, F. and Atala, A. (1998) "Bladder augmentation using allogenic bladder submucosa seeded with cells", Urology, vol. 51, no. 2, pp. 221-225.
107. Zhao, W., Zhao, T., Chen, Y., Ahokas, R.A. and Sun, Y. (2008) "Oxidative stress mediates cardiac fibrosis by enhancing transforming growth factor-beta1 in hypertensive rats." , Molecular and cellular biochemistry, vol. 317, no. 1-2, pp. 43-50.
108. Zhao, Y., Zhang, Z., Wang, J., Yin, P., Wang, Y., Yin, Z., Zhou, J., Xu, G., Liu, Y., Deng, Z., Zhen, M., Cui, W. and Liu, Z. (2011) "Preparation of decellularized and crosslinked artery patch for vascular tissue-engineering application", Journal of materials science.Materials in medicine, vol. 22, no. 6, pp. 1407-17.
2. Atala, A., Guzman, L. and Retik, A.B. (1999) "A novel Inert Collagen Matrix For Hypospadias air ", The Journal of urology, vol. 162, no. 3, Part 2, pp. 1148-1150.
3. Badylak, S.F. (2004) "Xenogeneic extracellular matrix as a scaffold for tissue reconstruction", Transplant immunology, vol. 12, no. 3-4, pp. 367-377.
4. Bailey, A.J. (2002) "Changes in bone collagen with age and disease", Journal of Musculoskeletal Neuronal Interactions, vol. 2, no. 6, pp. 529-531.
5. Ballyk, P.D., Walsh, C., Butany, J. and Ojha, M. (1998) "Compliance mismatch may promote graft–artery intimal hyperplasia by altering suture-line stresses", Journal of Biomechanics, vol. 31, no. 3, pp. 229-237.
6. BBC (2010) BBC news: Health, Windpipe transplant success in UK child
7. Available at: http://news.bbc.co.uk/1/hi/8576493.stm [Accessed 20 November 2011].
8. Behmoaras, J., Osborne-Pellegrin, M., Gauguier, D. and Jacob, M. (2005) "Characteristics of the aortic elastic network and related phenotypes in seven inbred rat strains", American Journal of Physiology - Heart and Circulatory Physiology, vol. 288, no. 2 57-2, pp. H769-H777.
9. Bella, J., Eaton, M., Brodsky, B, and Berman, H.M. (1994) "Crystal and molecular structure of a collagen-like peptide at 1.9 A resolution", Science (New York, N.Y.), vol. 266, no. 5182, pp. 75-81.
10. Bella, J., Eaton, M., Brodsky, B., Berman, H.M., (1994)” Crystal and molecular structure of a collagen-like peptide at 1.9 A resolution”, Science, vol. 266, no. 5182, pp. 75-81.
11. Benetos, A., Levy, B.I., Lacolley, P., Taillard, F., Duriez, M. and Safar, M.E. (1997) "Role of angiotensin II and bradykinin on aortic collagen following converting enzyme inhibition in spontaneously hypertensive rats", Arteriosclerosis, thrombosis, and vascular biology, vol. 17, no. 11, pp. 3196-201.
12. Boudko, S.P., Engel, J. and Bachinger, H.P. (2012) "The crucial role of trimerization domains in collagen folding", The international journal of biochemistry & cell biology, vol. 44, no. 1, pp. 21-32.
13. Budd, J.S., Brennan, J., Beard, J.D., Warren, H., Burton, P.R. and Bell, P.R.F. (1990) "Infrainguinal bypass surgery: Factors determining late graft patency", British Journal of Surgery, vol. 77, no. 12, pp. 1382-1387.
14. Buehler, M.J. (2006) "Nature designs tough collagen: Explaining the nanostructure of collagen fibrils", Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 33, pp. 12285-12290.
15. Campbell R.C., (1989) Statistics For Biologists. 3rd ed. Cambridge: Cambridge University Press.
16. Canty, E.G. and Kadler, K.E. (2002) "Collagen fibril biosynthesis in tendon: a review and recent insights", Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, vol. 133, no. 4, pp. 979-985.
17. Chang, K., Hung, L., Chu, I., Ko, C. and Lee, Y. (2010) "The application of type II collagen and chondroitin sulfate grafted PCL porous scaffold in cartilage tissue engineering", Journal of Biomedical Materials Research - Part A, vol. 92, no. 2, pp. 712-723.
18. Chen, G., Ushida, T. and Tateishi, T. (2002) "Scaffold Design for Tissue Engineering", Macromolecular Bioscience, vol. 2, no. 2, pp. 67-77.
19. Chen, R., Ho, H., Tsai, Y. and Sheu, M. (2004) "Process development of an acellular dermal matrix (ADM) for biomedical applications", Biomaterials, vol. 25, no. 13, pp. 2679-2686.
20. Choi, J.S., Kim, B.S., Kim, J.Y., Kim, J.D., Choi, Y.C., Yang, H.-., Park, K., Lee, H.Y. and Cho, Y.W. (2011) "Decellularized extracellular matrix derived from human adipose tissue as a potential scaffold for allograft tissue engineering", Journal of Biomedical Materials Research - Part A, vol. 97 A, no. 3, pp. 292-299.
21. Crapo, P.M., Gilbert, T.W. and Badylak, S.F. (2011) "An overview of tissue and whole organ decellularization processes", Biomaterials, vol. 32, no. 12, pp. 3233-3243.
22. Crapo, P.M. and Wang, Y. (2010) "Physiologic compliance in engineered small-diameter arterial constructs based on an elastomeric substrate", Biomaterials, vol. 31, no. 7, pp. 1626-1635.
23. Dahl, S.L., Koh, J., Prabhakar, V. and Niklason, L.E. (2003) "Decellularized native and engineered arterial scaffolds for transplantation.", Cell transplantation, vol. 12, no. 6, pp. 659-66.
24. Dahms, Piechota, Dahiya, Lue and Tanagho (1998) "Composition and biomechanical properties of the bladder acellular matrix graft: comparative analysis in rat, pig and human", British journal of urology, vol. 82, no. 3, pp. 411-419.
25. Di Lullo, G.A., Sweeney, S.M., Korkko, J., Ala-Kokko, L. and San Antonio, J.D. (2002) "Mapping the ligand-binding sites and disease-associated mutations on the most abundant protein in the human, type I collagen", The Journal of biological chemistry, vol. 277, no. 6, pp. 4223-31.
26. Ding, C., Zhou, Y., He, Y. and Tan, W. (2008) "Perfusion seeding of collagen-chitosan sponges for dermal tissue engineering", Process Biochemistry, vol. 43, no. 3, pp. 287-296.
27. Drez DJ, J., DeLee, J., Holden, J.P., Arnoczky, S., Noyes, F.R. and Roberts, T.S. (1991) "Anterior cruciate ligament reconstruction using bone-patellar tendon-bone allografts. A biological and biomechanical evaluation in goats", The American journal of sports medicine, vol. 19, no. 3, pp. 256-63.
28. Everett IV, T.H. and Olgin, J.E. (2007) "Atrial fibrosis and the mechanisms of atrial fibrillation", Heart Rhythm, vol. 4, no. 3, Supplement, pp. S24-S27.
29. Fitzpatrick, J.C., Clark, P.M. and Capaldi, F.M. (2010) "Effect of decellularization protocol on the mechanical behavior of porcine descending aorta", International journal of biomaterials, vol. 2010, pp. 620503. Epub 2010 Jul 4.
30. Gilbert, T.W., Sellaro, T.L. and Badylak, S.F. (2006) "Decellularization of tissues and organs", Biomaterials, vol. 27, no. 19, pp. 3675-3683.
31. Gillies, A.R., Smith, L.R., Lieber, R.L. and Varghese, S. (2011) "Method for decellularizing skeletal muscle without detergents or proteolytic enzymes", Tissue Engineering - Part C: Methods, vol. 17, no. 4, pp. 383-389.
32. Gonzalez, R.C., Woods, R.E (1993). Digital Image Processing. MA, USA: Addision-Wesley Pub.
33. Grandi, C., Baiguera, S., Martorina, F., Lora, S., Amistà, P., Dalzoppo, D., Del Gaudio, C., Bianco, A., Di Liddo, R., Conconi, M.T. and Parnigotto, P.P. (2011) "Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts", International journal of molecular medicine, vol. 28, no. 3, pp. 315-325.
34. Gui, L., Chan, S.A., Breuer, C.K. and Niklason, L.E. (2010) "Novel utilization of serum in tissue decellularization", Tissue Engineering - Part C: Methods, vol. 16, no. 2, pp. 173-184.
35. Gui, L., Muto, A., Chan, S.A., Breuer, C.K. and Niklason, L.E. (2009) "Development of decellularized human umbilical arteries as small-diameter vascular grafts.", Tissue engineering.Part A, vol. 15, no. 9, pp. 2665-2676.
36. Hadi, A.M., Mouchaers, K.T.B., Schalij, I., Grunberg, K., Meijer, G.A., Vonk-Noordegraaf, A., Van Der Laarse, W.J. and Beliën, J.A.M. (2010) "Rapid quantification of myocardial fibrosis: A new macro-based automated analysis", Analytical Cellular Pathology, vol. 33, no. 5-6, pp. 257-269.
37. Hall, J.K., Banks, G.B., Chamberlain, J.S. and Olwin, B.B. (2010) "Tissue engineering: Prevention of muscle aging by myofiber-associated satellite cell transplantation", Science Translational Medicine, vol. 2, no. p. 57- 83.
38. Harley, B.A., Freyman, T.M., Wong, M.Q. and Gibson, L.J. (2007) "A new technique for calculating individual dermal fibroblast contractile forces generated within collagen-GAG scaffolds", Biophysical journal, vol. 93, no. 8, pp. 2911-2922.
39. Harley, B.A., Spilker, M.H., Wu, J.W., Asano, K., Hsu, H.P., Spector, M. and Yannas, I.V. (2004) "Optimal degradation rate for collagen chambers used for regeneration of peripheral nerves over long gaps", Cells, tissues, organs, vol. 176, no. 1-3, pp. 153-165.
40. Hiss, J., Hirshberg, A., Dayan, D.F., Bubis, J.J. and Wolman, M. (1988) "Aging of wound healing in an experimental model in mice", The American journal of forensic medicine and pathology, vol. 9, no. 4, pp. 310-2.
41. Holbrook, K.A., Byers, P.H. and Pinnell, S.R. (1982) "The structure and function of dermal connective tissue in normal individuals and patients with inherited connective tissue disorders", Scanning electron microscopy, vol. (Pt 4), no. Pt 4, pp. 1731-1744.
42. Holmes, D.F., Gilpin, C.J., Baldock, C., Ziese, U., Koster, A.J. and Kadler, K.E. (2001) "Corneal collagen fibril structure in three dimensions: Structural insights into fibril assembly, mechanical properties, and tissue organization", Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 13, pp. 7307-7312.
43. Holmes, D.F. and Kadler, K.E. (2006) "The 10+4 microfibril structure of thin cartilage fibrils", Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 46, pp. 17249-17254.
44. Holzapfel, G.A. (2006) "Determination of material models for arterial walls from uniaxial extension tests and histological structure", Journal of theoretical biology, vol. 238, no. 2, pp. 290-302.
45. ImageJ (2011) “Segmentation” Available at: http://rsbweb.nih.gov/ij/plugins/index. html#segmentation [Accessed 20 November 2011].
46. Junqueira, L.C., Bignolas, G. and Brentani, R.R. (1979) "Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections", The Histochemical journal, vol. 11, no. 4, pp. 447-55.
47. Junqueira, L.C.\., Montes, G.S.\. and Sanchez, E.M.\. (1982) "The influence of tissue section thickness on the study of collagen by the\ Picrosirius-polarization method.\", Histochemistry\, vol. 74\, no. 1\, pp. 153-6\.
48. Kalathur, M., Baiguera, S. and Macchiarini, P. (2010) "Translating tissue-engineered tracheal replacement from bench to bedside", Cellular and Molecular Life Sciences, vol. 67, no. 24, pp. 4185-4196.
49. Kasimir, M., Rieder, E., Seebacher, G., Silberhumer, G., Wolner, E., Weigel, G. and Simon, P. (2003) "Comparison of different decellularization procedures of porcine heart valves", International Journal of Artificial Organs, vol. 26, no. 5, pp. 421-427.
50. Katsuda, S. and Kaji, T. (2003) "Atherosclerosis and extracellular matrix", Journal of Atherosclerosis and Thrombosis, vol. 10, no. 5, pp. 267-274.
51. Kian Kwan Oo, K., Chen Ong, W., Hui Chi Ang, A., Hutmacher, D.W. and Kim Siang Tan, L. (2007) "Tissue engineered prefabricated vascularized flaps", Head & neck, vol. 29, no. 5, pp. 458-464.
52. Knight, R.L., Wilcox, H.E., Korossis, S.A., Fisher, J. and Ingham, E. (2008) "The use of acellular matrices for the tissue engineering of cardiac valves", Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine, vol. 222, no. 1, pp. 129-143.
53. Korbling, M. and Estrov, Z. (2003) "Adult stem cells for tissue repair - a new therapeutic concept?", The New England journal of medicine, vol. 349, no. 6, pp. 570-82.
54. Korbling, M. and Estrov, Z. (2003) "Adult stem cells for tissue repair - a new therapeutic concept?, The New England journal of medicine, vol. 349, no. 6, pp. 570-582.
55. L.E., F. (2010) "The use of decellularized adipose tissue to provide an inductive microenvironment for the adipogenic differentiation of human adipose-derived stem cells", Biomaterials, vol. 31, no. 17, pp. 4715-4724.
56. Lai, L., Kolber-Simonds, D., Park, K.W., Cheong, H.T., Greenstein, J.L., Im, G.S., Samuel, M., Bonk, A., Rieke, A., Day, B.N., Murphy, C.N., Carter, D.B., Hawley, R.J. and Prather, R.S. (2002) "Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning", Science (New York, N.Y.), vol. 295, no. 5557, pp. 1089-92.
57. Lavker, R.M., Zheng, P.S. and Dong, G. (1986) "Morphology of aged skin", Dermatologic clinics, vol. 4, no. 3, pp. 379-389.
58. Lichtenberg, A., Breymann, T., Cebotari, S. and Haverich, A. (2006) "Cell seeded tissue engineered cardiac valves based on allograft and xenograft scaffolds", Progress in pediatric cardiology, vol. 21, no. 2, pp. 211-217.
59. Lin, T.W., Cardenas, L. and Soslowsky, L.J. (2004) "Biomechanics of tendon injury and repair", Journal of Biomechanics, vol. 37, no. 6, pp. 865-877.
60. Liu, S.Q., Moore, M.M. and Yap, C. (2000) "Prevention of mechanical stretch-induced endothelial and smooth muscle cell injury in experimental vein grafts", Journal of Biomechanical Engineering, vol. 122, no. 1, pp. 31-38.
61. Lu, Q., Ganesan, K., Simionescu, D.T. and Vyavahare, N.R. (2004) "Novel porous aortic elastin and collagen scaffolds for tissue engineering", Biomaterials, vol. 25, no. 22, pp. 5227-5237.
62. Macchiarini, P., Jungebluth, P., Go, T., Asnaghi, M.A., Rees, L.E., Cogan, T.A., Dodson, A., Martorell, J., Bellini, S., Parnigotto, P.P., Dickinson, S.C., Hollander, A.P., Mantero, S., Conconi, M.T. and Birchall, M.A. (2008) "Clinical transplantation of a tissue-engineered airway", The Lancet, vol. 372, no. 9655, pp. 2023-2030.
63. Mays, P.K., Bishop, J.E. and Laurent, G.J. (1988) "Age-related changes in the proportion of types I and III collagen", Mechanisms of ageing and development, vol. 45, no. 3, pp. 203-212.
64. McEniery, C.M. (2006) "Novel therapeutic strategies for reducing arterial stiffness", British journal of pharmacology, vol. 148, no. 7, pp. 881-883.
65. Mercuri, J.J., Gill, S.S. and Simionescu, D.T. (2011) "Novel tissue-derived biomimetic scaffold for regenerating the human nucleus pulposus", Journal of Biomedical Materials Research Part A, vol. 96A, no. 2, pp. 422-435.
66. Meyer, S.R., Chiu, B., Churchill, T.A., Zhu, L., Lakey, J.R.T. and Ross, D.B. (2006) "Comparison of aortic valve allograft decellularization techniques in the rat", Journal of Biomedical Materials Research Part A, vol. 79A, no. 2, pp. 254-262.
67. Motooka, D., Kawahara, K., Nakamura, S., Doi, M., Nishi, Y., Nishiuchi, Y., Kee Kang, Y., Nakazawa, T., Uchiyama, S., Yoshida, T., Ohkubo, T. and Kobayashi, Y. (2011) "The triple helical structure and stability of collagen model peptide with 4(S)-hydroxyprolyl-pro-gly units", Peptide Science, , pp. n/a-n/a.
68. Murase, Y., Narita, Y., Kagami, H., Miyamoto, K., Ueda, Y., Ueda, M. and Murohara, T. (2006) "Evaluation of compliance and stiffness of decellularized tissues as scaffolds for tissue-engineered small caliber vascular grafts using intravascular ultrasound", ASAIO Journal (American Society for Artificial Internal Organs: 1992), vol. 52, no. 4, pp. 450-455.
69. Naso F., Gandaglia A., Formato M., Cigliano A., Lepedda A.J., Gerosa G., Spina M., (2010) “Differential distribution of structural components and hydration in aortic and pulmonary heart valve conduits: Impact of detergent-based cell removal”, Acta Biomater, vol. 6, no.12, pp. 4675-4688.
70. NCBI (2000): Collagen: the Fibrous Proteins of Matrix. Molecular Cell Biology 4th edition. Available at: http://www.ncbi.nlm.nih.gov/books/NBK21582/ [Accessed 20 November 2011].
71. Nieponice, A., Gilbert, T.W. and Badylak, S.F. (2006) "Reinforcement of Esophageal Anastomoses With an Extracellular Matrix Scaffold in a Canine Model", The Annals of Thoracic Surgery, vol. 82, no. 6, pp. 2050-2058.
72. O’Brien, F.J., Harley, B.A., Yannas, I.V. and Gibson, L. (2004) "Influence of freezing rate on pore structure in freeze-dried collagen-GAG scaffolds", Biomaterials, vol. 25, no. 6, pp. 1077-1086.
73. Oikarinen, A., Palatsi, R., Kylmäniemi, M., Keski-Oja, J., Risteli, J. and Kallioinen, M. (1994) "Pachydermoperiostosis: Analysis of the connective tissue abnormality in one family", Journal of the American Academy of Dermatology, vol. 31, no. 6, pp. 947-953.
74. Ophof, R., Maltha, J.C., Von Den Hoff, J.W. and Kuijpers-Jagtman, A. (2004) "Histologic evaluation of skin-derived and collagen-based substrates implanted in palatal wounds", Wound Repair and Regeneration, vol. 12, no. 5, pp. 528-538.
75. Ott, H.C., Matthiesen, T.S., Goh, S.-., Black, L.D., Kren, S.M., Netoff, T.I. and Taylor, D.A. (2008) "Perfusion-decellularized matrix: Using nature's platform to engineer a bioartificial heart", Nature medicine, vol. 14, no. 2, pp. 213-221.
76. Parenteau-Bareil R., Gauvin R., Berthod F., (2010).“Collagen-Based Biomaterials for Tissue Engineering Applications”, Materials, vol. 3, no. 3, pp. 1863-1887.
77. Prather, R.S. (2007) "Targeted genetic modification: xenotransplantation and beyond", Cloning and stem cells, vol. 9, no. 1, pp. 17-20.
78. Quint, C., Arief, M., Muto, A., Dardik, A. and Niklason, L.E. "Allogeneic human tissue-engineered blood vessel", Journal of Vascular Surgery, no. 0.
79. Rabkin, E., Hoerstrup, S.P., Aikawa, M., Mayer JE, J. and Schoen, F.J. (2002) "Evolution of cell phenotype and extracellular matrix in tissue-engineered heart valves during in-vitro maturation and in-vivo remodeling." , The Journal of heart valve disease, vol. 11, no. 3, pp. 308-14; discussion 314.
80. Rashid, S.T., Corbineau, S., Hannan, N., Marciniak, S.J., Miranda, E., Alexander, G., Huang-Doran, I., Griffin, J., Ahrlund-Richter, L., Skepper, J., Semple, R., Weber, A., Lomas, D.A. and Vallier, L. (2010) "Modeling inherited metabolic disorders of the liver using human induced\ pluripotent stem cells." , The Journal of clinical investigation, vol. 120, no. 9, pp. 3127-36.
81. Rich L., Whittaker P., (2005) "Collagen and picrosirius red staining: a polarized light assessment of fibrillar hue and spatial distribution", Braz. J. Morphol. Sci., vol. 22, no. 2, pp. 97-104.
82. Rieder, E., Kasimir, M.T., Silberhumer, G., Seebacher, G., Wolner, E., Simon, P. and Weigel, G. (2004) "Decellularization protocols of porcine heart valves differ importantly in\efficiency of cell removal and susceptibility of the matrix to recellularization with human vascular cells.", The Journal of thoracic and cardiovascular surgery, vol. 127, no. 2, pp. 399-405.
83. Roberts, T.S., Drez, D.J., McCarthy, W. and Paine, R. (1991) "Anterior cruciate ligament reconstruction using freeze-dried, ethylene oxide-sterilized, bone-patellar tendon-bone allografts. Two year results in thirty-six patients", The American journal of sports medicine, vol. 19, no. 1, pp. 35-41.
84. Roy, S., Tsamis, A., Prod'hom, G. and Stergiopulos, N. (2008) "On the in-series and in-parallel contribution of elastin assessed by a structure-based biomechanical model of the arterial wall", Journal of biomechanics, vol. 41, no. 4, pp. 737-43.
85. Rugesan K.M.M ., Palaniswami S., (2010) "Efficient color image segmentation using Multi-Elitist Particle Swarm Optimization Algorithm", International Journal of Computer Science and Network Security, vol. 10, no. 3, pp. 241-245
86. Salacinski, H.J., Goldner, S., Giudiceandrea, A., Hamilton, G., Seifalian, A.M., Edwards, A. and Carson, R.J. (2001) "The mechanical behavior of vascular grafts: a review", Journal of biomaterials applications, vol. 15, no. 3, pp. 241-78.
87. Schechner, J.S., Crane, S.K., Wang, F., Szeglin, A.M., Tellides, G., Lorber, M.I., Bothwell, A.L. and Pober, J.S. (2003) "Engraftment of a vascularized human skin equivalent", The FASEB journal: official publication of the Federation of American Societies for Experimental Biology, vol. 17, no. 15, pp. 2250-6.
88. Schenke-Layland, K., Vasilevski, O., Opitz, F., König, K., Riemann, I., Halbhuber, K.J., Wahlers, T. and Stock, U.A. (2003) "Impact of decellularization of xenogeneic tissue on extracellular matrix integrity for tissue engineering of heart valves", Journal of structural biology, vol. 143, no. 3, pp. 201-208.
89. Shoulders, M.D. and Raines, R.T. (2011) "Interstrand dipole-dipole interactions can stabilize the collagen triple helix", The Journal of biological chemistry, vol. 286, no. 26, pp. 22905-12.
90. Solchaga, L.A., Tognana, E., Penick, K., Baskaran, H., Goldberg, V.M., Caplan, A.I. and Welter, J.F.(2006) "A rapid seeding technique for the assembly of large cell/scaffold composite constructs", Tissue engineering, vol. 12, no. 7, pp. 1851-63.
91. Statcalc3 (2011). Statistics Calculators. Available at: http://www.danielsoper.com/statcalc3/ calc.aspx?id=8 [accessed on 5th December 2011]
92. Tan, G., Dinnes, D.L.M. and Cooper-White, J.J. (2011) "Modulation of collagen II fiber formation in 3-D porous scaffold environments", Acta Biomaterialia, vol. 7, no. 7, pp. 2804-2816.
93. Uitto, J., Fazio, M.J. and Olsen, D.R. (1989) "Molecular mechanisms of cutaneous aging: Age-associated connective tissue alterations in the dermis", Journal of the American Academy of Dermatology, vol. 21, no. 3, Part 2, pp. 614-622.
94. Uygun, B.E., Soto-Gutierrez, A., Yagi, H., Izamis, M., Guzzardi, M.A., Shulman, C., Milwid, J., Kobayashi, N., Tilles, A., Berthiaume, F., Hertl, M., Nahmias, Y., Yarmush, M.L. and Uygun, K. (2010) "Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix", Nature medicine, vol. 16, no. 7, pp. 814-820.
95. Van der Rest, M. and Garrone, R. (1991) "Collagen family of proteins", The FASEB journal: official publication of the Federation of American Societies for Experimental Biology, vol. 5, no. 13, pp. 2814-23.
96. Van Heerebeek, L., Hamdani, N., Handoko, M.L., Falcao-Pires, I., Musters, R.J., Kupreishvili, K., Ijsselmuiden, A.J., Schalkwijk, C.G., Bronzwaer, J.G., Diamant, M., Borbely, A., van der Velden, J., Stienen, G.J., Laarman, G.J., Niessen, H.W. and Paulus, W.J. (2008) "Diastolic stiffness of the failing diabetic heart: importance of fibrosis, advanced glycation end products, and myocyte resting tension", Circulation, vol. 117, no. 1, pp. 43-51.
97. Vavken, P., Joshi, S. and Murray, M.M. (2009) "TRITON-X is most effective among three decellularization agents for ACL tissue engineering", Journal of Orthopaedic Research, vol. 27, no. 12, pp. 1612-1618.
98. Vogel, W., Gish, G.D., Alves, F. and Pawson, T. (1997) "The Discoidin Domain Receptor Tyrosine Kinases Are Activated by Collagen", Molecular cell, vol. 1, no. 1, pp. 13-23.
99. Vranes, D., Cooper, M.E. and Dilley, R.J. (1999) "Cellular Mechanisms of Diabetic Vascular Hypertrophy", Microvascular research, vol. 57, no. 1, pp. 8-18.
100. Whitt J.A., Tedeschi J., McMullan D.R, Del Mundo J.D., Broderick T.L., Carroll C.C., (2010) “Acetaminophen Consumption Alters Achilles Tendon Collagen Content In Wistar Rats”, FASEB J., vol. 24, lb516
101. Williams, C., Liao, J., Joyce, E.M., Wang, B., Leach, J.B., Sacks, M.S. and Wong, J.Y. (2009) "Altered structural and mechanical properties in decellularized rabbit carotid arteries", Acta Biomaterialia, vol. 5, no. 4, pp. 993-1005.
102. Woods, T. and Gratzer, P.F. (2005) "Effectiveness of three extraction techniques in the development of a decellularized bone–anterior cruciate ligament–bone graft", Biomaterials, vol. 26, no. 35, pp. 7339-7349.
103. Xu, H., Raynal, N., Stathopoulos, S., Myllyharju, J., Farndale, R.W. and Leitinger, B. (2011) "Collagen binding specificity of the discoidin domain receptors: Binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1", Matrix Biology, vol. 30, no. 1, pp. 16-26.
104. Yang, X. and Smith, S.L. (2007) "ES cells derived from cloned embryos in monkey - A jump toward human therapeutic cloning", Cell research, vol. 17, no. 12, pp. 969-970.
105. Yannas, I.V., Lee, E., Orgill, D.P., Skrabut, E.M. and Murphy, G.F. (1989) "Synthesis and characterization of a model extracellular matrix that induces partial regeneration of adult mammalian skin", Proceedings of the National Academy of Sciences of the United States of America, vol. 86, no. 3, pp. 933-7.
106. Yoo, J.J., Meng, J., Oberpenning, F. and Atala, A. (1998) "Bladder augmentation using allogenic bladder submucosa seeded with cells", Urology, vol. 51, no. 2, pp. 221-225.
107. Zhao, W., Zhao, T., Chen, Y., Ahokas, R.A. and Sun, Y. (2008) "Oxidative stress mediates cardiac fibrosis by enhancing transforming growth factor-beta1 in hypertensive rats." , Molecular and cellular biochemistry, vol. 317, no. 1-2, pp. 43-50.
108. Zhao, Y., Zhang, Z., Wang, J., Yin, P., Wang, Y., Yin, Z., Zhou, J., Xu, G., Liu, Y., Deng, Z., Zhen, M., Cui, W. and Liu, Z. (2011) "Preparation of decellularized and crosslinked artery patch for vascular tissue-engineering application", Journal of materials science.Materials in medicine, vol. 22, no. 6, pp. 1407-17.
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Mar 1, 2023
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How to Cite
Suhailah Khalil Abdulhalim, Rahaf Talal Alqawas, Noha khalid mohammed Nouh, Ohoud Suliman mannaa, Almontaserbellah Abdulhadi Suror, Ala hleel alharbi, Ghazwah Rehim Aljadany, Dr. Abdulaziz Mohammed Felemban, & Bayan Sagga. (2023). QUANTIFICATION OF COLLAGEN FIBRE MATURITY IN DECELLULARIZED COLLAGEN MATRICES. Journal of Population Therapeutics and Clinical Pharmacology, 30(3), 996-1028. https://doi.org/10.53555/jptcp.v30i3.4848
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Author Biographies
Suhailah Khalil Abdulhalim
King Abdullah Medical Complex, Ministry of Health, Jeddah Saudi Arabia
Rahaf Talal Alqawas
Consultant at Molecular diagnostic laboratory at King abdulaziz university hospital
Noha khalid mohammed Nouh
King Abdullah Medical Complex, Ministry of Health, Jeddah Saudi Arabia
Ohoud Suliman mannaa
King Abdullah Medical Complex, Ministry of Health, Jeddah Saudi Arabia
Almontaserbellah Abdulhadi Suror
King Abdullah Medical Complex, Ministry of Health, Jeddah Saudi Arabia
Ala hleel alharbi
Maternity & Children's Hospital in the north , Ministry of Health, Jeddah Saudi Arabia
Ghazwah Rehim Aljadany
King Abdullah Medical Complex, Ministry of Health, Jeddah Saudi Arabia
Dr. Abdulaziz Mohammed Felemban
King abdullah medical complex - jeddah
Bayan Sagga
Ministry of health, Jeddah Saudi Arabia
How to Cite
Suhailah Khalil Abdulhalim, Rahaf Talal Alqawas, Noha khalid mohammed Nouh, Ohoud Suliman mannaa, Almontaserbellah Abdulhadi Suror, Ala hleel alharbi, Ghazwah Rehim Aljadany, Dr. Abdulaziz Mohammed Felemban, & Bayan Sagga. (2023). QUANTIFICATION OF COLLAGEN FIBRE MATURITY IN DECELLULARIZED COLLAGEN MATRICES. Journal of Population Therapeutics and Clinical Pharmacology, 30(3), 996-1028. https://doi.org/10.53555/jptcp.v30i3.4848