“TO STUDY THE PROFILE OF MICROBIAL CONTAMINATION OF CONTACT LENS CASES: A PROSPECTIVE STUDY”.
Main Article Content
Keywords
Contact lens, Case age, Contamination, Micro-organism, Coagulase negative staphylococci
Abstract
Background: The advances in contact lens and lens-care solutions, materials and design have contributed to an increase in contact lens wear, for refractive and aesthetic reasons, to about 140 million users worldwide. Contact lens wear has become increasingly popular for vision correction, yet the risk of microbial contamination remains a significant concern.
Aim and Objective: This prospective study aims to investigate the profile of microbial contamination in contact lens cases and identify potential factors contributing to the presence of microorganisms.
Material and Methods: This was a prospective study carried out in the Department of Ophthalmology and Department of Microbiology. One twenty-three contact lens cases and their case age information were collected from asymptomatic contact lens wearers. Cases of the lens were sampled at two locations, the upper rim and the lower base. The samples then underwent for microbiological investigation at Microbiology Department of Major S D Singh Medical College, Farrukhabad.
Results: In the present study out of 123 lens cases, contamination of contact lenses occurred in 56% (69 of 123) of cases. The most frequent micro-organism isolated were Coagulase negative Staphylococcus aureus (55.07%), in gram negative Pseudomonas aeruginosa (13.04%) and in fungi Candida species(7.2%) were isolated. It was also noted that a large number of gram-negative organism recovered from upper rim than the lower rim of flat well lens cases. Lens cases < 3 months of age had lower number of contamination rate than cases aged more than 9 months.
Conclusion: The findings of this research may contribute to the development of effective strategies for minimizing microbial contamination in contact lens cases, ultimately enhancing the safety and well-being of contact lens wearers.
References
2. Stapleton F, Keay L, Edwards K, Naduvilath T, Dart JK, Brian G, Holden BA. The incidence of contact lens-related microbial keratitis in Australia. Ophthalmology 2008;115:1655–62.
3. Schein OD, Poggio EC. Ulcerative keratitis in contact lens wearers. Incidence and risk factors. Cornea 1990; 9(Suppl 1):S55–8.
4. Dart JK, Radford CF, Minassian D, Verma S, Stapleton F. Risk factors for microbial keratitis with contemporary contact lenses: a case-control study. Ophthalmology 2008;115:1647–54, 1654.e1–3.
5. Whitcher JP, Srinivasan M, Upadhyay MP. Microbial keratitis. In : Johnson GJ, Minassian DC, Weale RA, West SK, editors. The epidemiology of eye diseases. 2nd ed. London: Arnold; 2003. p. 190-5
6. Radford CF, Minassian DC, Dart JK. Acanthamoeba keratitis in England and Wales: incidence, outcome, and risk factors. Br J Ophthalmol 2002; 86:536–42.
7. Mah-Sadorra JH, Yavuz SG, Najjar DM, Laibson PR, Rapuano CJ, Cohen EJ. Trends in contact lens-related corneal ulcers. Cornea 2005; 24 : 51-8.
8. Mayo MS, Schlitzer RL, Ward MA, Wilson LA, Ahearn DG. Association of Pseudomonas and Serratia corneal ulcers with use of contaminated solutions. J Clin Microbiol 1987;25:1398–400.
9. Corrigan KM, Harmis NY, Willcox MD. Association of Acinetobacter species with contact lens-induced adverse responses. Cornea. 2001; 20:463– 466.
10. Yung MS, Boost M, Cho P, Yap M. Microbial contamination of contact lenses and lens care accessories of soft contact lens wearers (university students) in Hong Kong. Ophthalmic Physiol Opt 2007; 27 : 11-21.
11. Jalbert I, Willcox MD, Sweeney DF. Isolation of Staphylococcus aureus from a contact lens at the time of a contact lens-induced peripheral ulcer: Case report. Cornea. 2000; 19:116–120.
12. Keay L, Harmis N, Corrigan K, et al. Infiltrative keratitis associated with extended wear of hydrogel lenses and abiotrophia defectiva. Cornea. 2000;19:864– 869.
13. Sankaridurg PR, Sharma S, Willcox M, et al. Colonization of hydrogel lenses with Streptococcus pneumoniae: Risk of development of corneal infiltrates. Cornea. 1999; 18:289–295.
14. Sankaridurg PR, Vuppala N, Sreedharan A, et al. Gram negative bacteria and contact lens induced acute red eye. Indian J Ophthalmol. 1996; 44:29–32.
15. Sankaridurg PR, Willcox MD, Sharma S, et al. Haemophilus influenzae adherent to contact lenses associated with production of acute ocular inflammation. J Clin Microbiol. 1996;3 4:2426–2431.
16. Ibrahim I A-J, AL-Hadaria SA, Fayidh MA. Bacterial contamination of contact lenses among some female students and employees of College of Education Ibn AL Haitham, University of Baghdad. Ibn Al Haitham J pure Appl sci 2008; 21 : 9-22
17. Devonshire P, Munro FA, Abernethy C, Clark BJ. Microbial contamination of contact lens cases in the west of Scotland. Br J Ophthalmol 1993; 77:41–5.
18. Yung MS, Boost M, Cho P, Yap M. Microbial contamination of contact lenses and lens care accessories of soft contact lens wearers (university students) in Hong Kong. Ophthalmic Physiol Opt 2007; 27:11–21.
19. Rosenthal RA, Stein JM, McAnally CL, Schlech BA. A comparative study of the microbiologic effectiveness of chemical disinfectants and peroxide-neutralizer systems. CLAO J 1995; 21:99–110.
20. Stapleton F, Dart JK, Seal DV, Matheson M. Epidemiology of Pseudomonas aeruginosa keratitis in contact lens wearers. Epidemiol Infect 1995; 114:395–402.
21. Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH, Hanna BA. Manual of clinical microbiology, 8th edition:manual of clinical microbiology. Clini Infect Dis. 2004; 38(8):1199–1200.
22. Lakkis C, Fleiszig SM. Resistance of Pseudomonas aeruginosa isolates to hydrogel contact lens disinfection correlates with cytotoxic activity. J Clin Microbiol 2001; 39:1477–86.
23. Das S, Sheoreym H, Taylor HR, et al. Association between cultures of contact lens and corneal scraping in contact lens related microbial keratitis. Arch Ophthalmol. 2007; 125:1182–1185.
24. Martins EN, Farah ME, Alvarenga LS, et al. Infectious keratitis: Correlation between corneal and contact lens cultures. CLAO J. 2002; 28:146–148.
25. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing: twenty second informational supplement M100–S22. Wayne: CLSI; 2008.
26. Donzis PB, Mondino BJ, Weissman BA, Bruckner DA. Microbial contamination of contact lens care systems. Am J Ophthalmol 1987; 104:325–33.
27. Wu YT, Zhu H, Harmis NY, Iskandar SY, Willcox M, Stapleton F. Profile and frequency of microbial contamination of contact lens cases. Optom Vis Sci. 2010; 87(3):E152-E158.
28. Mowrey-McKee MF, Monnat K, Sampson HJ, Smith CM, Davies GA, Mandt L, Proskin HM. Microbial contamination of hydrophilic contact lenses. Part I: Quantitation of microbes on patient worn-andhandled lenses. CLAO J 1992; 18:87–91.
29. Mowrey-McKee MF, Sampson HJ, Proskin HM. Microbial contamination of hydrophilic contact lenses. Part II: Quantitation of microbes after patient handling and after aseptic removal from the eye. CLAO J 1992; 18:240–4.
30. Yung MS, Boost M, Cho P, Yap M. Microbial contamination of contact lenses and lens care accessories of soft contact lens wearers (university students) in Hong Kong. Ophthalmic Physiol Opt. 2007; 27(1):11–21.
31. Velasco J, Bermudez J. Comparative study of the microbial flora on contact lenses, in lens cases, and in maintenance liquids. Int Contact Lens Clin 1996; 23:55–8.
32. Gopinathan U, Stapleton F, Sharma S, Willcox MD, Sweeney DF, Rao GN, Holden BA. Microbial contamination of hydrogel contact lenses. J Appl Microbiol 1997; 82:653–8.
33. Szczotka-Flynn LB, Imamura Y, Chandra J, et al. Increased resistance of contact lens-related bacterial biofilms to antimicrobial activity of soft contact lens care solutions. Cornea. 2009;28(8):918–926.
34. Hart DE, Reindel W, Proskin HM, Mowrey-McKee MF. Microbial contamination of hydrophilic contact lenses: quantitation and identification of microorganisms associated with contact lenses while on the eye. Optom Vis Sci 1993; 70:185–91.
35. U. S. Department of Health and Human Services, U. S. Food and Drug Administration. Focusing on Contact LensSafety.Availableat:http://www.fda.gov/ForConsumers/ConsumerUpdates/ucm048893.htm. Accessed June 27, 2009.
36. Lakkis C, Anastasopoulos F, Terry C, Borazjani R. Time course of the development of contact lens case and contact lens contamination. Invest Ophthalmol Vis Sci 2009; 50:E-Abstract 6352