AMELIORATIVE AND PHYTO-THERAPEUTIC POTENTIAL OF ANCIENT MEDICINAL PLANTS TO MANAGE REPRODUCTIVE HEALTH IN MALE ALBINO RATS
Main Article Content
Keywords
Nicotine, oxidative stress, infertility, reproductive problems, polyherbal preparation, therapeutic agent
Abstract
The use of nicotine is increasing particularly in young generation which leads to the development of different diseases. Reproductive problem in male and female is also one of them because nicotine directly interferes with antioxidant mechanism, reproductive hormonal axis and destroyed the reproductive organ histology. This research was planned to explore the ameliorative and phytotherapeutic potential of selected medicinal plants including M. pruriens, C. behen and P. embilica hydroethanolic extracts preparation as Polyherbal preparation (PHP) in albino male rats. PHP was evaluated for phytochemicals, antioxidant activities, In-vitro spermatozoa parameters and In-vivo study in male albino rats after induction of infertility by nicotine. The results revealed the presence of wide range phytomolecules including tannins, flavonoids, saponins, alkaloids, triterpenes, and glycosides; TPC (56.13 ± 1.39 mgGAE/g), and TFC (18.915±1.417 μgCE/g dry). HPLC results showed different important phenolic compounds while FTIR analysis reveals many peaks for the presence of functional molecules. The results of hemolytic and thrombolytic activities explored significant (p<0.05) protective role of selected medicinal plants. In-vitro spermatozoa parameters study revealed that PHP has significant (p<0.05) potential on the motility and viability of sperms. The results of in-vivo study explored that PHP treatment significantly (p<0.05) restore the antioxidant status after intoxication with nicotine. A significant (p<0.05) improvement in the hepatic like ALT, AST and renal parameters including urea, creatinine and uric acid was also found. Moreover, the results of reproductive hormones including LH, FSH, testosterone and histological study of testis tissues explored significant (p<0.05) therapeutic impact of PHP treatment as compared to control groups. It could be concluded that selected medicinal plants have significant antioxidant as well as reproductive problems management properties that can help potential uses in traditional medicines as a therapeutic agent. However, trial at high level then clinical trials is required to declare this formation as a safe drug.
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2. Acharya, C.K., Madhu, N.R., Khan, N.S. and Guha, P., (2021). Improved reproductive efficacy of Phyllanthus emblica L. (Gaertn.) on testis of male Swiss mice and a pilot study of its potential values. International Journal of Food Sciences and Nutrition, 10(4), pp.7-14.
3. Ahmad, B., Hafeez, N., Rauf, A., Bashir, S., Linfang, H., Rehman, M. U., ... & Rengasamy, K. R. (2021). Phyllanthus emblica: A comprehensive review of its therapeutic benefits. South African journal of botany, 138, 278-310.
4. Ahmed, H., Shah, S.A., Rashid, A., Ayaz, S. and Rahat, S., (2023). Phyto-pharmacological studies on ethanolic extract of pueraria tuberosa and its effect on spermatozoa parameters. Journal of Population Therapeutics and Clinical Pharmacology, 30(19), pp.1044-1061.
5. Albayrak, S., Atasagun, B., & Aksoy, A. (2017). Comparison of phenolic components and biological activities of two Centaurea sp. obtained by three extraction techniques. Asian Pacific journal of tropical medicine, 10(6), 599-606.
6. Arun, S., Burawat, J., Yannasithinon, S., Sukhorum, W., Limpongsa, A. and Iamsaard, S., (2018). Phyllanthus emblica leaf extract ameliorates testicular damage in rats with chronic stress. Journal of Zhejiang University. Science. B, 19(12), p.948.
7. Ashidi, J.S., Owagboriaye, F.O., Yaya, F.B., Payne, D.E., Lawal, O.I. and Owa, S.O., (2019). Assessment of reproductive function in male albino rat fed dietary meal supplemented with Mucuna pruriens seed powder. Heliyon, 5(10).
8. Atta, A., Shahid, M., Kanwal, Z., Jafri, S.A., Riaz, M., Xiao, H., Abbas, M., Egbuna, C. and SimalGandara, J., (2023). Inhibition of oxidative stress and advanced glycation end‐product formation in purified BSA/glucose glycation system by polyphenol extracts of selected nuts from Pakistan. Food Science & Nutrition, 11(6), pp.3414-3421.
9. Boniface, F., Washa, W.B. and Nnungu, S., (2024). Comparison of nutritional values of Mucuna pruriens L. (velvet bean) seeds with the most preferred legume pulses. Food Production, Processing and Nutrition, 6(1), p.17.
10. Burtis, C. A., & Ashwood, E. R. (1994). Tietz textbook of clinical chemistry. Amer Assn for Clinical Chemistry.
11. Çelikezen, F.Ç., Hayta, Ş., Özdemir, Ö. and Türkez, H., (2019). Cytotoxic and antioxidant properties of essential oil of Centaurea behen L. in vitro. Cytotechnology, 71, pp.345-350.
12. Chen, A., Bookstein, J. J., & Meldrum, D. R. (1991). Diagnosis of a testosterone-secreting adrenal adenoma by selective venous catheterization. Fertility and sterility, 55(6), 1202-1203.
13. Chougule, P., Pawar, R., Limaye, D., Joshi, Y. M., & Kadam, V. (2012). In-Vitro antioxidant activity of ethanolic extract of Centaurea behen. Journal of Applied Pharmaceutical Science, 4, 106-110.
14. Concessao, P.L., Bairy, K.L. and Raghavendra, A.P., (2023). Ameliorating effect of Mucuna pruriens seed extract on sodium arsenite-induced testicular toxicity and hepato-renal histopathology in rats. Veterinary World, 16(1), p.82.
15. Deli, M., Nguimbou, R.M., Djantou, E.B., Tatsadjieu Ngoune, L. and Njintang Yanou, N., (2020). Bioactive compounds of velvet bean (Mucuna pruriens L.) seeds. Bioactive Compounds in Underutilized Vegetables and Legumes, pp.1-19.
16. Faghihi, M., Hosseini, J., Esmaeili, S., Movahhed. M. and Sepidarkish, M. (2018). Natural Pregnancy in Infertile Couples Using Red and White Bahman Root Extract Retrieved of Iranian Traditional Medicine Due to Semen Parameters Improvement - A Case Report Study”, International Journal of Pharmaceutical and Phytopharmacological Research, 8(6), pp.105-109.
17. Fatima, G., Siddiqui, A., Jamal, A., & Chaudhary, S. (2019). Health promoting properties of Behman safed, a root of Centaurea behen Linn. -a review. Journal of Drug Delivery and Therapeutics, 9(4-s), 657-660.
18. Fatima, H., Shahid, M., Pruitt, C., Pung, M.A., Mills, P.J., Riaz, M. and Ashraf, R., (2022). Chemical fingerprinting, antioxidant, and anti-inflammatory potential of hydroethanolic extract of Trigonella foenum-graecum. Antioxidants, 11(2), p.364.
19. Frank, J. E., Faix, J. E., Hermos, R. J., Mullaney, D. M., Rojan, D. A., Mitchell, M. L., & Klein, R. Z. (1996). Thyroid function in very low birth weight infants: effects on neonatal hypothyroidism screening. The Journal of pediatrics, 128(4), 548-554.
20. Gopumadhavan, S., Rafiq, M., Azeemuddin, M., & Mitra, S. K. (2008). Ameliorative effect of Party Smart in rat model of alcoholic liver disease. Indian Journal of Experimental Biology. 46(2):132-7.
21. Gottfredsen, R.H., Larsen, U.G., Enghild, J.J. and Petersen, S.V., (2013). Hydrogen peroxide induce modifications of human extracellular superoxide dismutase that results in enzyme inhibition. Redox biology, 1(1), pp.24-31.
22. Iamsaard, S., Kietinun, S., Sattayasai, J., Bunluepuech, K., Wu, A.T.H. and Choowong-In, P., (2023). Prevention of seminal vesicle damage by Mucuna pruriens var. pruriens seed extract in chronic unpredictable mild stress mice. Pharmaceutical Biology, 61(1), pp.89-99.
23. Jain, S., Jain, A., Vaidya, A., Kumar, D., & Jain, V. (2014). Preliminary phytochemical, pharmacognostical and physico-chemical evaluation of Cedrus deodara heartwood. Journal of Pharmacognosy and Phytochemistry, 3(1).
24. Keser, S., Celik, S., Turkoglu, S., Yilmaz, O., & Turkoglu, I. (2012). Hydrogen peroxide radical scavenging and total antioxidant activity of hawthorn. Chemistry Journal, 2(1), 9-12.
25. Khan, M.R. and Ahmed, D., (2009). Protective effects of Digera muricata (L.) Mart. on testis against oxidative stress of carbon tetrachloride in rat. Food and Chemical Toxicology, 47(6), pp.1393-1399.
26. Khan, M.R., Khan, G.N. and Ahmed, D., (2011). Evaluation of antioxidant and fertility effects of Digera muricata in male rats. African Journal Pharmaceutical Pharmacology, 5(6), pp.688-699.
27. Lanka, S. (2018). A review on pharmacological, medicinal and ethnobotanical important plant: Phyllanthus emblica Linn. (syn. Emblica officinalis). World Journal of Pharmaceutical Research, 7(4), 380-396.
28. Lapyuneyong, N., Tangsrisakda, N., Choowong-In, P., Chaisiwamongkol, K., Uabundit, N., Sawatpanich, T., Arun, S., Wu, A.T.H. and Iamsaard, S., (2022). Seed extract of Thai Mucuna pruriens reduced male reproductive damage in rats induced by chronic stress. Pharmaceutical Biology, 60(1), pp.374-383.
29. Marinova, G., & Batchvarov, V. (2011). Evaluation of the methods for determination of the free radical scavenging activity by DPPH. Bulgarian Journal of Agricultural Science, 17(1), 11-24.
30. Mirunalini, S., & Krishnaveni, M. (2010). Therapeutic potential of Phyllanthus emblica (amla): the ayurvedic wonder. Journal of basic and clinical physiology and pharmacology, 21(1), 93-105. 31. Munir, N., Mahmood, Z., Shahid, M., Afzal, M.N., Jahangir, M., Ali Shah, S.M., Tahir, I.M., Riaz, M., Hussain, S., Akram, M. and Yousaf, F., (2022). Withania somnifera chemical constituents’ in vitro antioxidant potential and their response on spermatozoa parameters. Dose-Response, 20(1), p.15593258221074936.
32. Murthy, K. and Mishra, S.H., (2016). Velvet bean roots stimulates humoral and cell mediated immunity and offers protection against cyclophosphamide induced myelosuppression. International Journal Phytomedicine, 8(1), pp.69-79.
33. Onigemoa, M., Dairo, F.A., Oso, Y. and Onigemo, H., (2022). Proximate composition and phytonutrients of heat-treated loofah gourd Luffa cylindrica (MJ Roem) seeds. Int J Sci Eng Res, 13, pp.770-775.
34. Owen, L., McNeill, A. and Callum, C., (1998). Trends in smoking during pregnancy in England, 1992-7: quota sampling surveys. Bmj, 317(7160), pp.728-730.
35. Panteghini, M., & Ceriotti, F. (2000). Establishing a reference system in clinical enzymology. biochimica clinica, 24(6), 499-508.
36. Pranuthi, E. K., Narendra, K., Swathi, J., Sowjanya, K. M., Reddi, K. R., Emmanuel, R. F. S., & Satya, A. K. (2014). Qualitative assessment of bioactive compounds from a very rare medicinal plant ficus dalhousiae miq. Journal of Pharmacognosy and Phytochemistry, 3(1).
37. Qiu, Q., Kuo, A., Todd, H., Dias, J. A., Gould, J. E., Overstreet, J. W., & Lasley, B. L. (1998). Enzyme immunoassay method for total urinary follicle-stimulating hormone (FSH) beta subunit and its application for measurement of total urinary FSH. Fertility and sterility, 69(2), 278-285.
38. Quranayati, Q., Saidi, N., Nurliana, N., Idroes, R., Gusti, N. and Nasution, R., (2023). Effect of Phyllanthus emblica L. stem bark extract on diabetic nephropathy and hyperlipidemia in rats. Journal of Pharmacy & Pharmacognosy Research, 11(2), pp.308-314.
39. Quranayati, Q., Saidi, N., Nurliana, N., Idroes, R., Gusti, N. and Nasution, R., (2023). Effect of Phyllanthus emblica L. stem bark extract on diabetic nephropathy and hyperlipidemia in rats. Journal of Pharmacy & Pharmacognosy Research, 11(2), pp.308-314.
40. Rahman, T.U., Khan, H., Liaqat, W. and Zeb, M.A., (2022). Phytochemical screening, green synthesis of gold nanoparticles, and antibacterial activity using seeds extract of Ricinus communis L. Microscopy Research and Technique, 85(1), pp.202-208.
41. Riaz, M., Mahmood, Z., Shahid, M., Saeed, M.U.Q., Tahir, I.M., Shah, S.A., Munir, N. and ElGhorab, A., (2016). Impact of reactive oxygen species on antioxidant capacity of male reproductive system. International journal of immunopathology and pharmacology, 29(3), pp.421-425.
42. Rudolph, L.M., Bentley, G.E., Calandra, R.S., Paredes, A.H., Tesone, M., Wu, T.J. and Micevych, P.E., (2016). Peripheral and central mechanisms involved in the hormonal control of male and female reproduction. Journal of neuroendocrinology, 28(7).
43. Sahreen, S., Khan, M. R., Khan, R. A., & Shah, N. A. (2013). Effect of Carissa opaca leaves extract on lipid peroxidation, antioxidant activity and reproductive hormones in male rats. Lipids in Health and disease, 12(1), 1-10.
44. Saini, R., Sharma, N., Oladeji, O. S., Sourirajan, A., Dev, K., Zengin, G., ... & Kumar, V. (2022). Traditional uses, bioactive composition, pharmacology, and toxicology of Phyllanthus emblica fruits: A comprehensive review. Journal of ethnopharmacology, 282, 114570.
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Dec 31, 2023
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How to Cite
Mahvish Ajaz, Syed Muhammad Ali Shah, Abid Rashid, Rehana Dilshad, & Naveed Munir. (2023). AMELIORATIVE AND PHYTO-THERAPEUTIC POTENTIAL OF ANCIENT MEDICINAL PLANTS TO MANAGE REPRODUCTIVE HEALTH IN MALE ALBINO RATS. Journal of Population Therapeutics and Clinical Pharmacology, 30(19), 1939-1955. https://doi.org/10.53555/jptcp.v30i19.4093
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Author Biographies
Mahvish Ajaz
Department of Eastern Medicine, Government College University, Faisalabad-Pakistan
Syed Muhammad Ali Shah
Department of Eastern Medicine, Government College University, Faisalabad-Pakistan
Abid Rashid
Directorate of Medical Sciences, Faculty of Life Sciences, Government College University, Faisalabad-Pakistan
Rehana Dilshad
Shaikh Zaid Medical College Rahim Yar Khan.
Naveed Munir
Department of Biomedical Laboratory Sciences, School of Health Sciences, University of Management and Technology, Lahore-Pakistan.
How to Cite
Mahvish Ajaz, Syed Muhammad Ali Shah, Abid Rashid, Rehana Dilshad, & Naveed Munir. (2023). AMELIORATIVE AND PHYTO-THERAPEUTIC POTENTIAL OF ANCIENT MEDICINAL PLANTS TO MANAGE REPRODUCTIVE HEALTH IN MALE ALBINO RATS. Journal of Population Therapeutics and Clinical Pharmacology, 30(19), 1939-1955. https://doi.org/10.53555/jptcp.v30i19.4093