“RENOPROTECTIVE ACTIVITY OF AMORPHOPHALLUS PAEONIIFOLIUS AGAINST GENTAMICIN-INDUCED NEPHROTOXICITY IN RATS”
Main Article Content
Keywords
Amorphophallus paeoniifolius, nephro-protective, Gentamicin, antioxidant
Abstract
To investigate the nephroprotective and antioxidant activities of methanol and aqueous extracts of Amorphophallus paeoniifolius on Gentamicin induced nephrotoxicity in male Wistar rats.In this model of nephrotoxicity, 24 adults male wistar rats (150-200gms) were evenly divided into 4 groups. Group-1 and 2 served as control and Gentamicin induced models respectively, while Group-3 and 4 are the treatment groups which were simultaneously treated with methanolic (250mg/kg) and aqueous (250 mg/kg) extracts of Amorphophallus paeoniifolius respectively, after each dose of Gentamicin (80 mg/kg, i.p.) for 10 days. On 11th day, blood samples for biochemical parameters, while the rat kidneys for histology were obtained under inhaled diethyl ether anesthesia.Co-administration of ME and AE extracts with Gentamicin decreased rise in serum urea and creatinine. Amorphophallus paeoniifolius extracts significantly show increased activities of renal Catalase and Glutathione, and significant decreased activity of lipid peroxidase. Apart from these, histopathological changes also showed the protective nature of Amorphophallus paeoniifolius extracts against Gentamicin induced necrotic damage of renal tissues.In comparison to aqueous extracts, histological and biochemical findings demonstrated that the methanolic extract of Amorphophallus paeoniifolius conferred greater nephro-protective and antioxidant effects against Gentamicin-induced nephrotoxicity in rats. In the future, Amorphophallus paeoniifolius might provide a clue for the development of a brand-new medication to treat drug-induced nephrotoxicity.
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2. Naznin Ara, Hasan Nur. In-vitro antioxidant activity of methanolic leaves and flowers extract of Lippia alba. Res J M Med Sci 2009; 4(1):107-110.
3. Jain PK, Agrawal RK. Antioxidant and free radical scavenging properties of developed mono and polyherbal formulations. Asian J Exp Sci 2008; 22 (3): 213-220.
4. Mark Percival. Antioxidants. Clinical nutrition insights 1998; 1-4.
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6. Anne Waugh, Allison Grant. Anatomy and Physiology in Health and Illness. 10th ed. London: Churchill Livingstone Elsevier; 2006. p. 337-338.
7. Kidney [Internet]. Cited 2012 May 03. Available from: http://en.wikipedia.org/wiki/Kidney.
8. Arthur C Guyton. Text book of Medical Physiology. 10th ed. Singapore: Harcourt Publishers International Company; 2000. p. 264-379.
9. Herfindal, Gourley. Text book of Therapeutic Drug and Disease Management. 7th ed. London: Charcil Livingstone; 2000. p. 425-36.
10. Arthur C Guyton. Text book of Medical Physiology. 11th ed. Singapore: Harcourt Publishers International Company; 2006. p. 314-315.
11. The Formation of Urine [Internet]. Cited 2012 March 20. Available from: http://www.nsbri.org/humanphysspace/focus4/ep-urine.html.
12. Gerard J Tortora, Bryan H Derrickson. Principles of Anatomy and Physiology.12th ed. USA: John Wiley and Sons Inc; 2009. p. 1035-1048.
13. Barry M, Brenner, Floyd C, Rector. The Kidney. 6th ed, Vol 1, Philadelphia: WB Saunders Company; 2000. p. 3-67.
14. Paul L Munson. Principles of Pharmacology, Basic concepts and clinical applications. New York: Chapman and Hall; 1996. p. 685.
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16. Goodman, Gilman, editors. The pharmacological basis of Therapeutics. 10th ed.Newyork: Mcgraw Hill Medical Publishing; 2001. p. 1229-433.
17. Vijay Kumar K, Naidu MUR, Anwar A, Shifow, Ratnakar KS. Probucol protects against gentamian induced nephrotoxicity in rats. Ind J Pharmacol 2000; 32:108- 113.
18. Mahadev Rao, Rao MNA. Protective effect of selcomethionine against Cisplatin induced renal toxicity in mice and rats. J Pharm Pharmacol 1998; 50:687-91.
19. Marieke Kruidering, Bob Van De Water, Emile De Heer, Gerard J Mulder, Fred Nagelkerke J. Cisplatin-induced nephrotoxicity in porcine proximal tubular cells: Mitochondiral dysfunction by inhibition of complexes I to IV of the respiratory chain. J Pharmacol Exp Thera 1997; 280(2):638-49.
20. Devi Priya S, Shyamala Devi CS. Protective effect of quercetin in Cisplatin induced cell injury in the rat kidney. Ind J Pharmacol 1999; 31:422-26.
21. Brain S Cummings, Jane Mc Howat, Rick G Schnellmann. Role of endoplasmic reticulum Ca2+ -independent phospholipase A2 in Cisplatin-induced renal cell apoptosis. J Pharmacol Exp Thera 2004; 308(3):921-28.
22. Pierre Marche, Sophie Koutouzov, Arlette Girard. Impairment of membrane phosphoinositide metabolism by aminoglycoside anti-biotics: Steptomycin, Amikacin, Kanamycin, Dibekacin, Gentamicin and Enomycin. J Pharmacol Exp Thera 1983; 227(2):415-20.
23. Matthew J Bartosiewicz, David Jenkins, Sharron Penn, Jennifer Emery, Alan Buckpitt. Unique gene expression patterns in liver and kidney associated with exposure to chemical toxicants. J Pharmacol Exp Thera 2001; 297(3):895-905.
24. Raoef Imamdi, Marjo De Graauw, Bob Van De Water. Protein kinase C mediates Cisplatin-induced loss of adherens junctions followed by apoptosis of renal proximal tubular epithelial cells. J Pharmacol Exp Thera 2004; 311(3):892-903.
25. Tetsuo Yasumasu, Toyofumi Ueda, Jiro Uozumi, Yukitaka Mihara, Yasuhiro Koikawa, Joichi Kumazawa. Ultrastructural alterations and DNA synthesis of renal cell nuclei following Cisplatin or carboplatin injection in rats. J Pharm Pharmacol 1992; 44:885-87.
26. Masuda H, Tanaka T, Takahama U. Cisplatin generates superoxide anion by interaction with DNA in a cell free system. Biochem Biophys Res Commun 1994; 1175-80.
27. Sdzuka Y, Shoji T, Takinoy. Effects of Cisplatin on the activities of enzymes which protects against lipid peroxidation. Biochem Pharmacol 1992; 43:1872-75.
28. Hostetler KY, Hall LB. Inhibition of kidney lysosomal phospholipases A and C by aminoglycoside antibiotics: Possible mechanism of aminoglycoside toxicity. Proc Natl Acad Sci 1982; 79:1663.
29. Cullis PR, Dekruijff B, Hope MJ, Nayar R, Schmid SL. Phospholipids and membrane transport. Can J Biochem 1980; 58(10):1091-100.
30. Hebert SC, Sun A. Hypotonic cell volume in mouse medullary thick ascending limb: Effect of ADH. Amn J Physiol 1988; 255:F962-F969.
31. Arends MJ, Morris RG, Wyllie AH. Apoptosis. The role of endonuclease. Amn J Pathol 1990; 136(3):593-608.
32. Dobyan DC, Levi J, Jacobs LC, Kosek J, Weiner MW. Mechanism of Cis- platinum nephrotoxicity: II morphological observations. J Pharmacol Expt Ther 1980; 213(3):551-6.
33. Beales D, Hue DP, Mclean AEM. Lipid peroxidation, protein synthesis and protection by calcium EDTA in paracetamol injury to isolated hepatocytes. Biochem Pharmacol 1985; 34(1):19-23.
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Shekshavali T, Abdul Haseeb TS, T. Naga Aparna, Jaffer sadik Md, Konda Shravan kumar, V. Arun Reddy, E. Praveen, & Shaik Rahaman. (2022). “RENOPROTECTIVE ACTIVITY OF AMORPHOPHALLUS PAEONIIFOLIUS AGAINST GENTAMICIN-INDUCED NEPHROTOXICITY IN RATS”. Journal of Population Therapeutics and Clinical Pharmacology, 29(03), 464-492. https://doi.org/10.53555/jptcp.v29i03.3898
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Author Biographies
Shekshavali T
Assistant Professor, Department of Pharmacognosy, National College of pharmacy, shivmogga, Karnataka
Abdul Haseeb TS
Senior Pharmacist, Pharmacy service Department, Johns Hopkins Aramco Healthcare, Dharan, Saudi Arabia
T. Naga Aparna
Associate Professor, Department of Pharmaceutics, Sri indu institute of Pharmacy, Hyderabad, Telangana, India
Jaffer sadik Md
Associate Professor, Department of Pharmaceutics, Sri indu institute of Pharmacy, Hyderabad, Telangana, India
Konda Shravan kumar
Professor, Department of Pharmaceutics, Vision college of Pharmaceutical sciences and research, Hyderabad, Telangana, India
V. Arun Reddy
Assistant Professor, Department of Pharmaceutical analysis, Bharat institute of Technology (Pharmacy), Mangalpaly, Hyderbad,India
E. Praveen
Assistant Professor, Department of Pharmaceutics, Sri indu institute of Pharmacy, Hyderabad, Telangana, India
Shaik Rahaman
Department of Pharmacology, School of Pharmaceutical Education and research, Jamia Hamdard University, New Delhi, India
How to Cite
Shekshavali T, Abdul Haseeb TS, T. Naga Aparna, Jaffer sadik Md, Konda Shravan kumar, V. Arun Reddy, E. Praveen, & Shaik Rahaman. (2022). “RENOPROTECTIVE ACTIVITY OF AMORPHOPHALLUS PAEONIIFOLIUS AGAINST GENTAMICIN-INDUCED NEPHROTOXICITY IN RATS”. Journal of Population Therapeutics and Clinical Pharmacology, 29(03), 464-492. https://doi.org/10.53555/jptcp.v29i03.3898