ADVANCEMENTS OF ENZYME PURIFICATION
Main Article Content
Keywords
enzyme purification, aqueous two phases, hybrid nanoflowers, three phase partioning
Abstract
The goal of enzyme purification is to obtain a substantial amount of a specific enzyme while conserving its function in vivo. Enzyme purification consists of three phases, which can be elaborated into four general steps. Depending on the physical and chemical properties, downstream application, and the source of the target enzyme, each enzyme requires its unique purification strategy. The purpose of purification is to isolate specific enzymes from a crude extract of cells containing many other unwanted components in order to obtain the maximum specific activity with the best possible recovery of the initial activity. The following article discusses enzyme purification techniques and the advancements of enzyme purification are also discussed.
References
2. Chapman J, Ismail A, Dinu C. Industrial Applications of Enzymes: Recent Advances, Techniques, and Outlooks. Catalysts. 2018; 8(6):238. doi:10.3390/catal8060238
3. Boman, H.G., Björk, W. (1963). General Aspects of Enzyme Purification and Characterization. In: Modern Methods of Plant Analysis/Moderne Methoden der Pflanzenanalyse, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-94878-7_22
4. Meghwanshi GK, Kaur N, Verma S, et al. Enzymes for pharmaceutical and therapeutic applications. Biotechnol Appl Biochem. 2020;67(4):586-601. doi:10.1002/bab.1919
5. Saxena L, BK Iyer, L Ananthanarayan (2007) Three phase partitioning as a novel method for purification of ragi (Eleusine coracana) bifunctional amylase/protease inhibitor. Process Biochemistry 42: 491-495.
6. Gagaoua M (2014) Three-phase partitioning as an efficient method for the purification and recovery of ficin from Mediterranean fig (Ficus carica L.) latex. Separation and Purification Technology 132: 461-467.
7. Gagaoua M, Hoggas N, Hafid K (2015) Three phase partitioning of zingibain, a milk-clotting enzyme from Zingiber officinale Roscoe rhizomes. Int J Biol Macromol 73: 245-252.
8. Rawdkuen S (2010) Three-phase partitioning of protease from Calotropis procera latex. Biochemical Engineering Journal 50: 145-149.
9. Rajendran, D.S., Chidambaram, A., Kumar, P.S. et al. Three-phase partitioning for the separation of proteins, enzymes, biopolymers, oils and pigments: a review. Environ Chem Lett 21, 911–934 (2023).
10. Yi Yu, Xu Fei, Jing Tian, Longquan Xu, Xiuying Wang, Yi Wang, Self-assembled enzyme–inorganic hybrid nanoflowers and their application to enzyme purification, Colloids and Surfaces B: Biointerfaces, Volume 130, 2015, Pages 299-304,ISSN 0927-7765.
11. Shamraja S. Nadar, Rohini G. Pawar, Virendra K. Rathod, Recent advances in enzyme extraction strategies: A comprehensive review, International Journal of Biological Macromolecules,Volume 101, 2017, Pages 931-957, ISSN 0141-8130.
12. Van Horn, W. D., Ogilvie, M. E., & Flynn, P. F. (2009). Reverse Micelle Encapsulation as a Model for Intracellular Crowding. In Journal of the American Chemical Society (Vol. 131, Issue 23, pp. 8030–8039). American Chemical Society (ACS).
13. Melo EP, Aires-Barros MR, Cabral JM. Reverse micelles and protein biotechnology. Biotechnol Annu Rev. 2001;7:87-129. doi: 10.1016/s1387-2656(01)07034-x. PMID: 11686050
Article Sidebar
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
Urvashi Midha
PhD scholar, Department of Biochemistry, Santosh Medical College and Hospital, Santosh Deemed to be University, Ghaziabad, Uttar Pradesh, India
Dr. Divya Shukla
Tutor, Department of Biochemistry, LLRM Medical College, Meerut, Uttar Pradesh, India
Dr. Luna Sinha
Assistant Professor, Department of Biochemistry, Santosh Medical College and Hospital, Santosh Deemed to be University, Ghaziabad, Uttar Pradesh, India
Dr. Juhi Aggarwal
Professor and Head of the Department, Department of Biochemistry, Santosh Medical College and Hospital, Santosh Deemed to be University, Ghaziabad, Uttar Pradesh, India