EVALUATION OF OXIDATIVE STRESS AND VITAMIN A IN PATIENTS OF CHRONIC OTITIS MEDIA WITH AND WITHOUT CHOLESTEATOMA.
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Abstract
Background: Chronic otitis media is a serious clinical condition characterized by an accumulation of fluid behind the tympanic membrane, while lacking the signs and symptoms of acute infection. The aim of this study was to investigate the effect of oxidative stress and antioxidant situation on chronic otitis media (COM) with and without cholesteatoma.
Methodology: The study included a total of 75 cases of COM and further subcategorised based on cholesteatoma, 35 cases COM with cholesteatoma (20 females and 15 males) and 35 COM without cholesteatoma (16 females and 19 males). Serum specimens were taken prior to surgery and diseased tissue specimens from the ear were obtained during surgery from all patients. A proper audiometry testing was done. serum specimens were taken from every individual. The malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GHPx) were measured in the serum samples of the patient of COM with and without cholesteatoma. Vitamin A were determined by HPLC methodology.
Results: The patients in this study, who might be of either gender, ranged in age from 25 to 40. Patients with chronic otitis media were subdivided based on their cholesteatoma status, as well as the levels of different oxidative indicators and vitamin A. Table 1 displays information about each groups' age, gender, and BMI. Table 2 displays antioxidant enzymes as well as additional biochemical information for both groups.
Conclusion: The present study could help clarify the fundamental causes of the pathophysiology of COM. A finding of vitamin A does not show any direct role in occurrence of diseases but it shown significant difference in COM with and without cholesteatoma.
References
2. Louw L. Acquired cholesteatoma pathogenesis: stepwise explanations. J Laryngol Otol. 2010 Jun;124(6):587-93.
3. Sagiroglu S, Ates S, Tolun FI, Oztarakci H. Evaluation of oxidative stress and antioxidants effect on turning process acute otitis media to chronic otitis media with effusion. Niger J Clin Pract. 2019;22(3):375-379. doi:10.4103/njcp.njcp_126_18.
4. Yilmaz T, Kocan EG, Besler HT, Yilmaz G, Gursel B. The role of oxidants and antioxidants in otitis media with effusion in children. Otolaryngol Head Neck Surg. 2004 Dec;131(6):797-803.
5. Shukla GK, Mahajan A, Pandey S, Gujrati VR, Vrat S, Mishra SC, et al. A study of free radicals and scavenging enzyme in tonsillitis. Boll Chim Farm. 1996 Dec;135(11):653-5
6. Sagiroglu, S; Ates, S1; Tolun, FI2; Oztarakci, H3. Evaluation of Oxidative Stress and Antioxidants Effect on Turning Process Acute Otitis Media to Chronic Otitis Media with Effusion. Nigerian Journal of Clinical Practice 22(3):p 375-379, March 2019. | DOI: 10.4103/njcp.njcp_126_18
7. Baysal E, Aksoy N, Kara F, Taysi S, Taskin A, Bilinc H, et al. Oxidative stress in chronic otitis media. Eur Arch Otorhinolaryngol. 2013 Mar; 270(4):1203-8. 12. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39(1):44-84.
8. Shukla MK, Mahajan A, Pandey S, Gujrati VR, Vrat S, Mishra SC, et al. A study of free radicals and scavenging enzyme in tonsillitis. Boll Chim Farm. 1995 Dec;135(11):656.
9. Yoshioka T, Kawada K, Shimada T, Mori M. Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol. 1979 Oct;135(3): 372-6
10. Martanegara IF, Purwanto B, Boesoirie SF. Characteristic of intratemporal complication in chronic suppurative otitis media patient with and without cholesteatoma at hasan sadikin general hospital bandung. J Med Heal. 2020;2(6):197–204.
11. Arya IP, Pratama A, Sudipta IM, Andi K, Saputra D. Description of patients with chronic suppurative otitis media at sanglah hospital, Denpasar, 2014–2016. E Jurnal Med. 2019;8(4):1
12. Gaurano JL, Joharjy IA. Middle ear cholesteatoma: characteristic CT findings in 64 patients. Ann Saudi Med. 2004;24(6):442–447. doi:10.5144/ 0256-4947.2004.442 2
13. Yariktas M, Doner F, Dogru H, Yasan H, Delibas N. The role of free oxygen radicals on the development of otitis media with effusion. Int J Pediatr Otorhinolaryngol. 2004 Jul;68(7):889-94.
14. Garcia Callejo FJ, Estors Ferrero J, Morant Ventura A, Segarra Cortes P, Velert Vila MM. Lipoperoxidation in otorrhea of the middle ear as a marker of infection: clinical application. Acta Otorrinolaringol Esp. 2000 Aug-Sep;51(6):478-81
15. Baysal E, Aksoy N, Kara F, Taysi S, Taskin A, Bilinc H, et al. Oxidative stress in chronic otitis media. Eur Arch Otorhinolaryngol. 2013 Mar; 270(4):1203-8
16. Lee GY, Han SN. The role of vitamin E in immunity. Nutrients. 2018;10(11):1–18. doi:10.3390/nu10111614
17. Schmitz J, West KP, Khatry SK, et al. Vitamin A supplementation in preschool children and risk of hearing loss as adolescents and young adults in rural Nepal: randomised trial cohort follow-up study. BMJ. 2012;344(7841):1–12. doi:10.1136/bmj.d7962
18. Greaves RF, Woollard GA, Hoad KE, et al. Laboratory medicine best practice guideline: vitamins A, E and the carotenoids in blood. Clin Biochem Rev. 2014;35(2):81–113. doi:10.1186/bcr2338
19. Arulselvan P, Fard MT, Tan WS, et al. Role of antioxidants and natural products in inflammation. Oxid Med Cell Longev. 2016;2016. doi:10.1155/ 2016/5276130
20. Mehmet Fatih Garça, Mahfuz Turan, Barış Avşa, Ferhat Kalkan2 ·Halit Demir, Ahmet Kozan, Nazım Bozan. The Evaluation of Oxidative Stress in the Serum and Tissue Specimens of Patients With Chronic Otitis Media. Clinical and Experimental Otorhinolaryngology. 2015; 8 (2): 97-101.
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Dr. Nilamadhaba Prusty
Assistant Professor, Department of ENT, FM MCH, Balasore
Dr. Biswaranjan Prusty
Assistant Professor, Department of Medicine, DD MCH, Keonjhar
Dr. Siddharth Panditray
Consultant, ENT & Head and Neck Surgery, AHPGIC, Cuttack