IMMUNO-STIMULATING ACTIONS OF GARLIC (ALLIUM SATIVUM) ON HAEMATOLO-IMMUNOLOGICAL PARAMETERS, INTESTINAL MICRO-FLORA AND GROWTH PERFORMANCE IN LABEO ROHITA (ROHU)
Main Article Content
Keywords
Vaccination, Chemotherapeutic Agents, Immunity Haematology, Lysozyme, Microbial Load
Abstract
Vaccination and use of other chemotherapeutic agents in fishes is not valued to perform because of its residual effects and method of delivery. It has turned out as essential to use such cheap chemically active compounds that are produced naturally, have not any side effects or residual effect on fish immunity and are easy to deliver. With the rising and commercialization of aqua-culture production, contagious diseases and occasional increase in death are major causes of heavy loss in aqua-culture enterprises. The effects of any disease in fish depend upon some specific types of factors. These Factors are categorized as; diseases and sources of these disease-causing agents, environmental stress, the level or degree of environmental stress upon the fish being effect, physical appearance and physiological condition and genetic resistance of host fish against all the manipulations cause by disease. The aim of conducted study was to determine changes in immunological parameters gut micro-flora of Labeo rohita (Rohu) against different supplementary doses of dietary garlic. Fishes were divided into different groups (control group, feed on garlic free feed and treatment groups-1, 2 and 3 feed on diet with inclusion of 5g, 10g and 15g garlic). Duration of the research trials was 90 days. Results of the present examination signified that the utilization of dietary garlic supplementation at different levels in Labeo rohita (Rohu) feed significantly (P ≤ O.O5) enhanced all parameters regarding growth, development and improved the ability to utilize feed more effectively. FCR, SGR, FW and FI parameters were also observed significantly increased in all groups that received garlic supplemented feed as compare to control group. Results of hematological changes demonstrated significant increase (P ≤ 0.05) in RBCs, WBCs, Hb, PCV levels. Serums bactericidal activities and lysozyme activities were observed remarkably higher in all fish fed on garlic-additive feedings as compared to the control group. Highest values of bactericidal activities and least viable colony count were prominently evident in each treatment group which received garlic supplementations in feed as compared to the control group. The comprehensive study regarding the effects of garlic on different parameters of growth, haematology, immunity and gut microbial load suggested the use of garlic supplements and residues in fish diet to support higher and consistence aqua-culture production with minimum losses due to unexpected break out of diseases and environmental stresses
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Sumaira Abbas
Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore Pakistan
Asia Iqbal
Department of Wildlife and Ecology, Faculty of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore Pakistan
Shahbaz Anwari
Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore Pakistan.
Kashif Ali
Department of Zoology, Division of Science and Technology, University of Education Lahore 54770, Pakistan
Ayesha Riaz
Department of Zoology, GC Women University Faisalabad, Pakistan
Amina Ayub
Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Subcampus Depalpur, Okara
Muhammad Farhan Nasir
Department of Zoology, Division of Science and Technology, University of Education Lahore 54770, Pakistan.
Rida Amjad
Department of Zoology, Government Sadiq College Women University Bahawalpur
Maham Saleem
Department of Zoology, Division of Science and Technology, University of Education Lahore 54770, Pakistan