EVALUATION OF THE EFFECT OF AQUEOUS AND ETHANOL EXTRACT OF HYDROCOTYLE SIBTHORPIOIDES LEAF ON WOUND HEALING
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Keywords
Wound, excision, Hydrocotyle sibthorioides, extract, toxicity
Abstract
The objective of the present investigation was to evaluate the wound healing action of Hydrocotyle sibthorpioides leaf extract in rats. The extraction abilities of different solvents for recovering extractable components from leaves followed the order: ethanol >water>pet ether. The acute toxicity study suggests a LD50 of more than 2000 mg/Kg for both the ethanolic and aqueous extracts of the plant. The ethanolic and aqueous extracts of Hydrocotyle sibthorpioides leaves were evaluated for the in vivo wound healing effect by the excision model. The topical application of 5 % w/w of the Hydrocotyle sibthorpioides resulted in an enhanced and statistically significant (p < 0.05) wound healing activity in vivo when using the ethanolic extract. The wound healing by the aqueous extract was not significant in comparison to the control group. The ointment containing plant extract (ethanolic) exhibited 98.56 ± 3.61 % contraction of wound on the 21st day whereas only 63.99 ± 6.33 % contraction of wound was found in the control animals. The results revealed by aqueous extract were also significant as it could reduce the wound area by 88.89 ± 6.33 % on the 21st day.
References
2. Hutchinson J. The Wound Programme. Centre for Medical Education: Dundee, UK. 1992
3. Pasparakis M, Haase I, Nestle FO. Mechanisms regulating skin immunity and inflammation. Nature Reviews Immunology, vol. 14, no. 5, pp. 289–301, 2014.
4. Przekora A. A Concise Review on Tissue Engineered Artificial Skin Grafts for Chronic Wound Treatment: Can We Reconstruct Functional Skin Tissue In Vitro?. Cells 2020, 9. DOI: 10.3390/cells9071622.
5. Kasarla R, Elumalai A, Eswaraiah MC, Ravi P, Naresh V. An annual review on wound-healing medicinal plants. J Nat Prod Plant Resour 2012, 2(1): 182.
6. Lakshmi MS, Rani KS, Reddy TU. A review on diabetes mellitus and the herbal plants used for its treatment, Asian Journal of Pharmaceutical and Clinical Research 2012, 5, 15-21.
7. Hazarika I, Mukundan GK, Sivakami Sundari P, Laloo D. Journey of Hydrocotyle sibthorpioides Lam.: From traditional utilization to modern therapeutics—A review. Phytotherapy Research. 2020; 1-25. DOI: 10.1002/ptr.6924
8. Sahira Banu K, Cathrine L. General Techniques Involved in Phytochemical Analysis. International Journal of Advanced Research in Chemical Sciences 2015, 2, 4, 25-32
9. Arora P, Arora V. Preliminary phytochemical screening of crude drugs In: A Textbook of Herbal Drug Technology, Pee Vee Books, Pubjab 2019, pp 179-180
10. https://ntp.niehs.nih.gov/iccvam/suppdocs/feddocs/oecd/oecd_gl423.pdf; assessed on 17/03/2023
11. Singh B, Jain A. Evaluation of wound healing action of Delonix regia leaf extract in rats. Journal of Pharmacology and Biomedicine. 2021; 5(4): 374-382
12. Morton JJP, Malone MH. Evaluation of vulnerary activity by an open wound procedure in rats. Arch. Int. Pharmacodyn 1972, 196: 117-126.
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Sudhakar Tyagi
IPS College of Pharmacy, Gwalior, Madhya Pradesh, India
Sandeep Jain
IPS College of Pharmacy, Gwalior, Madhya Pradesh, India
Bharat Kumar Tyagi
IPS College of Pharmacy, Gwalior, Madhya Pradesh, India