PHYTOSYNTHESIS, CHARACTERIZATION AND PHARMACOLOGICAL INVESTIGATION OF GOLD NANOPARTICLES USING LEAVES OF ACACIA MODESTA WALL
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Keywords
Phytosynthesis, A. modesta Wall, Characterization, AuNPs, Antimicrobial, Antileshmanial, Cytotoxic
Abstract
Aqueous leaves extracts of Acacia modesta Wall. was utilized to phyto-synthesize green AuNPs. The leaves of A. modesta were investigated to possess potential phytochemicals such as alkaloids, flavonoids, polyphenols, reducing sugars, tannins, saponins and steroids, which help in the bioconversion of Au+3 to Auo. The synthesized AuNPs were analyzed to be stable nano-crystalline spherical, having a size span of 20 – 100 nm. The λmax peak for AuNPs was observed at 550 nm, which manifests the precise fabrication of AuNPs. The sharp Auo peak, along with other organic elements, i.e., C, N, O, Ca++, Mg++, Na+ and Cl- at variable intensities in EDX analysis, manifest phytochemicals in plant extracts have effectively reduced the ionic Au by acting as efficient biocapping and bioreducing agent. The pharmacological activities of these AuNPs in comparison to aqueous leaves extract manifest that AuNPs possess excellent antifungal activity against F. solani (92%), F. oxysporum (91%), M. fur fur (90%), Penecillium and C. albicans (89%). Moderate to good antibacterial activity was analyzed against S. aureus (62%) and K. pnuemoniae (53%). Less cytotoxic activity was observed against brine shrimp nauplii i.e. 6.67% at higest sample concentration i.e. 1000 µg/mL. Good anti-leshmanial activity was manifested by green AuNPs against L. major, showing IC50 value of 50.2 + 0.01 respectively.
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Taj Muhammad
Sarhad institute of Allied health Science, Sarhad University of Science and Information Technology, Peshawar Pakistan
Aamir Aziz
Sarhad institute of Allied health Science, Sarhad University of Science and Information Technology, Peshawar Pakistan
Awais Khalid
Department of Physics, Hazara University Mansehra, Khyber Pakhtunkhwa 21300 Pakistan
Bader S. Alotaibi
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Al- Quwayiyah, Shaqra University, Riyadh, Saudi Arabia
Nain Taara Bukhari
Department of Microbiology, Women University Swabi, Pakistan
Iqbal Nisa
Department of Microbiology, Women University Swabi, Pakistan
Fawad Khan
Department of Microbiology,Hazara University Manshera, Pakistan
Nabila Qayum
Centre for Biotechnology and Microbiology, University of Swat, Pakistan
Yosra Modafer
Department of Biology, College of Science, Jazan University, Jazan 45142, Saudi Arabia
Farah Shireen
Sarhad institute of Allied health Science, Sarhad University of Science and Information Technology, Peshawar Pakistan
Muhammad Saqib Khalil
Sarhad institute of Allied health Science, Sarhad University of Science and Information Technology, Peshawar Pakistan