STUDY OF CORRELATION OF SEROLOGICAL MARKERS OF DENGUE VIRUS INFECTION WITH PLATELET COUNT AT A TERTIARY CARE HOSPITAL OF CENTRAL INDIA
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Keywords
Seropositivity, Serological markers, Venipuncture, platelet count
Abstract
Aim: -To confirm serologically, dengue infection in febrile patients who are clinically symptomatic by using ELISA to detect the presence of NS1 antigen and IgM antibodies.
-To correlate the platelet count with the serological markers of dengue virus infection.
Materials and Methods: This prospective study conducted for a period of one year in Department of Microbiology, Gajra Raja Medical College and J. A. Group of Hospitals (JAH), Gwalior, Madhya Pradesh and the study period extended from January 2018 to 31st December 2018 This study was performed among patients with febrile illness who were clinically symptomatic for dengue infection and referred to the Department of Microbiology, Gajra Raja Medical College, Gwalior for investigation of dengue virus infection during the study period of one year.The minimum blood samples required for the study were 350 samples. Blood samples from seronegative patients were taken as control for comparison of platelet count in fever patients.
A structured assessment form was used to obtain the clinical history regarding febrile illness including clinical symptoms and signs. Blood samples from clinically symptomatic IPD patients were collected by venipuncture under sterile precautions in different department of J.A. Group of Hospital and sent to the Department of Microbiology as soon as possible.
Results- A total number of 1,762 samples of clinically suspected dengue fever was processed for serological markers of dengue in department of microbiology during the period of one year from January 2018 to December 2018. Out of the 1,762, 568 cases were positive for serological tests, Prevalence of dengue seropositivity in clinically suspected dengue patients was 32.22%.
Conclusion- Dengue is presently regarded globally as the most important and rapidly spreading mosquito-borne viral disease. It is a cause of great concern to public health in India. Every year, thousands of peoples are affected and contribute to the burden of health care. In the present study patients with febrile illness and clinically symptomatic for dengue were investigated during January 2018 to December 2018.
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Dr. Abhi Mishra
Post Graduate Medical Officer, Department of Microbiology, P.C. Sethi Govt. Hospital, A.B. Road ,Indore (M.P.)
Dr. Ashish Saraswat
Senior Resident , Department of Microbiology , M.L.B. Medical College Jhansi,(U.P.)
Dr. Priyanka Gupta
Senior Resident, Department of Microbiology, UCMS & GTB Hospital, New Delhi (India)
Dr. Deepak Gawali
Demonstrator, Department of Microbiology, MGM Medical College, Indore,(M.P.)
Dr. Ashok Kumar Rastogi
Associate Professor, Department of Forensic Medicine & Toxicology, All India Institute of Medical Sciences, Patna (Bihar)
Dr. Seema Sharma
Senior Resident, Department of Obstetrics & Gynecology, M.L.B. Medical College Jhansi,(U.P.)