AMELIORATIVE EFFECT OF QUERCETIN ON LIPID, ENZYMATIC, HORMONAL AND BIOCHEMICAL PROFILING OF STZ-INDUCED DIABETIC RATS.

Abstract

Herbal medicine was commonly used before the development of other medicine with an increase in their scope of use each year. Camellia sinensis (L.) leaves important phyto-constituents like quercetin. In current study, therapeutic effects of intraperitoneally induced quercetin (10mg/mL/Kg b.w.) was checked to cure streptozotocin-induced (55mg/mL/Kg b.w.) diabetes in albino Wistar rats of both genders (300- 400 g), by keeping metformin (250mg/mL/Kg b.w.) as positive control, followed by the estimation of total lipids, cholesterol, triglycerides, HDL, LDL, VLDL, AST, ALT, ALP, , amylase, lipase, LDH ,urea, creatinine, uric acid, bilirubin, albumin, globulin, A/G ratio, total proteins, sodium, potassium, calcium, magnesium, chloride and HbA1clevels by kit methods. Statistically analyzed results (at p≤ 0.05) revealed that quercetin have improved the levels of cholesterol (88.25±10.47 and 47.3±3.0 mg/dL), triglycerides (46.00±5.72 and 50±8.0 mg/dL), HDL (57.50±1.29 and 26.3±2.0 mg/dL), LDL (29.75±6.95 and 16.3±1.0 mg/dL), VLDL (16.50±1.29 and 32.3±1.2 mg/dL), Urea (3.1±0.8 and 1.26±0.2 mg/dL), creatinine (0.7±0.1 and 1.26±0.2 mg/dL), uric acid (1.6±0.16 and 1,56±0.08 mg/dL), bilirubin (0.43±0.02 and 0.3±0.05 mg/dL), AST (77.14±7.0 and 59±4.5 U/L), ALT (31.2±4.8 and 34.3±2.0 U/L), ALP (85±2.5 and 56.6±2.5 U/L), albumin (2.3±0.9 and 3.9±0.08 mg/dL), globulin (3.82±0.4 and 2.82±0.4 mg/dL), A/G ratio (1.01±0.6 and 2.0±0.5 %), total proteins (4.9±0 and 3.3±0.03 mg/dL) and HbA1C (3.7±0.89 and 3.9±0,01 %) in both male and female rats respectively while sodium (138.14±6.76 mmol/L), potassium (4.5±0.33 mmol/L), calcium (9.9±1.72 mg/dL), magnesium (1.7±0.44 mmol/L), chloride (106.50±8.5 mmol/L), amylase (443.79±29.28 U/L), lipase (18.6±4.1 U/L), LDH (688.86±13.54 U/L) were same in both genders, as compared to negative control [cholesterol (95.25±10.47 and 105±7.0 mg/dL), triglycerides (87.00±5.72 and 82.3±2.0 mg/dL), HDL (57.00±5.36 and 64.3±4.0 mg/dL), LDL (28.75±5.56 and 25.3±1.0 mg/dL), VLDL (13.25±1.71 and 12.6±0.5 U/L), Urea (80±6.0 and 67.5±3.8 mg/dL), creatinine (1.31±0.1 and 0.8±0.02 mg/dL), uric acid (1.6±0.10 ad 1.8±0.1 mg/dL), bilirubin (2.06±0.3 and 1.26±0.05 mg/dL), AST (199.83±8.3 and 155.5±5 U/L), ALT (72.3±5.6 and 77.1±4.8 U/L), ALP (95±3 and 89±6.3 U/L), albumin (2.9±0.5 and 3.36±0.04 mg/dL), globulin (1.03±0.03 and 2.4±0.2 mg/dL), total protein (6.56±0.7 and 6.64±2 mg/dL), A/G ratio (1.1±0.1 % in both), sodium (144.14±8.76 and 132.6±12 mmol/L), potassium (7.25±3.38 and 5.9±0.03 mmol/L), calcium (9.25±1.7 and 10.5±4.0 mmol/L), magnesium (4.01±0.448 and 2.5±0.2 mmol/L), chloride (101.50±0.58 and 106±10 mmol/L), amylase (312.6±10.28 and 418±13 U/L), lipase (180± 9.2 and 29.4±4.0 U/L), LDH (1654±164 and 1771±98 U/L) and HbA1c (4.4±0.29 and 3.2±0.4%)] while effect of quercetin extract was almost similar to positive control drug or metformin. Histopathological analysis showed recovery of renal, hepatic, and pancreatic tissues, along with a reduction in mononuclear cell infiltrate and improvement in steatosis in the liver, while pancreas showed mild restoration of pancreatic beta cells. Current results can be used for the isolation and hepato-renal safety profiling of quercetin from C. sinesnsis (L.) via its bioactivity guided isolation.