Developing Sciatic nerve compression model in rats using aneurysm clip and validating it by histological and behavior studies
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Keywords
Aneurysm Clip Compression, Sciatic Nerve Injury
Abstract
Aim: The aim of the study was to develop sciatic nerve compression model in rats using aneurysm
clip And to validate compression injury by histological examination and behavior testing
Study design and duration: experimental animal model
Methodology: The study included 16 healthy Sprague Dawley rats weighing 250 to 300 gm. The rats
were divided into two groups. Each group comprised of 4 male and 4 female rats. Sham group animals
were given only skin incision while in control group rats’ sciatic nerve injury was induced in right
limb under isoflurane anesthesia with an aneurysm clip having a force of 0.6 N. Animals were
observed on day 0, 1 and 7 for behavior changes
Results: On histological and behavior examination the animals showed marked changes in crush
injury group. On microscopy of tissue after H & E staining clearly evident injury was seen with
discontinuity in architecture of nerve and macrophage infiltration at the site of application of
aneurysm clamp. Animals in Injury group showed marked mechanical and thermal hyperalgesia and
cold allodynia as compared to sham group.
Conclusion: The evident histological alterations supported by the behavior changes in the injury
group confirm that the aneurysm clip model is a valid, self-effacing and easily reproducible method
to induce sciatic nerve injury in rats with uniform force application for experimental studies.
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Nazish Waheed
Associate Professor, Department of Anatomy, Jinnah Medical College, Peshawar, Pakistan
Zilli Huma
Associate Professor, Department of Anatomy, Khyber Medical University, Peshawar, Pakistan.
Noman Ullah Wazir
Associate Professor, Department of Anatomy, Peshawar Medical College, Peshawar, Pakistan
Fatima Daud
Assistant Professor, Department of Anatomy, Pak International Medical College, Peshawar, Pakistan.
Saima Mumtaz
Associate Professor, Department of Anatomy, Federal Medical College, Islamabad, Pakistan
Sara Ishaq
Assistant Professor, Department of Anatomy, Jinnah Medical College, Peshawar, Pakistan