IN VIVO TOXICITY COMPARISON STUDIES BETWEEN CAMPTOTHECIN AND DISULFIDE LINKED BIOTIN CONJUGATED CAMPTOTHECIN IN COLON TUMOR BEARING RATS
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Keywords
Antitumor, camptothecin, conjugation, biotin, toxicity
Abstract
Camptothecin is an important chemotherapeutic agent used in the treatment of several cancers and most commonly used as first line drug in treatment of colon cancer. However, it has several side effects including nephrotoxicity, hepatotoxicity, and ototoxicity. In in vitro experiments, several vitamins have shown antitumor protective effective against toxicity produced by chemotherapeutic drugs. Biotin is one such bioactive vitamin and various authors have reported that it has strong antioxidant and antitumor potential when used in conjugation with antitumor drugs. The present study was, therefore, carried out to explore the protective potential of CPT-SS-Biotin on DMH-induced hepatotoxicity and nephrotoxicity in colon tumor-bearing rats. Animals were divided into four groups: Group I: normal control, Group II: DMH treated, Group III: DMH+ CPT-SS-Biotin treated and Group IV: DMH+ standard camptothecin. Administration of conjugated CPT significantly ameliorated the toxicity caused by DMH as indicated by improved liver function tests, kidney function tests and hematological tests more efficiently in comparison to CPT alone. The same was also evident from the improvement in the histopathological changes in kidney and testis. Blood counts were also improved on administration of conjugate to DMH-treated rats. This article provides the evidence that antioxidant efficacy of biotin has beneficial effects on DMH-induced nephrotoxicity and hepatotoxicity.
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Amardeep Kaur
College of Pharmacy, M.M. Deemed to be University, Mullana-133207 Haryana India
Shikha Dhiman
College of Pharmacy, M.M. Deemed to be University, Mullana-133207 Haryana India
Manu Sharma
National Forensic Sciences University, Sector-3 Rohini-110085 New Delhi India;
+91-9882605005