PREVALENCE OF ALBICANS CANDIDA AND NON-ALBICANS CANDIDA IN NEONATES ADMITTED FOR SEPTICEMIA WITH ASSOCIATED RISK FACTORS
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Keywords
Low birth weight, Prematurity, Colonization, Parentral nutrition, Horizontal transmission
Abstract
Aim: To know the prevalence and epidemiology of candidemia an uncommon infection among newborns admitted to our hospital.
-To summarize the clinical characteristics of candidemia in neonates, and determine whether specific risk factors may be identified to aid the selection of patients.
Materials and Methods: This prospective study was conducted between August 2018 and May 2019 in the Departments of Microbiology and Pediatrics of tertiary care hospital in central , India. From a total 244 neonates admitted to NICU with clinical suspicion of septicaemia. blood samples were collected in blood culture bottle and incubated at 370C followed by subculture done on blood agar and Mc conkey agar and Sabouraud’s dextrose agar with chloramphenicol (0.05%) and incubated at 370C. Only those isolates which yielded pure growth of candida spp. Were included in the study. Preliminary identification was done by colony morphology on SDA , Chromogenic medium, growth at 450C , Germ Tube test , chlamydospore formation and was confirmed by carbohydrate fermentation test. Candidemia was defiend as the presence of at least one positive blood culture containing pure growth of candida spp. With supportive clinical feature.
Results- Total 244 neonates included in the study, 129(53%)were females and 115(47%) were males .Candida albicans responsible for 64% cases while NAC species responsible for 36% cases with14% cases by C. tropicalis, 08% cases by C. parapsillosis, 7% by C. gullermondii, 4% by C. krusei, 3% by C.kefyr. The risk factors observed for candedimia are prematurity and LBW , indwelling catheters are commonest followed by broad spectrum antibiotic use, TPN prolonged hyperalimentation. Various clinical presentations also seen in Candidemia.
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