STABILITY INDICATING HPTLC METHOD FOR ESTIMATION  OF LOPINAVIR AND RITONAVIR IN FIXED-DOSE COMBINATION TABLETS.

Dr.G.Dharmamoorthy; S.Roopanvitha; K.Harshitha; Y.Krupananda; A.Tharunkumar5; Dr.Mallikarjuna B.P; Dr.M.Pradeep

doi:10.53555/jptcp.v30i15.2563

Dr.G.Dharmamoorthy
S.Roopanvitha
K.Harshitha
Y.Krupananda
A.Tharunkumar5
Dr.Mallikarjuna B.P
Dr.M.Pradeep

Keywords

Lopinavir(LPV) and Ritonavir(RTV), HPTLC, Forced degradation, Validation.

Abstract

A stability indicating simple, precise,accurate,rapid and validated HPTLC method for simultaneous estimation of Lopinavir and Ritanavir was successfully developed. The method is based on HPTLC separation of the two drugs followed by densitometric measurements of their spots at 266 nm.This method is based on HPTLC separation followed by UV detection at 266 nm. The separation was carried out on merck TLC aluminium sheets precoated with silica gel 60 F₂₅₄ using a camag Linomat5 The mobile consists of Benzene: ethanol : aceticacid (7:3: 0.4% v/v) . Calibration curves were linear in range of 800-4800 ng/band and 200- 1200 ng/ band Lopinavir and Ritonavir respectively . LPV and RTV gave sharp and well defined peaks at Rf value are 0.63 and 0.44 respectively, Stress degradation study shows that sample degraded with acid and base hydrolysis, under oxidation, thermal and photolytic stress conditions.. No chromatographic interferences from the tablet excipients were found. The method was validated in accordance with the requirements of ICH guidelines.

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References

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Published

Aug 17, 2023

DOI: https://doi.org/10.53555/jptcp.v30i15.2563

How to Cite

Dr.G.Dharmamoorthy, S.Roopanvitha, K.Harshitha, Y.Krupananda, A.Tharunkumar5, Dr.Mallikarjuna B.P, & Dr.M.Pradeep. (2023). STABILITY INDICATING HPTLC METHOD FOR ESTIMATION OF LOPINAVIR AND RITONAVIR IN FIXED-DOSE COMBINATION TABLETS. Journal of Population Therapeutics and Clinical Pharmacology, 30(15), 527-540. https://doi.org/10.53555/jptcp.v30i15.2563

Issue

Vol. 30 No. 15 (2023): JPTCP

Section

Articles

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Author Biographies

Dr.G.Dharmamoorthy

Professor &HOD Department of Pharmaceutical Analysis. MB School of Pharmaceutical Sciennces (Erst while Sree Vidyanikethan College of Pharmacy) Mohan Babu University.Sree Sainath Nagar.Rangampeta Tirupathi-517102 Andhrapradesh, Mobile no; +91 9603774847,