A COMPARATIVE STUDY OF GCMS ANALYSIS OF BIOACTIVE COMPOUND ISOLATED FROM MARINE ALGAE-DERIVED ENDOPHYTIC FUNGI
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Keywords
Endophytic fungi, phytoconstituents, GCMS analysis, chromatogram, therapeutic value, 18 S RNA sequences
Abstract
The present investigation was focused to screen the phytochemical constituents of ethyl acetate extract of endophytic fungi isolated from seven different types of green algae and brown algae. These isolated endophytic fungi were purified and identified by 18 S RNA sequencing and were deposited in NCBI. The identified organism was subjected for bioactive extraction using ethyl acetate. The bioactive compounds obtained from five different fungi were analyzed using GCMS and the mass spectrum of each compound was matched with NIST and Wiley 8 Library. Totally 66 compounds were identified during the period of study out of which 26 phytocompounds were from Curvilariaplatzii, 14 from Chaetomium perlucidum, 15 from Corynascussepedonium, 11 from Talaromycesaurantiacu and 4 from Scopulariopsisgracilis, respectively. The major compounds registered during the period of study were Pyrrolo[1,2-a]pyrazine-1,4-dione, Hexahydro-3-(2-methylprop; Cyclo(L-prolyl-L-valine); (2S,6R)-2,6-Dibutyl-4-Methylpiperidine, Phthalic acid, 5- methylhex-2-yl butyl ester; Phthalic acid, Hex-3-yl isobutyl ester,Bis (2- ethyl methyl phthalate) , Octadecane,3 ethyl 5 (2- ethyl butyl) and Pyrole,17- pentatriocontene. Out of five fungal organisms, Curvilariaplatzii showed a large number of bioactive compounds in ethyl acetate extraction and they are of high therapeutic value in the medical field and has various industrial applications.
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A K Ramya
Department of Microbiology, Aarupadai Veedu Medical College and Hospital, VMRF, Pondicherry, India
Dr R Devika
Department of Biotechnology, Aarupadai Veedu Institute of Technology, Paiyanoor, Chennai, India
Dr K Sethumadhavan
Department of Microbiology, Aarupadai Veedu Medical College and Hospital, VMRF, Pondicherry, India