EFFECT OF BETA- SITOSTEROL ON EXPRESSION OF TGF- β, IGF-1 AND TNF-α mRNA IN LPS INDUCED FIBROBLAST CELL LINE- A MOLECULAR STUDY
Main Article Content
Keywords
Transforming growth factor-beta (TGF-β), Tumour Necrosis Factor–α (TNF-α), lipopolysaccharide (LPS), Insulin Growth Factor (IGF-1), Beta Sitosterol, Fibroblast, medical
Abstract
Introduction
The lipopolysaccharides(LPS) found in the gram negative bacteria is one of the important factors for the inflammation of pulp and periapical tissues. LPS is reported to increase inflammation by increasing TNF-α and downregulates growth factors like TGF-β and IGF-1. Phytosterols have a prominent anti-inflammatory function and wound healing capacity. The Aim of the study was to determine the effect of Beta Sitosterol on the expression of TGF-β, IGF-1, and TNF-α mRNA in LPS treated fibroblast cell lines.
Results
LPS induced fibroblast cultures showed an increase in the expression of TNF-α mRNA and reduced expression of TGF-β and IGF-1 mRNA. Treatment with Beta Sitosterol reduced the expression of TNF-α mRNA and increased the expression of TGF-β and IGF-1 mRNA in LPS induced fibroblast cell culture.
Conclusion
Beta sitosterol increased the expression of growth factors and downregulated the inflammatory factors thus potentiating its use in inflammatory conditions. It can be effectively used as intracanal medicament or pulp capping agent. Nevertheless, there is a need for controlled trials to establish its effectiveness clinically.
References
1. Carthy JM. TGFβ signaling and the control of myofibroblast differentiation: Implications for chronic inflammatory disorders [Internet]. Vol. 233, Journal of Cellular Physiology. 2018. p. 98–
106. Available from: http://dx.doi.org/10.1002/jcp.25879
2. Kubiczkova L, Sedlarikova L, Hajek R, Sevcikova S. TGF-β – an excellent servant but a bad master [Internet]. Vol. 10, Journal of Translational Medicine. 2012. p. 183. Available from: http://dx.doi.org/10.1186/1479-5876-10-183
3. Brown LR, Rudolph CE. Isolation and identification of microorganisms from unexposed canals of pulp-involved teeth [Internet]. Vol. 10, Oral Surgery, Oral Medicine, Oral Pathology. 1957. p. 1094–9. Available from: http://dx.doi.org/10.1016/0030-4220(57)90061-0
4. Kantz WE, Henry CA. Isolation and classification of anaerobic bacteria from intact pulp chambers of non-vital teeth in man [Internet]. Vol. 19, Archives of Oral Biology. 1974. p. 91–6. Available from: http://dx.doi.org/10.1016/0003-9969(74)90231-3
5. Bradley JR. TNF-mediated inflammatory disease [Internet]. Vol. 214, The Journal of Pathology. 2008. p. 149–60. Available from: http://dx.doi.org/10.1002/path.2287
6. Voigt J. Physiology and Biochemistry of Sterols. Ed. by G. W. Patterson and W. D. Nes. 395 pages, numerous tables. American Oil Chemists’ Society, Champaign, Illinois, 1991. Price: 80,– (for members 60,– ) [Internet]. Vol. 36, Food / Nahrung. 1992. p. 424–424. Available from: http://dx.doi.org/10.1002/food.19920360437
7. Bouic PJ, Clark A, Lamprecht J, Freestone M, Pool EJ, Liebenberg RW, et al. The effects of B- sitosterol (BSS) and B-sitosterol glucoside (BSSG) mixture on selected immune parameters of marathon runners: inhibition of post marathon immune suppression and inflammation. Int J Sports Med. 1999 May;20(4):258–62.
8. Khan A, Rahman M, Islam S. Antipyretic Activity of Peperomia pellucida Leaves in Rabbit. Turk J Biol. 2008 Feb 19;32(1):37–41.
9. Zeb MA. Isolation and Biological Activity of β-Sitosterol and Stigmasterol from the Roots of Indigofera heterantha [Internet]. Vol. 5, Pharmacy & Pharmacology International Journal. 2017. Available from: http://dx.doi.org/10.15406/ppij.2017.05.00139
10. Panigrahi J, Gantait S, Patel IC. Concurrent production and relative quantification of vasicinone from in vivo and in vitro plant parts of Malabar nut (Adhatoda vasica Nees) [Internet]. Vol. 7, 3 Biotech. 2017. Available from: http://dx.doi.org/10.1007/s13205-017-0882-7
11. Website.
12. Sundqvist G. Ecology of the root canal flora [Internet]. Vol. 18, Journal of Endodontics. 1992. p. 427–30. Available from: http://dx.doi.org/10.1016/s0099-2399(06)80842-3
13. Barthel CR, Levin LG, Reisner HM, Trope M. TNF-alpha release in monocytes after exposure to calcium hydroxide treated Escherichia coli LPS. Int Endod J. 1997 May;30(3):155–9.
14. Seltzer S, Farber PA. Microbiologic factors in endodontology. Oral Surg Oral Med Oral Pathol. 1994 Nov;78(5):634–45.
15. Morrison DC, Ryan JL. Mechanisms [Internet]. Vol. 38, Annual Review of Medicine. 1987. p. 417–32. Available from: http://dx.doi.org/10.1146/annurev.me.38.020187.002221
16. Rhodes JS. Advanced Endodontics: Clinical Retreatment and Surgery. CRC Press; 2005. 218 p.
17. Antony D, Subramanian A, Nivedhitha M, Solete P, Balasubramaniam A. Post-endodontic pain with different engine-driven endodontic instruments in multi-visit root canal therapy: A systematic review and meta-analysis. J Int Oral Health. 2022;14(1):1.
18. Wolff SM. Biological Effects of Bacterial Endotoxins in Man [Internet]. Vol. 128, Journal of Infectious Diseases. 1973. p. S259–64. Available from: http://dx.doi.org/10.1093/infdis/128.supplement_1.s259
19. Rhodes JS. Advanced Endodontics: Clinical Retreatment and Surgery. CRC Press; 2005. 218 p.
20. S DPA, Solete P, Jeevanandan G, Syed AA, Almahdi S, Alzhrani M, et al. Effect of Various Irrigant Activation Methods and Its Penetration in the Apical Third of Root Canal-In Vitro Study. Eur J Dent. 2023 Feb;17(1):57–61.
21. Chu LH, Indramohan M, Ratsimandresy RA, Gangopadhyay A, Morris EP, Monack DM, et al. The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages. Nat Commun. 2018 Mar 8;9(1):996.
22. Raetz CRH, Whitfield C. Lipopolysaccharide Endotoxins [Internet]. Vol. 71, Annual Review of Biochemistry. 2002. p. 635–700. Available from: http://dx.doi.org/10.1146/annurev.biochem.71.110601.135414
23. Raetz CR. Bacterial endotoxins: extraordinary lipids that activate eucaryotic signal transduction [Internet]. Vol. 175, Journal of Bacteriology. 1993. p. 5745–53. Available from: http://dx.doi.org/10.1128/jb.175.18.5745-5753.1993
24. Munford RS, Hall CL. Detoxification of bacterial lipopolysaccharides (endotoxins) by a human neutrophil enzyme. Science. 1986 Oct 10;234(4773):203–5.
25. Brady TA, Piesco NP, Buckley MJ, Langkamp HH, Bowen LL, Agarwal S. Autoregulation of Periodontal Ligament Cell Phenotype and Functions by Transforming Growth Factor-β1 [Internet]. Vol. 77, Journal of Dental Research. 1998. p. 1779–90. Available from: http://dx.doi.org/10.1177/00220345980770100501
26. Matsushita K, Tajima T, Tomita K, Takada H, Nagaoka S, Torii M. Inflammatory cytokine production and specific antibody responses to lipopolysaccharide from endodontopathic black- pigmented bacteria in patients with multilesional periapical periodontitis. J Endod. 1999 Dec;25(12):795–9.
27. Damjanov I. Book Review Oxford Textbook of Pathology Edited by James O. McGee, Peter G. Isaacson, and Nicholas A. Wright. 2344 pp. in three volumes, illustrated. New York, Oxford University Press, 1992. $295. 0-19-261976-4 [Internet]. Vol. 328, New England Journal of Medicine. 1993. p. 1503–1503. Available from: http://dx.doi.org/10.1056/nejm199305203282024
28. Silva TA, Garlet GP, Lara VS, Martins W Jr, Silva JS, Cunha FQ. Differential expression of chemokines and chemokine receptors in inflammatory periapical diseases. Oral Microbiol Immunol. 2005 Oct;20(5):310–6.
29. Prso IB, Kocjan W, Simić H, Brumini G, Pezelj-Ribarić S, Borcić J, et al. Tumor necrosis factor- alpha and interleukin 6 in human periapical lesions. Mediators Inflamm. 2007;2007:38210.
30. Khayat B, Jouanny G. Microsurgical Endodontics. Quintessence Publishing (IL); 2019.
31. Ma J, Al-Ashaw AJ, Shen Y, Gao Y, Yang Y, Zhang C, et al. Efficacy of ProTaper Universal Rotary Retreatment system for gutta-percha removal from oval root canals: a micro-computed tomography study. J Endod. 2012 Nov;38(11):1516–20.
32. Indi S, Desai SR, Hambire A, Mustafa M, Almokhatieb AA, Abuelqomsan MAS, et al. Comparison of the Time Required by Six Different Retreatment Techniques for Retrieval of Gutta-Percha: An In Vitro Study [Internet]. European Journal of General Dentistry. 2022. Available from: http://dx.doi.org/10.1055/s-0042-1750089
33. Siddique R, Nivedhitha MS, Ranjan M, Jacob B, Solete P. Comparison of antibacterial effectiveness of three rotary file system with different geometry in infected root canals before and after instrumentation-a double-blinded randomized controlled clinical trial. BDJ Open. 2020 Jun 8;6:8.
34. Siddique R, Nivedhitha MS. Effectiveness of rotary and reciprocating systems on microbial reduction: A systematic review. J Conserv Dent. 2019 Mar-Apr;22(2):114–22.
35. Letterio JJ, Roberts AB. Transforming growth factor-β1-deficient mice: identification of isoform- specific activities in vivo [Internet]. Vol. 59, Journal of Leukocyte Biology. 1996. p. 769–74. Available from: http://dx.doi.org/10.1002/jlb.59.6.769
36. Khayat B, Jouanny G. Microsurgical Endodontics. Quintessence Publishing (IL); 2019.
37. Pratheebha C, Gayathri R, Veeraraghavan VP, Kavitha S. Knowledge, awareness, and perception on root canal treatment among South Indian population - A survey. J Adv Pharm Technol Res. 2022 Nov;13(Suppl 1):S302–7.
38. Joyce ME, Roberts AB, Sporn MB, Bolander ME. Transforming growth factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur [Internet]. Vol. 110, Journal of Cell Biology. 1990. p. 2195–207. Available from: http://dx.doi.org/10.1083/jcb.110.6.2195
39. Waris V, Xu JW, Nordsletten L, Sorsa T, Santavirta S, Konttinen YT. Basic Fibroblast Growth Factor (bFGF) in the Synovial-like Membrane around Loose Total Hip Prostheses [Internet]. Vol. 25, Scandinavian Journal of Rheumatology. 1996. p. 257–62. Available from: http://dx.doi.org/10.3109/03009749609069995
40. Crane JL, Zhao L, Frye JS, Xian L, Qiu T, Cao X. IGF-1 Signaling is Essential for Differentiation of Mesenchymal Stem Cells for Peak Bone Mass [Internet]. Vol. 1, Bone Research. 2013. p. 186–
94. Available from: http://dx.doi.org/10.4248/br201302007
41. Fujita T, Azuma Y, Fukuyama R, Hattori Y, Yoshida C, Koida M, et al. Runx2 induces osteoblast and chondrocyte differentiation and enhances their migration by coupling with PI3K-Akt signaling [Internet]. Vol. 166, Journal of Cell Biology. 2004. p. 85–95. Available from: http://dx.doi.org/10.1083/jcb.200401138
42. Xi G, Shen X, Rosen CJ, Clemmons DR. IRS-1 Functions as a Molecular Scaffold to Coordinate IGF-I/IGFBP-2 Signaling During Osteoblast Differentiation [Internet]. Vol. 31, Journal of Bone
and Mineral Research. 2016. p. 1300–14. Available from: http://dx.doi.org/10.1002/jbmr.2791
43. Nasim I, Professor and Head, Department of Conservative Dentistry and Endodontics, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai - 600077, India. Effect of nanoparticles based root canal disinfectants on Enterococcus faecalis - A systematic review. Int J Dent Oral Sci. 2021 Jun 30;2898–904.
44. Roman-Blas JA, Stokes DG, Jimenez SA. Modulation of TGF-β signaling by proinflammatory cytokines in articular chondrocytes [Internet]. Vol. 15, Osteoarthritis and Cartilage. 2007. p. 1367–77. Available from: http://dx.doi.org/10.1016/j.joca.2007.04.011
45. Evaluation of an Ultrasonic Technique to Remove Fractured Rotary Nickel-Titanium Endodontic Instruments from Root Canals: Clinical Cases. J Endod. 2003 Nov 1;29(11):764–7.
46. Natanasabapathy V, Durvasulu A, Krithikadatta J, Namasivayam A, Deivanayagam K, Manali S, et al. Current Trends in the Use of Irrigant Activation Techniques Among Endodontists & Post-Graduate Dental Students in India -A Knowledge, Attitude and Practice Based Survey. Eur Endod J. 2020 May 22;5(2):73–80.
47. Mitchell K, Shah JP, Tsytsikova LV, Campbell AM, Affram K, Symes AJ. LPS antagonism of TGF-β signaling results in prolonged survival and activation of rat primary microglia. J Neurochem. 2014 Apr;129(1):155–68.
48. Kulkarni S, Wahane K, Daokar S, Patil K, Patel K, Thorat T. An assessment of the efficacy of a rotary and a reciprocating retreatment file system for removal of gutta-percha from root canals: An in vitro cone-beam computed tomography study. Endodontology. 2021;33(1):20.
49. Azim AA, Wang HH, Tarrosh M, Azim KA, Piasecki L. Comparison between Single-file Rotary Systems: Part 1—Efficiency, Effectiveness, and Adverse Effects in Endodontic Retreatment [Internet]. Vol. 44, Journal of Endodontics. 2018. p. 1720–4. Available from: http://dx.doi.org/10.1016/j.joen.2018.07.022
50. Rodrigues CT, Duarte MAH, de Almeida MM, de Andrade FB, Bernardineli N. Efficacy of CM- Wire, M-Wire, and Nickel-Titanium Instruments for Removing Filling Material from Curved Root Canals: A Micro–Computed Tomography Study [Internet]. Vol. 42, Journal of Endodontics. 2016. p. 1651–5. Available from: http://dx.doi.org/10.1016/j.joen.2016.08.012
51. Divya S, Jeevanandan G, Sujatha S, Subramanian EMG, Ravindran V. Comparison of quality of obturation and post-operative pain using manual vs rotary files in primary teeth - A randomised clinical trial. Indian J Dent Res. 2019 Nov-Dec;30(6):904–8.
52. Briard N, Dadoun F, Pommier G, Sauze N, Lebouc Y, Oliver C, et al. IGF-I/IGFBPs system response to endotoxin challenge in sheep. J Endocrinol. 2000 Mar;164(3):361–9.
53. Endodontic retreatment—Case selection and technique. Part 2: Treatment planning for retreatment. J Endod. 1988 Dec 1;14(12):607–14.
54. A statistical analysis of surgical and nonsurgical endodontic retreatment cases. J Endod. 1989 Jun 1;15(6):261–6.
55. Results of endodontic retreatment: A randomized clinical study comparing surgical and nonsurgical procedures. J Endod. 1999 Dec 1;25(12):814–7.
56. Panchal V, Gurunathan D, Muralidharan NP. Comparison of antibacterial efficacy of cinnamon extract, neem extract as irrigant and sodium hypochlorite against : An study. Indian J Dent Res. 2020 Jan-Feb;31(1):124–8.
57. Fan J, Molina PE, Gelato MC, Lang CH. Differential tissue regulation of insulin-like growth factor-I content and binding proteins after endotoxin [Internet]. Vol. 134, Endocrinology. 1994. p. 1685–92. Available from: http://dx.doi.org/10.1210/endo.134.4.7511091
58. Soto L, Martin AI, Millan S, Vara E, Lopez-Calderon A. Effects of endotoxin lipopolysaccharide administration on the somatotropic axis [Internet]. Vol. 159, Journal of Endocrinology. 1998. p. 239–46. Available from: http://dx.doi.org/10.1677/joe.0.1590239
59. Chang CH, Wen ZH, Wang SK, Duh CY. New anti-inflammatory steroids from the Formosan soft coral Clavularia viridis [Internet]. Vol. 73, Steroids. 2008. p. 562–7. Available from: http://dx.doi.org/10.1016/j.steroids.2008.01.007
60. Fernando IPS, Shanura Fernando IP, Asanka Sanjeewa KK, Kim HS, Kim SY, Lee SH, et al. Identification of sterols from the soft coral Dendronephthya gigantea and their anti-inflammatory potential [Internet]. Vol. 55, Environmental Toxicology and Pharmacology. 2017. p. 37–43. Available from: http://dx.doi.org/10.1016/j.etap.2017.08.003
61. Kobori M. Anti-inflammatory, Anti-cancer and Anti-diabetic Properties of Sterols and Polyphenols in Vegetables and Fruits [Internet]. Vol. 56, Nippon Shokuhin Kagaku Kogaku Kaishi. 2009. p. 614–9. Available from: http://dx.doi.org/10.3136/nskkk.56.614
62. Bhagavaldas MC, Diwan A, Kusumvalli S, Pasha S, Devale M, Chava DC. Efficacy of two rotary retreatment systems in removing Gutta-percha and sealer during endodontic retreatment with or without solvent: A comparative study. J Conserv Dent. 2017 Jan-Feb;20(1):12–6.
63. Sundar S, Varghese A, Datta KJ, Natanasabapathy V. Effect of guided conservative endodontic access and different file kinematics on debris extrusion in mesial root of the mandibular molars: An study. J Conserv Dent. 2022 Sep 12;25(5):547–54.
64. Hu J, Yang B, Lin X, Zhou X, Yang X, Long L, et al. Chemical and Biological Studies of Soft Corals of the Nephtheidae Family [Internet]. Vol. 8, Chemistry & Biodiversity. 2011. p. 1011–
32. Available from: http://dx.doi.org/10.1002/cbdv.201000105
65. Huang YC, Wen ZH, Wang SK, Hsu CH, Duh CY. New anti-inflammatory 4-methylated steroids from the Formosan soft coral Nephthea chabroli [Internet]. Vol. 73, Steroids. 2008. p. 1181–6. Available from: http://dx.doi.org/10.1016/j.steroids.2008.05.007
66. Khayat B, Jouanny G. Microsurgical Endodontics. Quintessence Publishing (IL); 2019.
67. Ma J, Al-Ashaw AJ, Shen Y, Gao Y, Yang Y, Zhang C, et al. Efficacy of ProTaper Universal Rotary Retreatment system for gutta-percha removal from oval root canals: a micro-computed tomography study. J Endod. 2012 Nov;38(11):1516–20.
68. Indi S, Desai SR, Hambire A, Mustafa M, Almokhatieb AA, Abuelqomsan MAS, et al. Comparison of the Time Required by Six Different Retreatment Techniques for Retrieval of Gutta-Percha: An In Vitro Study [Internet]. European Journal of General Dentistry. 2022. Available from: http://dx.doi.org/10.1055/s-0042-1750089
69. Wong R. Conventional endodontic failure and retreatment. Dent Clin North Am. 2004 Jan;48(1):265–89.
70. Nivedhitha N, Professor and Head, Department of Conservative Dentistry and Endodontics, Saveetha Dental college and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India. Comparing the effectiveness of various irrigant activation techniques with conventional needle irrigation - A systematic review. Int J Dent Oral Sci. 2021 May 30;2626–31.
71. Siddique R, Nivedhitha MS, Jacob B. Quantitative analysis for detection of toxic elements in various irrigants, their combination (precipitate), and para-chloroaniline: An inductively coupled plasma mass spectrometry study. J Conserv Dent. 2019 Jul-Aug;22(4):344–50.
72. Paz SM de la, la Paz SM de, Fernández-Arche Á, Ángel-Martín M, García-Giménez MD. The sterols isolated from Evening Primrose oil modulate the release of proinflammatory mediators [Internet]. Vol. 19, Phytomedicine. 2012. p. 1072–6. Available from: http://dx.doi.org/10.1016/j.phymed.2012.06.008
73. Identification of Sitosterol as in Vitro Anti-Inflammatory Constituent in Moringa oleifera [Internet]. Available from: http://dx.doi.org/10.1021/acs.jafc.8b04555.s001
74. Sagare SV, Chandra P, Kaur T, Ganorkar O, Khade A, Mehta SD. A comparative study of the efficacy of WaveOne and NeoEndo retreatment file system for the removal of Gutta percha from the root canal. J Pharm Bioallied Sci. 2021 Nov;13(Suppl 2):S1682–5.
75. Hima Sandeep A, Senior Lecturer, Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences. Prevalence and associated factors of root canal treated mandibular anterior teeth with and without post endodontic crowns. Int J Dent Oral Sci. 2019 Nov 30;87–91.
76. Choi JN, Choi YH, Lee JM, Noh IC, Park JW, Choi WS, et al. Anti-inflammatory effects of β-
sitosterol-β-D-glucoside from Trachelospermum jasminoides (Apocynaceae) in lipopolysaccharide-stimulated RAW 264.7 murine macrophages. Nat Prod Res. 2012 Jan 31;26(24):2340–3.
77. Kassen A, Berges R, Senge T. Effect of Beta–Sitosterol on Transforming Growth Factor–Beta– 1 Expression and Translocation Protein Kinase C Alpha in Human Prostate Stromal Cells in vitro [Internet]. Vol. 37, European Urology. 2000. p. 735–41. Available from: http://dx.doi.org/10.1159/000020227
78. Kulkarni S, Wahane K, Daokar S, Patil K, Patel K, Thorat T. An assessment of the efficacy of a rotary and a reciprocating retreatment file system for removal of gutta-percha from root canals: An in vitro cone-beam computed tomography study. Endodontology. 2021;33(1):20.
79. Azim AA, Wang HH, Tarrosh M, Azim KA, Piasecki L. Comparison between Single-file Rotary Systems: Part 1—Efficiency, Effectiveness, and Adverse Effects in Endodontic Retreatment [Internet]. Vol. 44, Journal of Endodontics. 2018. p. 1720–4. Available from: http://dx.doi.org/10.1016/j.joen.2018.07.022
80. Sagare SV, Chandra P, Kaur T, Ganorkar O, Khade A, Mehta SD. A comparative study of the efficacy of WaveOne and NeoEndo retreatment file system for the removal of Gutta percha from the root canal. J Pharm Bioallied Sci. 2021 Nov;13(Suppl 2):S1682–5.
81. Janani K, Teja KV, Ajitha P, Sandhya R. Evaluation of tissue inflammatory response of four intracanal medicament - An animal study. J Conserv Dent. 2020 Dec 4;23(3):216–20.
106. Available from: http://dx.doi.org/10.1002/jcp.25879
2. Kubiczkova L, Sedlarikova L, Hajek R, Sevcikova S. TGF-β – an excellent servant but a bad master [Internet]. Vol. 10, Journal of Translational Medicine. 2012. p. 183. Available from: http://dx.doi.org/10.1186/1479-5876-10-183
3. Brown LR, Rudolph CE. Isolation and identification of microorganisms from unexposed canals of pulp-involved teeth [Internet]. Vol. 10, Oral Surgery, Oral Medicine, Oral Pathology. 1957. p. 1094–9. Available from: http://dx.doi.org/10.1016/0030-4220(57)90061-0
4. Kantz WE, Henry CA. Isolation and classification of anaerobic bacteria from intact pulp chambers of non-vital teeth in man [Internet]. Vol. 19, Archives of Oral Biology. 1974. p. 91–6. Available from: http://dx.doi.org/10.1016/0003-9969(74)90231-3
5. Bradley JR. TNF-mediated inflammatory disease [Internet]. Vol. 214, The Journal of Pathology. 2008. p. 149–60. Available from: http://dx.doi.org/10.1002/path.2287
6. Voigt J. Physiology and Biochemistry of Sterols. Ed. by G. W. Patterson and W. D. Nes. 395 pages, numerous tables. American Oil Chemists’ Society, Champaign, Illinois, 1991. Price: 80,– (for members 60,– ) [Internet]. Vol. 36, Food / Nahrung. 1992. p. 424–424. Available from: http://dx.doi.org/10.1002/food.19920360437
7. Bouic PJ, Clark A, Lamprecht J, Freestone M, Pool EJ, Liebenberg RW, et al. The effects of B- sitosterol (BSS) and B-sitosterol glucoside (BSSG) mixture on selected immune parameters of marathon runners: inhibition of post marathon immune suppression and inflammation. Int J Sports Med. 1999 May;20(4):258–62.
8. Khan A, Rahman M, Islam S. Antipyretic Activity of Peperomia pellucida Leaves in Rabbit. Turk J Biol. 2008 Feb 19;32(1):37–41.
9. Zeb MA. Isolation and Biological Activity of β-Sitosterol and Stigmasterol from the Roots of Indigofera heterantha [Internet]. Vol. 5, Pharmacy & Pharmacology International Journal. 2017. Available from: http://dx.doi.org/10.15406/ppij.2017.05.00139
10. Panigrahi J, Gantait S, Patel IC. Concurrent production and relative quantification of vasicinone from in vivo and in vitro plant parts of Malabar nut (Adhatoda vasica Nees) [Internet]. Vol. 7, 3 Biotech. 2017. Available from: http://dx.doi.org/10.1007/s13205-017-0882-7
11. Website.
12. Sundqvist G. Ecology of the root canal flora [Internet]. Vol. 18, Journal of Endodontics. 1992. p. 427–30. Available from: http://dx.doi.org/10.1016/s0099-2399(06)80842-3
13. Barthel CR, Levin LG, Reisner HM, Trope M. TNF-alpha release in monocytes after exposure to calcium hydroxide treated Escherichia coli LPS. Int Endod J. 1997 May;30(3):155–9.
14. Seltzer S, Farber PA. Microbiologic factors in endodontology. Oral Surg Oral Med Oral Pathol. 1994 Nov;78(5):634–45.
15. Morrison DC, Ryan JL. Mechanisms [Internet]. Vol. 38, Annual Review of Medicine. 1987. p. 417–32. Available from: http://dx.doi.org/10.1146/annurev.me.38.020187.002221
16. Rhodes JS. Advanced Endodontics: Clinical Retreatment and Surgery. CRC Press; 2005. 218 p.
17. Antony D, Subramanian A, Nivedhitha M, Solete P, Balasubramaniam A. Post-endodontic pain with different engine-driven endodontic instruments in multi-visit root canal therapy: A systematic review and meta-analysis. J Int Oral Health. 2022;14(1):1.
18. Wolff SM. Biological Effects of Bacterial Endotoxins in Man [Internet]. Vol. 128, Journal of Infectious Diseases. 1973. p. S259–64. Available from: http://dx.doi.org/10.1093/infdis/128.supplement_1.s259
19. Rhodes JS. Advanced Endodontics: Clinical Retreatment and Surgery. CRC Press; 2005. 218 p.
20. S DPA, Solete P, Jeevanandan G, Syed AA, Almahdi S, Alzhrani M, et al. Effect of Various Irrigant Activation Methods and Its Penetration in the Apical Third of Root Canal-In Vitro Study. Eur J Dent. 2023 Feb;17(1):57–61.
21. Chu LH, Indramohan M, Ratsimandresy RA, Gangopadhyay A, Morris EP, Monack DM, et al. The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages. Nat Commun. 2018 Mar 8;9(1):996.
22. Raetz CRH, Whitfield C. Lipopolysaccharide Endotoxins [Internet]. Vol. 71, Annual Review of Biochemistry. 2002. p. 635–700. Available from: http://dx.doi.org/10.1146/annurev.biochem.71.110601.135414
23. Raetz CR. Bacterial endotoxins: extraordinary lipids that activate eucaryotic signal transduction [Internet]. Vol. 175, Journal of Bacteriology. 1993. p. 5745–53. Available from: http://dx.doi.org/10.1128/jb.175.18.5745-5753.1993
24. Munford RS, Hall CL. Detoxification of bacterial lipopolysaccharides (endotoxins) by a human neutrophil enzyme. Science. 1986 Oct 10;234(4773):203–5.
25. Brady TA, Piesco NP, Buckley MJ, Langkamp HH, Bowen LL, Agarwal S. Autoregulation of Periodontal Ligament Cell Phenotype and Functions by Transforming Growth Factor-β1 [Internet]. Vol. 77, Journal of Dental Research. 1998. p. 1779–90. Available from: http://dx.doi.org/10.1177/00220345980770100501
26. Matsushita K, Tajima T, Tomita K, Takada H, Nagaoka S, Torii M. Inflammatory cytokine production and specific antibody responses to lipopolysaccharide from endodontopathic black- pigmented bacteria in patients with multilesional periapical periodontitis. J Endod. 1999 Dec;25(12):795–9.
27. Damjanov I. Book Review Oxford Textbook of Pathology Edited by James O. McGee, Peter G. Isaacson, and Nicholas A. Wright. 2344 pp. in three volumes, illustrated. New York, Oxford University Press, 1992. $295. 0-19-261976-4 [Internet]. Vol. 328, New England Journal of Medicine. 1993. p. 1503–1503. Available from: http://dx.doi.org/10.1056/nejm199305203282024
28. Silva TA, Garlet GP, Lara VS, Martins W Jr, Silva JS, Cunha FQ. Differential expression of chemokines and chemokine receptors in inflammatory periapical diseases. Oral Microbiol Immunol. 2005 Oct;20(5):310–6.
29. Prso IB, Kocjan W, Simić H, Brumini G, Pezelj-Ribarić S, Borcić J, et al. Tumor necrosis factor- alpha and interleukin 6 in human periapical lesions. Mediators Inflamm. 2007;2007:38210.
30. Khayat B, Jouanny G. Microsurgical Endodontics. Quintessence Publishing (IL); 2019.
31. Ma J, Al-Ashaw AJ, Shen Y, Gao Y, Yang Y, Zhang C, et al. Efficacy of ProTaper Universal Rotary Retreatment system for gutta-percha removal from oval root canals: a micro-computed tomography study. J Endod. 2012 Nov;38(11):1516–20.
32. Indi S, Desai SR, Hambire A, Mustafa M, Almokhatieb AA, Abuelqomsan MAS, et al. Comparison of the Time Required by Six Different Retreatment Techniques for Retrieval of Gutta-Percha: An In Vitro Study [Internet]. European Journal of General Dentistry. 2022. Available from: http://dx.doi.org/10.1055/s-0042-1750089
33. Siddique R, Nivedhitha MS, Ranjan M, Jacob B, Solete P. Comparison of antibacterial effectiveness of three rotary file system with different geometry in infected root canals before and after instrumentation-a double-blinded randomized controlled clinical trial. BDJ Open. 2020 Jun 8;6:8.
34. Siddique R, Nivedhitha MS. Effectiveness of rotary and reciprocating systems on microbial reduction: A systematic review. J Conserv Dent. 2019 Mar-Apr;22(2):114–22.
35. Letterio JJ, Roberts AB. Transforming growth factor-β1-deficient mice: identification of isoform- specific activities in vivo [Internet]. Vol. 59, Journal of Leukocyte Biology. 1996. p. 769–74. Available from: http://dx.doi.org/10.1002/jlb.59.6.769
36. Khayat B, Jouanny G. Microsurgical Endodontics. Quintessence Publishing (IL); 2019.
37. Pratheebha C, Gayathri R, Veeraraghavan VP, Kavitha S. Knowledge, awareness, and perception on root canal treatment among South Indian population - A survey. J Adv Pharm Technol Res. 2022 Nov;13(Suppl 1):S302–7.
38. Joyce ME, Roberts AB, Sporn MB, Bolander ME. Transforming growth factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur [Internet]. Vol. 110, Journal of Cell Biology. 1990. p. 2195–207. Available from: http://dx.doi.org/10.1083/jcb.110.6.2195
39. Waris V, Xu JW, Nordsletten L, Sorsa T, Santavirta S, Konttinen YT. Basic Fibroblast Growth Factor (bFGF) in the Synovial-like Membrane around Loose Total Hip Prostheses [Internet]. Vol. 25, Scandinavian Journal of Rheumatology. 1996. p. 257–62. Available from: http://dx.doi.org/10.3109/03009749609069995
40. Crane JL, Zhao L, Frye JS, Xian L, Qiu T, Cao X. IGF-1 Signaling is Essential for Differentiation of Mesenchymal Stem Cells for Peak Bone Mass [Internet]. Vol. 1, Bone Research. 2013. p. 186–
94. Available from: http://dx.doi.org/10.4248/br201302007
41. Fujita T, Azuma Y, Fukuyama R, Hattori Y, Yoshida C, Koida M, et al. Runx2 induces osteoblast and chondrocyte differentiation and enhances their migration by coupling with PI3K-Akt signaling [Internet]. Vol. 166, Journal of Cell Biology. 2004. p. 85–95. Available from: http://dx.doi.org/10.1083/jcb.200401138
42. Xi G, Shen X, Rosen CJ, Clemmons DR. IRS-1 Functions as a Molecular Scaffold to Coordinate IGF-I/IGFBP-2 Signaling During Osteoblast Differentiation [Internet]. Vol. 31, Journal of Bone
and Mineral Research. 2016. p. 1300–14. Available from: http://dx.doi.org/10.1002/jbmr.2791
43. Nasim I, Professor and Head, Department of Conservative Dentistry and Endodontics, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai - 600077, India. Effect of nanoparticles based root canal disinfectants on Enterococcus faecalis - A systematic review. Int J Dent Oral Sci. 2021 Jun 30;2898–904.
44. Roman-Blas JA, Stokes DG, Jimenez SA. Modulation of TGF-β signaling by proinflammatory cytokines in articular chondrocytes [Internet]. Vol. 15, Osteoarthritis and Cartilage. 2007. p. 1367–77. Available from: http://dx.doi.org/10.1016/j.joca.2007.04.011
45. Evaluation of an Ultrasonic Technique to Remove Fractured Rotary Nickel-Titanium Endodontic Instruments from Root Canals: Clinical Cases. J Endod. 2003 Nov 1;29(11):764–7.
46. Natanasabapathy V, Durvasulu A, Krithikadatta J, Namasivayam A, Deivanayagam K, Manali S, et al. Current Trends in the Use of Irrigant Activation Techniques Among Endodontists & Post-Graduate Dental Students in India -A Knowledge, Attitude and Practice Based Survey. Eur Endod J. 2020 May 22;5(2):73–80.
47. Mitchell K, Shah JP, Tsytsikova LV, Campbell AM, Affram K, Symes AJ. LPS antagonism of TGF-β signaling results in prolonged survival and activation of rat primary microglia. J Neurochem. 2014 Apr;129(1):155–68.
48. Kulkarni S, Wahane K, Daokar S, Patil K, Patel K, Thorat T. An assessment of the efficacy of a rotary and a reciprocating retreatment file system for removal of gutta-percha from root canals: An in vitro cone-beam computed tomography study. Endodontology. 2021;33(1):20.
49. Azim AA, Wang HH, Tarrosh M, Azim KA, Piasecki L. Comparison between Single-file Rotary Systems: Part 1—Efficiency, Effectiveness, and Adverse Effects in Endodontic Retreatment [Internet]. Vol. 44, Journal of Endodontics. 2018. p. 1720–4. Available from: http://dx.doi.org/10.1016/j.joen.2018.07.022
50. Rodrigues CT, Duarte MAH, de Almeida MM, de Andrade FB, Bernardineli N. Efficacy of CM- Wire, M-Wire, and Nickel-Titanium Instruments for Removing Filling Material from Curved Root Canals: A Micro–Computed Tomography Study [Internet]. Vol. 42, Journal of Endodontics. 2016. p. 1651–5. Available from: http://dx.doi.org/10.1016/j.joen.2016.08.012
51. Divya S, Jeevanandan G, Sujatha S, Subramanian EMG, Ravindran V. Comparison of quality of obturation and post-operative pain using manual vs rotary files in primary teeth - A randomised clinical trial. Indian J Dent Res. 2019 Nov-Dec;30(6):904–8.
52. Briard N, Dadoun F, Pommier G, Sauze N, Lebouc Y, Oliver C, et al. IGF-I/IGFBPs system response to endotoxin challenge in sheep. J Endocrinol. 2000 Mar;164(3):361–9.
53. Endodontic retreatment—Case selection and technique. Part 2: Treatment planning for retreatment. J Endod. 1988 Dec 1;14(12):607–14.
54. A statistical analysis of surgical and nonsurgical endodontic retreatment cases. J Endod. 1989 Jun 1;15(6):261–6.
55. Results of endodontic retreatment: A randomized clinical study comparing surgical and nonsurgical procedures. J Endod. 1999 Dec 1;25(12):814–7.
56. Panchal V, Gurunathan D, Muralidharan NP. Comparison of antibacterial efficacy of cinnamon extract, neem extract as irrigant and sodium hypochlorite against : An study. Indian J Dent Res. 2020 Jan-Feb;31(1):124–8.
57. Fan J, Molina PE, Gelato MC, Lang CH. Differential tissue regulation of insulin-like growth factor-I content and binding proteins after endotoxin [Internet]. Vol. 134, Endocrinology. 1994. p. 1685–92. Available from: http://dx.doi.org/10.1210/endo.134.4.7511091
58. Soto L, Martin AI, Millan S, Vara E, Lopez-Calderon A. Effects of endotoxin lipopolysaccharide administration on the somatotropic axis [Internet]. Vol. 159, Journal of Endocrinology. 1998. p. 239–46. Available from: http://dx.doi.org/10.1677/joe.0.1590239
59. Chang CH, Wen ZH, Wang SK, Duh CY. New anti-inflammatory steroids from the Formosan soft coral Clavularia viridis [Internet]. Vol. 73, Steroids. 2008. p. 562–7. Available from: http://dx.doi.org/10.1016/j.steroids.2008.01.007
60. Fernando IPS, Shanura Fernando IP, Asanka Sanjeewa KK, Kim HS, Kim SY, Lee SH, et al. Identification of sterols from the soft coral Dendronephthya gigantea and their anti-inflammatory potential [Internet]. Vol. 55, Environmental Toxicology and Pharmacology. 2017. p. 37–43. Available from: http://dx.doi.org/10.1016/j.etap.2017.08.003
61. Kobori M. Anti-inflammatory, Anti-cancer and Anti-diabetic Properties of Sterols and Polyphenols in Vegetables and Fruits [Internet]. Vol. 56, Nippon Shokuhin Kagaku Kogaku Kaishi. 2009. p. 614–9. Available from: http://dx.doi.org/10.3136/nskkk.56.614
62. Bhagavaldas MC, Diwan A, Kusumvalli S, Pasha S, Devale M, Chava DC. Efficacy of two rotary retreatment systems in removing Gutta-percha and sealer during endodontic retreatment with or without solvent: A comparative study. J Conserv Dent. 2017 Jan-Feb;20(1):12–6.
63. Sundar S, Varghese A, Datta KJ, Natanasabapathy V. Effect of guided conservative endodontic access and different file kinematics on debris extrusion in mesial root of the mandibular molars: An study. J Conserv Dent. 2022 Sep 12;25(5):547–54.
64. Hu J, Yang B, Lin X, Zhou X, Yang X, Long L, et al. Chemical and Biological Studies of Soft Corals of the Nephtheidae Family [Internet]. Vol. 8, Chemistry & Biodiversity. 2011. p. 1011–
32. Available from: http://dx.doi.org/10.1002/cbdv.201000105
65. Huang YC, Wen ZH, Wang SK, Hsu CH, Duh CY. New anti-inflammatory 4-methylated steroids from the Formosan soft coral Nephthea chabroli [Internet]. Vol. 73, Steroids. 2008. p. 1181–6. Available from: http://dx.doi.org/10.1016/j.steroids.2008.05.007
66. Khayat B, Jouanny G. Microsurgical Endodontics. Quintessence Publishing (IL); 2019.
67. Ma J, Al-Ashaw AJ, Shen Y, Gao Y, Yang Y, Zhang C, et al. Efficacy of ProTaper Universal Rotary Retreatment system for gutta-percha removal from oval root canals: a micro-computed tomography study. J Endod. 2012 Nov;38(11):1516–20.
68. Indi S, Desai SR, Hambire A, Mustafa M, Almokhatieb AA, Abuelqomsan MAS, et al. Comparison of the Time Required by Six Different Retreatment Techniques for Retrieval of Gutta-Percha: An In Vitro Study [Internet]. European Journal of General Dentistry. 2022. Available from: http://dx.doi.org/10.1055/s-0042-1750089
69. Wong R. Conventional endodontic failure and retreatment. Dent Clin North Am. 2004 Jan;48(1):265–89.
70. Nivedhitha N, Professor and Head, Department of Conservative Dentistry and Endodontics, Saveetha Dental college and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India. Comparing the effectiveness of various irrigant activation techniques with conventional needle irrigation - A systematic review. Int J Dent Oral Sci. 2021 May 30;2626–31.
71. Siddique R, Nivedhitha MS, Jacob B. Quantitative analysis for detection of toxic elements in various irrigants, their combination (precipitate), and para-chloroaniline: An inductively coupled plasma mass spectrometry study. J Conserv Dent. 2019 Jul-Aug;22(4):344–50.
72. Paz SM de la, la Paz SM de, Fernández-Arche Á, Ángel-Martín M, García-Giménez MD. The sterols isolated from Evening Primrose oil modulate the release of proinflammatory mediators [Internet]. Vol. 19, Phytomedicine. 2012. p. 1072–6. Available from: http://dx.doi.org/10.1016/j.phymed.2012.06.008
73. Identification of Sitosterol as in Vitro Anti-Inflammatory Constituent in Moringa oleifera [Internet]. Available from: http://dx.doi.org/10.1021/acs.jafc.8b04555.s001
74. Sagare SV, Chandra P, Kaur T, Ganorkar O, Khade A, Mehta SD. A comparative study of the efficacy of WaveOne and NeoEndo retreatment file system for the removal of Gutta percha from the root canal. J Pharm Bioallied Sci. 2021 Nov;13(Suppl 2):S1682–5.
75. Hima Sandeep A, Senior Lecturer, Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences. Prevalence and associated factors of root canal treated mandibular anterior teeth with and without post endodontic crowns. Int J Dent Oral Sci. 2019 Nov 30;87–91.
76. Choi JN, Choi YH, Lee JM, Noh IC, Park JW, Choi WS, et al. Anti-inflammatory effects of β-
sitosterol-β-D-glucoside from Trachelospermum jasminoides (Apocynaceae) in lipopolysaccharide-stimulated RAW 264.7 murine macrophages. Nat Prod Res. 2012 Jan 31;26(24):2340–3.
77. Kassen A, Berges R, Senge T. Effect of Beta–Sitosterol on Transforming Growth Factor–Beta– 1 Expression and Translocation Protein Kinase C Alpha in Human Prostate Stromal Cells in vitro [Internet]. Vol. 37, European Urology. 2000. p. 735–41. Available from: http://dx.doi.org/10.1159/000020227
78. Kulkarni S, Wahane K, Daokar S, Patil K, Patel K, Thorat T. An assessment of the efficacy of a rotary and a reciprocating retreatment file system for removal of gutta-percha from root canals: An in vitro cone-beam computed tomography study. Endodontology. 2021;33(1):20.
79. Azim AA, Wang HH, Tarrosh M, Azim KA, Piasecki L. Comparison between Single-file Rotary Systems: Part 1—Efficiency, Effectiveness, and Adverse Effects in Endodontic Retreatment [Internet]. Vol. 44, Journal of Endodontics. 2018. p. 1720–4. Available from: http://dx.doi.org/10.1016/j.joen.2018.07.022
80. Sagare SV, Chandra P, Kaur T, Ganorkar O, Khade A, Mehta SD. A comparative study of the efficacy of WaveOne and NeoEndo retreatment file system for the removal of Gutta percha from the root canal. J Pharm Bioallied Sci. 2021 Nov;13(Suppl 2):S1682–5.
81. Janani K, Teja KV, Ajitha P, Sandhya R. Evaluation of tissue inflammatory response of four intracanal medicament - An animal study. J Conserv Dent. 2020 Dec 4;23(3):216–20.
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Jul 17, 2023
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Nishitha Arun, Sindhu Ramesh, Selvaraj Jayaraman, & Anjali Sankar. (2023). EFFECT OF BETA- SITOSTEROL ON EXPRESSION OF TGF- β, IGF-1 AND TNF-α mRNA IN LPS INDUCED FIBROBLAST CELL LINE- A MOLECULAR STUDY. Journal of Population Therapeutics and Clinical Pharmacology, 30(6), 511-521. https://doi.org/10.47750/jptcp.2023.30.06.059
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Author Biographies
Nishitha Arun
Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India
Sindhu Ramesh
Professor, Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India
Selvaraj Jayaraman
Associate Professor, Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India
Anjali Sankar
Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India
How to Cite
Nishitha Arun, Sindhu Ramesh, Selvaraj Jayaraman, & Anjali Sankar. (2023). EFFECT OF BETA- SITOSTEROL ON EXPRESSION OF TGF- β, IGF-1 AND TNF-α mRNA IN LPS INDUCED FIBROBLAST CELL LINE- A MOLECULAR STUDY. Journal of Population Therapeutics and Clinical Pharmacology, 30(6), 511-521. https://doi.org/10.47750/jptcp.2023.30.06.059