Bioactivity of Brevibacillus laterosporus againt fungal and bacterial growth
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Keywords
MIC, MBC, Bioactivity, Brevibacillus laterosporus, antibiotic
Abstract
The acquisition and spread of antibiotic resistance among pathogenic bacteria pose a major threat to public health. Thus, there is a need for new antimicrobials that can be used as bacteriostatic alternatives to conventional antibiotics. Antibacterial compounds produced by genus Brevibacillus have been studied in recent years. They are an important tool for biological control. Different species of Brevibacillus show broad-spectrum antimicrobial activity, including activity against bacteria and fungi. A variety of molecules, including proteins and antibiotics, have been associated with their observed pathogenicity and mode of action. The antifungal and antibacterial properties of some Brevibacillus species found medical interest, linked to the production of antibiotics with therapeutic effects. The aim of this study is to isolate and diagnose Brevibacillus spp. from soil and show its toxic activity against bacteria and fungi by studying the protein extract of the bacteria. Soil samples were collected, Brevibacillus laterosporus was isolated, the protein mass was extracted from these bacteria, and its bioactivity was tested on the growth of the selected bacterial strains and yeast strains. We found that the protein extracted has in vitro bioactivity that inhibits the growth of bacteria and fungi under the current study. Minimum Inhibitory and Bactericidal Concentration (MIC&MBC) of extracted protein was identified against Staphylococcus aureus, Serratia spp., Candida albicans, and Candida tropicalis. We found that we can prepare a protein extract with MIC and MBC values from Brevibacillus laterosporus as an antibiotic, further study is required to confirm these results.
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