Stabilization Of Silver Nanoparticles Using Whey Proteins
Main Article Content
Keywords
Silver nanoparticles, whey protein, cell culture, anti-inflammatory
Abstract
Introduction: Silver nanoparticles (AgNPs) are widely recommended as a substitute for antibiotics because the antibiotics are becoming ineffective and the dosages are increasing day by day.
Materials and methods: A nanoparticle formulation was prepared by adding 100 ml of water and 0.32 g of silver nitrate and then heating the mixture. Then, 10 ml of whey protein was slowly drizzled into this concoction and obtained a red hue.
Results: The particle formation was seen using UV spectroscopy. SEM analysis visualised the formation of the nanoparticles and they were formed to be a uniform size. MTT assay confirmed the viability of the cells. The antibacterial and anti-inflammatory properties of AgNPs were confirmed.
Conclusion: Silver nanoparticles synthesized from whey protein are stable and consistent in size.
References
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2. S.T. Abitha, S. Rajeshkumar, T. Lakshmi, A. Roy, Cytotoxic effects of silver nanoparticles synthesized using amla fruit seed, Drug Invention Today 11(9) (2019).
3. F.K. Alsammarraie, W. Wang, P. Zhou, A. Mustapha, M. Lin, Green synthesis of silver nanoparticles using turmeric extracts and investigation of their antibacterial activities, Colloids and Surfaces B: Biointerfaces 171 (2018) 398-405.
4. M. Kim, S.-C. Jee, S.K. Shinde, B.M. Mistry, R.G. Saratale, G.D. Saratale, G.S. Ghodake, D.-Y. Kim, J.-S. Sung, A.A. Kadam, Green-synthesis of anisotropic peptone-silver nanoparticles and its potential application as anti-bacterial agent, Polymers 11(2) (2019) 271.
5. A. Rodríguez-Calderón, M. Contreras-García, R. Nuñez-Anita, Synthesis of silver nanoparticles coated with bovine serum albumin for application as vaccine carriers, Journal of Physics: Conference Series, IOP Publishing, 2022, p. 012014.
6. S. Agnihotri, K. Bhagwani, C. Sunny, S. Panicker, Antibacterial activity of silver nanoparticles synthesized from buttermilk, Drug Invention Today 11(10) (2019).
7. O. Pawar, N. Deshpande, S. Dagade, S. Waghmode, P. Nigam Joshi, Green synthesis of silver nanoparticles from purple acid phosphatase apoenzyme isolated from a new source Limonia acidissima, Journal of Experimental Nanoscience 11(1) (2016) 28-37.
8. L. Donato, E. Kolodziejcyk, M. Rouvet, Mixtures of whey protein microgels and soluble aggregates as building blocks to control rheology and structure of acid induced cold-set gels, Food Hydrocolloids 25(4) (2011) 734-742.
9. S. Srisod, K. Motina, T. Inprasit, P. Pisitsak, A green and facile approach to durable antimicrobial coating of cotton with silver nanoparticles, whey protein, and natural tannin, Progress in Organic Coatings 120 (2018) 123-131.
10. Felicita, A. Sumathi, Shyamala Chandrasekar, and K. K. Shanthasundari. 2012. “Determination of Craniofacial Relation among the Subethnic Indian Population: A Modified Approach - (Sagittal Relation).” Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research 23 (3): 305–12.
11. Lakshmi, T., Vidya Krishnan, R. Rajendran, and N. Madhusudhanan. 2015. “Azadirachta Indica: A Herbal Panacea in Dentistry - An Update.” Pharmacognosy Reviews 9 (17): 41–44.
12. Menon, Aniruddh, and M. S. Thenmozhi. 2016. “Correlation between Thyroid Function and Obesity.” Journal of Advanced Pharmaceutical Technology & Research 9 (10): 1568.
13. Rajeshkumar, S., Soumya Menon, S. Venkat Kumar, Murtaza M. Tambuwala, Hamid A. Bakshi, Meenu Mehta, Saurabh Satija, et al. 2019. “Antibacterial and Antioxidant Potential of Biosynthesized Copper Nanoparticles Mediated through Cissus Arnotiana Plant Extract.” Journal of Photochemistry and Photobiology. B, Biology 197 (August): 111531.
14. Sahu, Devashri, G. M. Kannan, and R. Vijayaraghavan. 2014. “Size-Dependent Effect of Zinc Oxide on Toxicity and Inflammatory Potential of Human Monocytes.” Journal of Toxicology and Environmental Health. Part A 77 (4): 177–91.
15. Saravanan, A., P. Senthil Kumar, S. Jeevanantham, S. Karishma, B. Tajsabreen, P. R. Yaashikaa, and B. Reshma. 2021. “Effective Water/wastewater Treatment Methodologies for Toxic Pollutants Removal: Processes and Applications towards Sustainable Development.” Chemosphere 280 (October): 130595.
16. Sathivel, Arumugam, Hanumantha Rao Balaji Raghavendran, Periasamy Srinivasan, and Thiruvengadam Devaki. 2008. “Anti-Peroxidative and Anti-Hyperlipidemic Nature of Ulva Lactuca Crude Polysaccharide on D-Galactosamine Induced Hepatitis in Rats.” Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research Association 46 (10): 3262–67.
17. Sekar, Durairaj, Ganesh Lakshmanan, Panagal Mani, and M. Biruntha. 2019. “Methylation-Dependent Circulating microRNA 510 in Preeclampsia Patients.” Hypertension Research: Official Journal of the Japanese Society of Hypertension 42 (10): 1647–48.
18. Thejeswar, E. P., and M. S. Thenmozhi. 2015. “Educational Research-iPad System vs Textbook System.” Journal of Advanced Pharmaceutical Technology & Research 8 (8): 1158.
19. Wang, Yu, Yayuan Zhang, Yan Guo, Jianguang Lu, Vishnu Priya Veeraraghavan, Surapaneni Krishna Mohan, Chao Wang, and Xiaoguang Yu. 2019. “Synthesis of Zinc Oxide Nanoparticles from Marsdenia Tenacissima Inhibits the Cell Proliferation and Induces Apoptosis in Laryngeal Cancer Cells (Hep-2).” Journal of Photochemistry and Photobiology. B, Biology 201 (December): 111624.
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Published
Jun 24, 2023
Article Details
How to Cite
Aashruthaa S, Suganya Panneer Selvam, Saranya Kannan, Ramya Ramadoss, Sandhya Sundar, & Pratibha Ramani. (2023). Stabilization Of Silver Nanoparticles Using Whey Proteins. Journal of Population Therapeutics and Clinical Pharmacology, 30(16), 266-270. https://doi.org/10.47750/jptcp.2023.30.16.035
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Aashruthaa S, Suganya Panneer Selvam, Saranya Kannan, Ramya Ramadoss, Sandhya Sundar, & Pratibha Ramani. (2023). Stabilization Of Silver Nanoparticles Using Whey Proteins. Journal of Population Therapeutics and Clinical Pharmacology, 30(16), 266-270. https://doi.org/10.47750/jptcp.2023.30.16.035