Nano spatial elemental characterization of deep pits in molar teeth
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Keywords
molar, Pathology, chemical, microscope
Abstract
Background: The caries of the pits and fissure follow the direction of the enamel rod, characteristically forming a triangular or conical lesion, the apex of which is on the outer surface and its base towards the DEJ. The caries of the pits and fissure follow the direction of the enamel rod, characteristically forming a triangular or conical lesion, the apex of which is on the outer surface and its base towards the DEJ. Pits and fissures cause more cavitation than cavitation on smooth surfaces.
Aim: Nano spatial assessment of deep pits in molars and premolars.
Objective: Characterization of pit and fissure for better treatment planning of treatment of pit and fissure sealants.
Materials and Methods: Field Emission Scanning electron microscope (FE-SEM – Jeol JSM – IT800, Tokyo, Japan) was used to visualize the tooth sections on the glass cover slip at a magnification of 5 μm. Sections will be dehydrated with 70% ethyl alcohol for 10 sec and nitrogen gas was applied for drying. After critical point drying, the sections were sputter-coated with platinum to induce conductivity. Finally, images were captured and projected. Further energy dispersive x-ray spectroscopy was used for elemental analyses and chemical characterization.
Conclusion: Base of the pit revealed more mineralization than the upper and middle third. Interestingly the zones adjacent to the pits were more mineralized
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