Assessment of the anti-inflammatory effect of a novel Chitosan-Sunflower seed gel – an in vitro study
Main Article Content
Keywords
anti-inflammatory activity, green synthesis, chitosan, sunflower seeds
Abstract
Objective: This study aimed to evaluate the anti inflammatory activity of a novel chitosan- sunflower seed gel.
Materials and Methods: A novel chitosan-sunflower seed gel was prepared. The antiinflammatory activity of the prepared gel, in five different concentrations (10, 20. 30, 40 and 50μl), was tested in a dual wavelength spectrophotometer.
Result: The 40μl dilution of the gel had the best antiinflammatory activity, followed by 50μl, 30μl, 20μl and 10μl dilutions in decreasing order.
Conclusion: The chitosan-sunflower seed gel is a new and potential alternative as a wound healing agent with excellent anti-inflammatory activity that requires further clinical assessment to better establish appropriate protocols for treatment.
References
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14. Kapadia, G.J. et al. (2002) ‘Chemopreventive effect of resveratrol, sesamol, sesame oil and sunflower oil in the Epstein-Barr virus early antigen activation assay and the mouse skin two-stage carcinogenesis’, Pharmacological research: the official journal of the Italian Pharmacological Society, 45(6), pp. 499–505.
15. Kuo, Y.-S. et al. (2022) ‘Evaluation of the Preventive Effects of Fish Oil and Sunflower Seed Oil on the Pathophysiology of Sepsis in Endotoxemic Rats’, Frontiers in nutrition, 9, p. 857255.
16. Leuba, J.L. and Stossel, P. (1986) ‘Chitosan and Other Polyamines: Antifungal Activity and Interaction with Biological Membranes’, Chitin in Nature and Technology, pp. 215–222. Available at: https://doi.org/10.1007/978-1-4613-2167-5_29.
17. Lopez-Lopez, J. et al. (2015) ‘Efficacy of chlorhexidine, dexpanthenol, allantoin and chitosan gel in comparison with bicarbonate oral rinse in controlling post-interventional inflammation, pain and cicatrization in subjects undergoing dental surgery’, Current medical research and opinion, 31(12), pp. 2179–2183.
18. Manhezi, A.C., Bachion, M.M. and Pereira, A.L. (2008) ‘[The use of essential fatty acids in the treatments of wounds]’, Revista brasileira de enfermagem, 61(5), pp. 620–628.
19. Mathew, M.G. et al. (2020) ‘Evaluation of adhesion of Streptococcus mutans, plaque accumulation on zirconia and stainless steel crowns, and surrounding gingival inflammation in primary molars: randomized controlled trial’, Clinical oral investigations, 24(9), pp. 3275–3280.
20. Norlén, L. (2016) ‘Is Oil a Balsam for Baby Skin?’, Acta dermato-venereologica, p. 291.
21. Ramadurai, N. et al. (2019) ‘Effectiveness of 2% Articaine as an anesthetic agent in children: randomized controlled trial’, Clinical oral investigations, 23(9), pp. 3543–3550.
22. Ramesh, A. et al. (2018) ‘Comparative estimation of sulfiredoxin levels between chronic periodontitis and healthy patients - A case-control study’, Journal of periodontology, 89(10), pp. 1241–1248.
23. Siddique, R. et al. (2019) ‘Qualitative and quantitative analysis of precipitate formation following interaction of chlorhexidine with sodium hypochlorite, neem, and tulsi’, Journal ofconservative dentistry: JCD, 22(1), pp. 40–47.
24. Sridharan, G. et al. (2019) ‘Evaluation of salivary metabolomics in oral leukoplakia and oral squamous cell carcinoma’, Journal of oral pathology & medicine: official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 48(4), pp. 299–306.
25. Teja, K.V., Ramesh, S. and Priya, V. (2018) ‘Regulation of matrix metalloproteinase-3 gene expression in inflammation: A molecular study’, Journal of conservative dentistry: JCD, 21(6), pp. 592–596.
26. Torres-Rosas, R. et al. (2020) ‘Anti-
Inflammatory and Antibacterial Activity of the Chitosan/Chlorhexidine Gel Commercial Preparation for Postexodontia Treatment: An In Vitro Study’, European journal of dentistry, 14(3), pp. 397–403.
27. Vargas Villanueva, J.G. et al. (2019) ‘Bio-adhesion evaluation of a chitosan-based bone bio-adhesive’, International Journal of Adhesion and Adhesives, 92, pp. 80–88.
28. Vijayashree Priyadharsini, J. (2019) ‘In silico validation of the non-antibiotic drugs acetaminophen and ibuprofen as antibacterial agents against red complex pathogens’, Journal of periodontology, 90(12), pp. 1441–1448.
2. Cooke, A. et al. (2016) ‘Olive Oil, Sunflower Oil or no Oil for Baby Dry Skin or Massage: A Pilot, Assessor-blinded, Randomized Controlled Trial (the Oil in Baby SkincaRE [OBSeRvE] Study)’, Acta dermato-venereologica, 96(3), pp. 323–330.
3. Danby, S.G. et al. (2013) ‘Effect of olive and sunflower seed oil on the adult skin barrier: implications for neonatal skin care’, Pediatric dermatology, 30(1), pp. 42–50.
4. Das, U., Behera, S.S. and Pramanik, K. (2017) ‘Ethno-Herbal-Medico in Wound Repair: An Incisive Review’, Phytotherapy research: PTR, 31(4), pp. 579–590.
5. Daud, F.V. et al. (2011) ‘The use of ozonized oil in the treatment of dermatophitosis caused by Microsporum canis in rabbits’, Brazilian Journal of Microbiology, pp. 274–281. Available at: https://doi.org/10.1590/s1517-83822011000100035.
6. Duraisamy, R. et al. (2019) ‘Compatibility of Nonoriginal Abutments With Implants: Evaluation of Microgap at the Implant-Abutment Interface, With Original and Nonoriginal Abutments’, Implant dentistry, 28(3), pp. 289–295.
7. Ezhilarasan, D., Apoorva, V.S. and Ashok Vardhan, N. (2019) ‘Syzygium cumini extract induced reactive oxygen species-mediated apoptosis in human oral squamous carcinoma cells’, Journal of oral pathology & medicine: official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 48(2), pp. 115–121.
8. Ferreira, M. do R.A.B. et al. (2021) ‘Violência Doméstica Contra A Mulher No Contexto Da Atenção Primária À Saúde: Revisão Integrativa/ Domestic Violence Against Women In The Context Of Primary Health Care: Integrative Review’, Brazilian Journal of Development, pp. 6286–6306. Available at: https://doi.org/10.34117/bjdv7n1-427.
9. Fong, D. and Hoemann, C.D. (2018) ‘Chitosan immunomodulatory properties: perspectives on the impact of structural properties and dosage’, Future science OA, 4(1), p. FSO225.
10. Frisone, F. (no date) ‘Codici antichi, modelli moderni : l’epigrafia e l’interpretazione dei rituali funerari nelle società antiche’, L’écriture et l’espace de la mort. Épigraphie et nécropoles à l’époque préromaine [Preprint]. Available at: https://doi.org/10.4000/books.efr.2724.
11. Guo, S. and Dipietro, L.A. (2010) ‘Factors affecting wound healing’, Journal of dental research, 89(3), pp. 219–229.
12. J, P.C. et al. (2018) ‘Prevalence and measurement
of anterior loop of the mandibular canal using CBCT: A cross sectional study’, Clinical implant dentistry and related research, 20(4), pp. 531–534.
13. Kamjumphol, W., Chareonsudjai, P. and Chareonsudjai, S. (2018) ‘Antibacterial activity of chitosan against Burkholderia pseudomallei’, MicrobiologyOpen, 7(1). Available at: https://doi.org/10.1002/mbo3.534.
14. Kapadia, G.J. et al. (2002) ‘Chemopreventive effect of resveratrol, sesamol, sesame oil and sunflower oil in the Epstein-Barr virus early antigen activation assay and the mouse skin two-stage carcinogenesis’, Pharmacological research: the official journal of the Italian Pharmacological Society, 45(6), pp. 499–505.
15. Kuo, Y.-S. et al. (2022) ‘Evaluation of the Preventive Effects of Fish Oil and Sunflower Seed Oil on the Pathophysiology of Sepsis in Endotoxemic Rats’, Frontiers in nutrition, 9, p. 857255.
16. Leuba, J.L. and Stossel, P. (1986) ‘Chitosan and Other Polyamines: Antifungal Activity and Interaction with Biological Membranes’, Chitin in Nature and Technology, pp. 215–222. Available at: https://doi.org/10.1007/978-1-4613-2167-5_29.
17. Lopez-Lopez, J. et al. (2015) ‘Efficacy of chlorhexidine, dexpanthenol, allantoin and chitosan gel in comparison with bicarbonate oral rinse in controlling post-interventional inflammation, pain and cicatrization in subjects undergoing dental surgery’, Current medical research and opinion, 31(12), pp. 2179–2183.
18. Manhezi, A.C., Bachion, M.M. and Pereira, A.L. (2008) ‘[The use of essential fatty acids in the treatments of wounds]’, Revista brasileira de enfermagem, 61(5), pp. 620–628.
19. Mathew, M.G. et al. (2020) ‘Evaluation of adhesion of Streptococcus mutans, plaque accumulation on zirconia and stainless steel crowns, and surrounding gingival inflammation in primary molars: randomized controlled trial’, Clinical oral investigations, 24(9), pp. 3275–3280.
20. Norlén, L. (2016) ‘Is Oil a Balsam for Baby Skin?’, Acta dermato-venereologica, p. 291.
21. Ramadurai, N. et al. (2019) ‘Effectiveness of 2% Articaine as an anesthetic agent in children: randomized controlled trial’, Clinical oral investigations, 23(9), pp. 3543–3550.
22. Ramesh, A. et al. (2018) ‘Comparative estimation of sulfiredoxin levels between chronic periodontitis and healthy patients - A case-control study’, Journal of periodontology, 89(10), pp. 1241–1248.
23. Siddique, R. et al. (2019) ‘Qualitative and quantitative analysis of precipitate formation following interaction of chlorhexidine with sodium hypochlorite, neem, and tulsi’, Journal ofconservative dentistry: JCD, 22(1), pp. 40–47.
24. Sridharan, G. et al. (2019) ‘Evaluation of salivary metabolomics in oral leukoplakia and oral squamous cell carcinoma’, Journal of oral pathology & medicine: official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 48(4), pp. 299–306.
25. Teja, K.V., Ramesh, S. and Priya, V. (2018) ‘Regulation of matrix metalloproteinase-3 gene expression in inflammation: A molecular study’, Journal of conservative dentistry: JCD, 21(6), pp. 592–596.
26. Torres-Rosas, R. et al. (2020) ‘Anti-
Inflammatory and Antibacterial Activity of the Chitosan/Chlorhexidine Gel Commercial Preparation for Postexodontia Treatment: An In Vitro Study’, European journal of dentistry, 14(3), pp. 397–403.
27. Vargas Villanueva, J.G. et al. (2019) ‘Bio-adhesion evaluation of a chitosan-based bone bio-adhesive’, International Journal of Adhesion and Adhesives, 92, pp. 80–88.
28. Vijayashree Priyadharsini, J. (2019) ‘In silico validation of the non-antibiotic drugs acetaminophen and ibuprofen as antibacterial agents against red complex pathogens’, Journal of periodontology, 90(12), pp. 1441–1448.
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Published
Jun 22, 2023
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How to Cite
Ojasvini Arora, Subhashree Rohinikumar, & S Rajeshkumar. (2023). Assessment of the anti-inflammatory effect of a novel Chitosan-Sunflower seed gel – an in vitro study. Journal of Population Therapeutics and Clinical Pharmacology, 30(15), 369-374. https://doi.org/10.47750/jptcp.2023.30.15.042
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All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Ojasvini Arora, Subhashree Rohinikumar, & S Rajeshkumar. (2023). Assessment of the anti-inflammatory effect of a novel Chitosan-Sunflower seed gel – an in vitro study. Journal of Population Therapeutics and Clinical Pharmacology, 30(15), 369-374. https://doi.org/10.47750/jptcp.2023.30.15.042