Real time and rapid detection of NDM 1 gene in beta lactam resistant bacteria using electrochemical assay based aptagene nanoreceptors
Main Article Content
Keywords
NDM-1 gene, aptamer binding, PCR amplification, aptasensor, single strand DNA
Abstract
Background: New Delhi Metallo b lactamase (NDM 1) is an enzyme that makes bacteria resistant to a broad range of beta lactam antibiotics
Methods: In this study, the target gene was NDM-1 of beta lactam resistant bacteria which isolated on Mueller Hinton Agar (MHA) and identified via DNA extraction and PCR amplification then compared with the electrochemical assay based aptamer nanoreceptors.
Results: The results were encouraged and indicate that specific DNA aptasensor identified signal based the aptamer NDM-1 gene binding and exhibited affinity for the target DNA single strand, resulting in the direct detection of the NDM-1 in complex clinical bacteria samples in compare with PCR amplification.
Conclusions: This novel electrochemical gene sensor method allows for the cost effective, rapid, specific, and more sensitive detection of the NDM-1.
References
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18. Khobragade S. Bacterial detection using an anharmonic acoustic aptasensor (Doctoral dissertation, Loughborough University); 2018 .
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20. Courbet A. Engineering next generation diagnostics through synthetic biology (Doctoral dissertation, Universite Montpellier); 2015.
21. Hendriksen R S, Vieira A R, Karlsmose S, Lo Fo Wong D M, Jensen A B, Wegener H C, Aarestrup F M. Global monitoring of Salmonella serovar distribution from the World Health Organization Global Foodborne Infections Network Country Data Bank: results of quality assured laboratories from 2001 to 2007. Foodborne pathogens and disease. 2011; 8(8): 887-900.
22. Courbet A. Engineering next generation diagnostics through synthetic biology (Doctoral dissertation, Universite Montpellier); 2015.
2. Heinz E, Ejaz H, Bartholdson Scott J, Wang N, Gujaran S, Pickard D, Strugnell, R A. Resistance mechanisms and population structure of highly drug resistant Klebsiella in Pakistan during the introduction of the carbapenemase NDM-1. Scientific reports. 2019; 9(1):1-13.
3. United, I A C G. No time to wait–securing the future from drug-resistant infections. Report to the Secretary General of the Nations. 2019.
4. D’Andrea M M, Fraziano M, Thaller M C, Rossolini G M. The urgent need for novel antimicrobial agents and strategies to fight antibiotic resistance. Antibiotics. 2019; 8(4): 254.
5. Frère J M. Beta-lactamases and bacterial resistance to antibiotics. Mol. Microbiol. 1995; 16: 385–395. [CrossRef] [PubMed]
6. Taubes G. The bacteria fight back. Science. 2008; 321: 356–361. [CrossRef] [PubMed].
7. Yong D, Toleman MA, Giske CG .Characterization of a new metallo-beta-lactamase gene, blaNdM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from india. antimicrob agents Chemother. 2009; 53: 5046-5054.
8. Groundwater P W, et al. New Delhi metallo-beta-lactamase-1: structure, inhibitors and detection of producers. Future Med. Chem. 2016; 8: 993–1012.
9. Khan A U, Maryam L, Zarrilli R. Structure, genetics and worldwide spread of New Delhi metallo-beta-lactamase (NDM): a threat to public health. BMC Microbiol. 2017;17: 101.
10. Yong D. et al. Characterization of a new metallo-beta-lactamase gene, bla (NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob. Agents Chemother. 2009;53: 5046–5054.
11. Nordmann P, Poirel L, Carre¨r A, et al. How to detect NDM-1 producers. J Clin Microbiol 2011; 49: 718–21.
12. Shenoy KA, Jyothi EK, Ravikumar R. Phenotypic identification & molecular detection of bla (ndm-1) gene in multidrug resistant Gram-negative bacilli in a tertiary care centre. Indian J Med Res. 2014 Apr;139(4):625-31. PMID: 24927351; PMCID: PMC4078503.
13. Nabok A, Abu-Ali H, Takita S, Smith D P. Electrochemical detection of prostate cancer biomarker PCA3 using specific rna-based aptamer labelled with ferrocene. Chemosensors.2021; 9(4): 59.
14. Abu-Ali H, Ozkaya C, Davis F, Walch N, Nabok A. Electrochemical aptasensor for detection of opamine. Chemosensors. 20208;2: 28.
15. Abu-Ali H, Nabok A, Smith T J. Development of novel and highly specific ssDNA-aptamer-based electrochemical biosensor for rapid detection of mercury (II) and lead (II) ions in water. Chemosensors. 2019;7(2):27.
16. Lai Y H, Sun S C, Chuang M C. Biosensors with built-in biomolecular logic gates for practical applications. Biosensors. 2014;4(3):273-300.
17. Vsxcz S, Punjabi K, Ahamad N, Singh S, Bendale P, Banerjee R. Nanotechnology Approaches for Rapid Detection and Theranostics of Antimicrobial Resistant Bacterial Infections. ACS Biomaterials Science & Engineering.2022; 8:(6), 2232-2257.
18. Khobragade S. Bacterial detection using an anharmonic acoustic aptasensor (Doctoral dissertation, Loughborough University); 2018 .
19. Bruno, J G. Predicting the uncertain future of aptamer-based diagnostics and therapeutics. Molecules.2015;20(4): 6866-6887.
20. Courbet A. Engineering next generation diagnostics through synthetic biology (Doctoral dissertation, Universite Montpellier); 2015.
21. Hendriksen R S, Vieira A R, Karlsmose S, Lo Fo Wong D M, Jensen A B, Wegener H C, Aarestrup F M. Global monitoring of Salmonella serovar distribution from the World Health Organization Global Foodborne Infections Network Country Data Bank: results of quality assured laboratories from 2001 to 2007. Foodborne pathogens and disease. 2011; 8(8): 887-900.
22. Courbet A. Engineering next generation diagnostics through synthetic biology (Doctoral dissertation, Universite Montpellier); 2015.
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Published
Jun 4, 2023
Article Details
How to Cite
Hisham F. Mohammad, Hanan S. Makii, Abbas A. AL Hasani, & Muna M. AL Hammadani. (2023). Real time and rapid detection of NDM 1 gene in beta lactam resistant bacteria using electrochemical assay based aptagene nanoreceptors. Journal of Population Therapeutics and Clinical Pharmacology, 30(13), 354-361. https://doi.org/10.47750/jptcp.2023.30.13.036
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All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Hisham F. Mohammad, Hanan S. Makii, Abbas A. AL Hasani, & Muna M. AL Hammadani. (2023). Real time and rapid detection of NDM 1 gene in beta lactam resistant bacteria using electrochemical assay based aptagene nanoreceptors. Journal of Population Therapeutics and Clinical Pharmacology, 30(13), 354-361. https://doi.org/10.47750/jptcp.2023.30.13.036