Development of an RP-HPLC Method to estimation Glimepiride in Bulk and Solid Dosage Form
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Keywords
RP-HPLC Method, Estimation of Glimepiride, Bulk & Solid Dosage Form, Linearity, Accuracy, Precision, System suitability
Abstract
AIM- Two straightforward and touchy RP-HPLC techniques for the quantitative estimation of Glimepiride in mass and drug portion structures have been contrived in this review. MATERIAL & METHODS- Glimepiride was resolved utilizing a RP-HPLC technique using a C-18 evenness section and a portable period of methanol: phosphate support pH 4.0 (50:50 V/V). The versatile stage was siphoned at a pace of 0.5 ml/min, and the location was done at a frequency of 239 nm. RESULTS- 2.470 minutes was discovered to be the retention time. Linearity, Accuracy, Precision, System Suitability, LOD, LOQ, Ruggedness, and Robustness are all validated for this approach. The proposed method is an excellent way to get accurate results and has been demonstrated to be suitable for regular Glimepiride analysis in bulk and dose forms. CONCLUSION- The method was used to find out how much of a compound was included in commercial pharmaceutical dosage forms. The approach is straightforward, repeatable, and accurate, and it is a better option for routine quality control than other chromatographic techniques.
References
2. Mane VB,Babar S,Kulkarni N, “Development Of UV Spectrophotometric Method For The Simultaneous Estimation Of Simvastatine And Ezetimibe In Tablet Dosage Form By Simultaneous Equation AndAbsorbance Ratio Method” Int. J. of Pharma Res., 2011,3(3),1459-1466.
3. Dey S, De A,Mandal S,Pradhan P, “ Development And Validation Of Rp-Hplc Method For The Estimation Of Simvastatin In Bulk And Pharmaceutical Dosage Form” Indo. Ame. J. of Pharma Res., 2013,7376-7384.
4. Alsaad AAA, Alassadi EAS, Al-Salman HNK, Hussein HH. The Simultaneous Determination of Ibuprofen and Paracetamol in Pharmaceutical Formulations by High performance Liquid Chromatography with Ultraviolet Detection. Asian Journal of Pharmaceutics 2019; 13(2): 141-152.
5. Praveen Kumar S. N., Bhadre Gowda D. G., Vathsala Deepu C., Mantelingu K. and Rangappa K. S. “Simultaneous estimation of statins like pravastain, atorvastatin and simvastatin in bulk and pharmaceutical dosage form by means ofHigh-Performance Liquid Chromatography” J. of Chem and Pharma res. 2013, 5(5):359-364.
6. Nilesh Jain,Ruchi jain,Hemant swami, “RPHPLC Method for Simultaneous Estimation of Simvastatin and Ezetimibe in Bulk Drug and its CombinedDosage Form” Asian J. Research Chem. 1(1): July-Sept. 2008.
7. B.StephenRathinaraj, V.Rajamanickam, Ch.Rajveer, D.Kumaraswamy, Ganesh Shehraobangla3, A.Arunachalam, “ Int. j. of Pharma sci. and bio. ,2010,1 (4),325-330.
8. Al-Salman HNK. Analysis methods and qualitative diagnosis chromatographic for mixture of narcotic substances in seized materials. European Journal of Scientific Research 2017; 147(4): 403-411.
9. Rahman MU, Praveen G,Nayola NK, “Simultaneous Estimation Of Simvastatin And Ezetimibe In Pharmaceutical Tablet Dosage Forms By Rp-Hplc” Int. J. of Pharma, 2010,56-62
10. Jain N, Jain R, Swami H,Pandey S, “Spectrophotometric Method For Simultaneous Estimation Of Simvastatin And Ezetimibe In Bulk Drug And Its Combineddosage Form” Int. J. of Pharma and Pharma Sci.,2009, 1(1),170-175.
11. Singala V, Bhaskar R, “Simultaneous Estimation Of Simvastatin And Metformin Hydrochloride In Bulk And Solid Dosage Forms” Rasayan J.Chem, 2010,3(3),507-513.
12. Jyothi AP,Tejaswi K,Parimala SS, “Spectrophotometric Estimation of Simvastatin in Bulk and Tablet Dosage Form” Int. J. of Innovative Pharma Res., 2013,4(1),284-287.
13. Muzaffar I, Khalil NY, Alanazi AM, Al-Rasood KA. “A simple and sensitive high performance liquid chromatography assay with a fluorescence detector for determination of canagliflozin in human plasma” Anal. Methods, 2015, 7, 3028-3035.
14. Manasa S, Dhanlaxmi K, Reddy NG,Sreenivasa S. “Method Development and Validation of Dapagliflozin in API by RP-HPLC and UV-Spectroscopy” int. J. Of Pharma. Sci and Drug Res., 2014, 6(3),250-252.
15. Aubry AF , Gu H, Magnier R, Morgan L, Xu X, Tirmenstein M, Wang B, Deng Y, Cai J, Couerbe P,Arnold M., “Validated LCMS/MS methods for the determination of dapagliflozin, a sodiumglucose cotransporter 2 inhibitor in normal and ZDF rat plasma.” Bioanalysis. 2010 Dec;2(12):2001-2009.
16. AL-Salman HN, Ali ET, Almukhtar OA, Jabir MS. 2-benzhydrylsulfinyl-N-hydroxyacetamide Extracted from Fig: A good Therapeutic Agent against Staphylococcus Aureus. AIP Conference Proceedings 2020; 2213(1): 020223-5.
17. Jani BR, Shah KV and Kapupara PP. “Development and Validation of UV Spectroscopic First Derivative Method for Simultaneous Estimation of Dapagliflozin and Metformin Hydrochloride in Synthetic Mixture” J. of Bioequv. study, 2015,1(1),102.
18. Manasa S, Dhanlaxmi K, Reddy NG,Sreenivasa S. “ Development And Validation Of A Rp-Hplc Method For The Estimation Of Dapagliflozin In Api” Int. J. of Pharma sci and Res., 2014,5(12), 5394-539.
19. Jani BR , Shah KV, Kapupara PP, “Development And Validation Of UV Spectroscopic Method For Simultaneous Estimation Of Dapagliflozin And Metformin Hydrochloride In Synthetic Mixture” int. j. res. and Dev.pharma and life sci., 2015, 4(3),1569-1576
20. Al-Salman HNK, Ali ET, Jabir M, Sulaiman GM, Al-Jadaan SAS. 2-Benzhydrylsulfinyl-N-hydroxyacetamide-Na extracted from fig as a novel cytotoxic and apoptosis inducer in SKOV-3 and AMJ-13 cell lines via P53 and caspase-8 pathway. European Food Research and Technology 2020; 246(8): 1-18.
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