Evaluation of the Anti-Psoriatic Activity of Dapagliflozin and Levofloxacin Ointment on the Tail Mice
Main Article Content
Keywords
dapagliflozin, levofloxacin, psoriasis.
Abstract
The goal of this research study was to prepare topical ointments containing dapagliflozin and levofloxacin and evaluate their anti-psoriatic activity in an animal model. Eighty Swiss albino mice weighing between 24 and 30 g were randomly divided into eight groups of 10 mice each. The first group was the control group, which received a baseline ointment. The second group was treated with clobetasol (5%) cream for six days. The third, fourth, and fifth groups were treated with dapagliflozin ointments at concentrations of 5%, 10%, and 20%, respectively, which were daily applied. The sixth, seventh, and eighth groups were treated with levofloxacin ointments at concentrations of 10%, 20%, and 40%, respectively, which were daily applied. Histopathological changes were observed in the treated skin. The results demonstrated that dapagliflozin and levofloxacin ointments at the specified concentrations could significantly reduce the severity of symptoms such as erythema, scaling, and thickening, as well as inflammation. Therefore, these ointments have strong anti-psoriatic and anti-inflammatory properties.
References
1. Abdollahi, E., Keyhanfar, F., Delbandi, A. A., Falak, R., Hajimiresmaiel, S. J., & Shafiei, M. (2022). Dapagliflozin exerts anti-inflammatory effects via inhibition of LPS-induced TLR-4 overexpression and NF-κB activation in human endothelial cells and differentiated macrophages. European Journal of Pharmacology, 918, 174715.
2. Al-Saedi, H. F., Al-Zubaidy, A. A., Ramadhan, M. A., & Mohammad, H. A. (2019). Effect of
metformin gel against imiquimod induced psoriasis in mice. Int J Res Pharm Sci, 10(2), 795-802.
3. Bhatia, A., Singh, B., Wadhwa, S., Raza, K. & Katare, O. P. 2014. Novel phospholipid-based topical formulations of tamoxifen: Evaluation for antipsoriatic activity using mouse-tail model. Pharmaceutical development and technology, 19, 160-163 .
4. Bush LM, Chaparro-Rojas F, Okeh V, Etienne J. Cumulative clinical experience from over a decade of use of levofloxacin in urinary tract infections: critical appraisal and role in therapy. Infect Drug Resist. 2011;4:177-89.
5. Castro, L., Lopez, C., & Levy, A. (2020). Levofloxacin-Induced Bilateral Leg Edema: A Review of Case Reports.
6. Dhillon, S. (2019). Dapagliflozin: a review in type 2 diabetes. Drugs, 79(10), 1135-1146.
7. Flutter, B. & Nestle, F. O. 2013. TLRS to cytokines: Mechanistic insights from the imiquimod mouse model of psoriasis. Europe Journal of Immunology, 43, 3138-3146.
8. Hashim, I. I. A., El-Magd, N. F. A., El-Sheakh, A. R., Hamed, M. F. & El, A. E.-G. H. A. 2018. Pivotal role of acitretin nanovesicular gel for effective treatment of psoriasis: Ex vivo–in vivo evaluation study. International journal of nanomedicine, 13, 1059-1079.
9. He XQ, Yao Q, Hall DD, Song ZY, Fan D, You YT, et al. Levofloxacin exerts broad-spectrum anticancer activity via regulation of THBS1, LAPTM5, SRD5A3, MFAP5 and P4HA1. Anticancer Drugs. 2021;1:e235–46.
10. Jahandideh M, Kharazi P, Jafariazar Z. Preparation of a Topical Product from Allium sativum Retrieved from Iranian Traditional Medicine. Research Journal of Pharmacognosy. 2019;6(4):3-6.
11. Joshi, S. C. 2011. Sol-gel behavior of hydroxypropyl methylcellulose (HPMC) in ionic media including drug release. Materials, 4, 1861-1905.
12. Kagami, S., Rizzo, H. L., Lee, J. J., Koguchi, Y. & Blauvelt, A. 2010. Circulating Th17, Th22, and Th1 cells are increased in psoriasis. Journal of Investigative Dermatology, 130, 1373-1383.
13. Khondker, A., Bider, R. C., Passos-Gastaldo, I., Wright, G. D., & Rheinstädter, M. C. (2021). Membrane interactions of non-membrane targetingantibiotics: The case of aminoglycosides, macrolides, and fluoroquinolones. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1863(1), 183448.
14. Lakshmi, J. N., Babu, A. N., & Nadendla, R. R. (2020). Evaluation of anti-psoriatic activity of selected phytochemicals on UV-induced psoriasis in mouse tail model. Indian Journal of Physiology and Pharmacology, 64(2), 123-128.
15. Laxmi, R. J., Karthikeyan, R., Babu, P. S. & Babu, R. N. 2013. Formulation and evaluation of antipsoriatic gel using natural excipients. Journal of Acute Disease, 2, 115-121.
16. Limandjaja, G., Van Den Broek, L., Waaijman, T., Van Veen, H., Everts, V., Monstrey, S., Scheper, R., Niessen, F. & Gibbs, S. 2017. Increased epidermal thickness and abnormal epidermal differentiation in keloid scars. British Journal of Dermatology, 176,116-126.
17. Liu, L. M., Song, J. T., Gao, F., & Zhang, H. (2019). Therapeutic effect of the quinolone levofloxacin on inflammatory fibroblast-like synoviocytes. Annals of Clinical & Laboratory Science, 49(1), 9-15.
18. Luo, P., Huang, Y., Hang, X., Tong, Q., Zeng, L., Jia, J., ... & Bi, H. (2021). Dihydrotanshinone I is effective against drug-resistant Helicobacter pylori in vitro and in vivo. Antimicrobial Agents and Chemotherapy, 65(3), e01921-20.
19. Parnami, N., Garg, T., Rath, G. & Goyal, A. K. 2014. Development and characterization of nanocarriers for topical treatment of psoriasis by using combination therapy. Artificial cells, nanomedicine, and biotechnology, 42, 406-412.
20. Podder, V., & Sadiq, N. M. (2019). Levofloxacin.
21. Sebök, B., Bonnekoh, B., Kerényi, M. & Gollnick, H. 2000. Tazarotene induces epidermal cell differentiation in the mouse tail test used as an animal model for psoriasis. Skin Pharmacology and Physiology, 13, 285-291.
22. Shelleh, H. H. & Al-Hatiti, H. S. 2004. Pattern of skin diseases in a hospital in Southwestern Saudi Arabia. Saudi medical journal, 25,507-510.
23. Zhang, N., Zheng, Q., Wang, Y., Lin, J., Wang, H., Liu, R., ... & Chen, X. (2021). Renoprotective effect of the recombinant anti-IL-6R fusion proteins by inhibiting JAK2/STAT3 signaling pathway in diabetic nephropathy. Frontiers in Pharmacology, 12, 681424.
24. Zhou, J., Zhu, J., Yu, S. J., Ma, H. L., Chen, J., Ding, X. F., ... & Zhang, Q. (2020). Sodium-glucose co-transporter-2 (SGLT-2) inhibition reduces glucose uptake to induce breast cancer cell growth arrest through AMPK/mTOR pathway. Biomedicine & Pharmacotherapy, 132, 110821.
2. Al-Saedi, H. F., Al-Zubaidy, A. A., Ramadhan, M. A., & Mohammad, H. A. (2019). Effect of
metformin gel against imiquimod induced psoriasis in mice. Int J Res Pharm Sci, 10(2), 795-802.
3. Bhatia, A., Singh, B., Wadhwa, S., Raza, K. & Katare, O. P. 2014. Novel phospholipid-based topical formulations of tamoxifen: Evaluation for antipsoriatic activity using mouse-tail model. Pharmaceutical development and technology, 19, 160-163 .
4. Bush LM, Chaparro-Rojas F, Okeh V, Etienne J. Cumulative clinical experience from over a decade of use of levofloxacin in urinary tract infections: critical appraisal and role in therapy. Infect Drug Resist. 2011;4:177-89.
5. Castro, L., Lopez, C., & Levy, A. (2020). Levofloxacin-Induced Bilateral Leg Edema: A Review of Case Reports.
6. Dhillon, S. (2019). Dapagliflozin: a review in type 2 diabetes. Drugs, 79(10), 1135-1146.
7. Flutter, B. & Nestle, F. O. 2013. TLRS to cytokines: Mechanistic insights from the imiquimod mouse model of psoriasis. Europe Journal of Immunology, 43, 3138-3146.
8. Hashim, I. I. A., El-Magd, N. F. A., El-Sheakh, A. R., Hamed, M. F. & El, A. E.-G. H. A. 2018. Pivotal role of acitretin nanovesicular gel for effective treatment of psoriasis: Ex vivo–in vivo evaluation study. International journal of nanomedicine, 13, 1059-1079.
9. He XQ, Yao Q, Hall DD, Song ZY, Fan D, You YT, et al. Levofloxacin exerts broad-spectrum anticancer activity via regulation of THBS1, LAPTM5, SRD5A3, MFAP5 and P4HA1. Anticancer Drugs. 2021;1:e235–46.
10. Jahandideh M, Kharazi P, Jafariazar Z. Preparation of a Topical Product from Allium sativum Retrieved from Iranian Traditional Medicine. Research Journal of Pharmacognosy. 2019;6(4):3-6.
11. Joshi, S. C. 2011. Sol-gel behavior of hydroxypropyl methylcellulose (HPMC) in ionic media including drug release. Materials, 4, 1861-1905.
12. Kagami, S., Rizzo, H. L., Lee, J. J., Koguchi, Y. & Blauvelt, A. 2010. Circulating Th17, Th22, and Th1 cells are increased in psoriasis. Journal of Investigative Dermatology, 130, 1373-1383.
13. Khondker, A., Bider, R. C., Passos-Gastaldo, I., Wright, G. D., & Rheinstädter, M. C. (2021). Membrane interactions of non-membrane targetingantibiotics: The case of aminoglycosides, macrolides, and fluoroquinolones. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1863(1), 183448.
14. Lakshmi, J. N., Babu, A. N., & Nadendla, R. R. (2020). Evaluation of anti-psoriatic activity of selected phytochemicals on UV-induced psoriasis in mouse tail model. Indian Journal of Physiology and Pharmacology, 64(2), 123-128.
15. Laxmi, R. J., Karthikeyan, R., Babu, P. S. & Babu, R. N. 2013. Formulation and evaluation of antipsoriatic gel using natural excipients. Journal of Acute Disease, 2, 115-121.
16. Limandjaja, G., Van Den Broek, L., Waaijman, T., Van Veen, H., Everts, V., Monstrey, S., Scheper, R., Niessen, F. & Gibbs, S. 2017. Increased epidermal thickness and abnormal epidermal differentiation in keloid scars. British Journal of Dermatology, 176,116-126.
17. Liu, L. M., Song, J. T., Gao, F., & Zhang, H. (2019). Therapeutic effect of the quinolone levofloxacin on inflammatory fibroblast-like synoviocytes. Annals of Clinical & Laboratory Science, 49(1), 9-15.
18. Luo, P., Huang, Y., Hang, X., Tong, Q., Zeng, L., Jia, J., ... & Bi, H. (2021). Dihydrotanshinone I is effective against drug-resistant Helicobacter pylori in vitro and in vivo. Antimicrobial Agents and Chemotherapy, 65(3), e01921-20.
19. Parnami, N., Garg, T., Rath, G. & Goyal, A. K. 2014. Development and characterization of nanocarriers for topical treatment of psoriasis by using combination therapy. Artificial cells, nanomedicine, and biotechnology, 42, 406-412.
20. Podder, V., & Sadiq, N. M. (2019). Levofloxacin.
21. Sebök, B., Bonnekoh, B., Kerényi, M. & Gollnick, H. 2000. Tazarotene induces epidermal cell differentiation in the mouse tail test used as an animal model for psoriasis. Skin Pharmacology and Physiology, 13, 285-291.
22. Shelleh, H. H. & Al-Hatiti, H. S. 2004. Pattern of skin diseases in a hospital in Southwestern Saudi Arabia. Saudi medical journal, 25,507-510.
23. Zhang, N., Zheng, Q., Wang, Y., Lin, J., Wang, H., Liu, R., ... & Chen, X. (2021). Renoprotective effect of the recombinant anti-IL-6R fusion proteins by inhibiting JAK2/STAT3 signaling pathway in diabetic nephropathy. Frontiers in Pharmacology, 12, 681424.
24. Zhou, J., Zhu, J., Yu, S. J., Ma, H. L., Chen, J., Ding, X. F., ... & Zhang, Q. (2020). Sodium-glucose co-transporter-2 (SGLT-2) inhibition reduces glucose uptake to induce breast cancer cell growth arrest through AMPK/mTOR pathway. Biomedicine & Pharmacotherapy, 132, 110821.
Article Sidebar
Published
Jun 4, 2023
Article Details
How to Cite
Waleed Khaled Younis Al bahadly, Ahlem Bdioui, Moaed E. Al-Gazally, Haider F. Al-Saedi, Mukhallad A. Ramadhan, & Sihem Hmissa Belhaj Salah. (2023). Evaluation of the Anti-Psoriatic Activity of Dapagliflozin and Levofloxacin Ointment on the Tail Mice. Journal of Population Therapeutics and Clinical Pharmacology, 30(13), 226-235. https://doi.org/10.47750/jptcp.2023.30.13.023
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Waleed Khaled Younis Al bahadly, Ahlem Bdioui, Moaed E. Al-Gazally, Haider F. Al-Saedi, Mukhallad A. Ramadhan, & Sihem Hmissa Belhaj Salah. (2023). Evaluation of the Anti-Psoriatic Activity of Dapagliflozin and Levofloxacin Ointment on the Tail Mice. Journal of Population Therapeutics and Clinical Pharmacology, 30(13), 226-235. https://doi.org/10.47750/jptcp.2023.30.13.023