Identification of Aeromonas species in trout in Tunceli province by MALDI-TOF MS method
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Keywords
Aeromonas species, rainbow trout, 16S rDNA, MALDI-TOF MS, gyrB
Abstract
The genus Aeromonas has undergone a number of taxonomic and nomenclature revisions over the past 20 years, and new (sub)species and biogroups are continuously described. The aim of this study was to determine the prevalence of Aeromonas species in freshwater fish farms and factors affecting their prevalence in Tunceli province. In addition, it was aimed to compare the different methods such as MALDI-TOF MS, 16S rDNA sequencing and housekeeping gene sequencing (gyrB), method for species class identification of Aeromonas. In our study, tissue samples taken from the intestine, kidney, spleen and liver of 200 juvenile and adult rainbow trout randomly selected from the farms in Mazgirt, Çemişgezek, Ovacık, Pertek districts of Tunceli province in March-May and November-January were used. In addition, 64 water samples taken from net cages and concrete pools of the same facilities were used. Five species of Aeromonas were more prevalent (MP) in fish farms, A. sobria (47.52%) was the highest, followed by A. veronii (26.95%), A. media (12.06%) and A. hydrophila (7.80%). The less prevalent species were A. salmonicida (5.67%). In our study, the accuracy of MALDI-TOF MS results of 141 isolates identified as Aeromonas species was found to be 100% (141 isolates) at genus level and 92.2% (130 isolates) at species level. In the phylogenetic studies of the genus Aeromonas, it was concluded that 16S rDNA and gyrB gene sequencing methods are reliable methods at the point where no distinction can be made at the species level.
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