In Vitro Biological Activity 0f Aqueous Extract of Cyanthillium Cinereum Against Oral Pathogens
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Keywords
Chronic renal failure, Renin , Aspartate Amino Transferase Alanine Amino Transferase , Albumin, Globulin, Calcium , Sodium , Potassium
Abstract
Background: Cyanthillium cinereum is used as a smoking cessation herb in Thailand as these leaves and flowers have a tiny amount of nicotine. Aim of the study is to investigate the antioxidant, anti-inflammatory, cytotoxicity and antimicrobial activity against common oral pathogens of Cyanthillium cinereum.
Methods: Aqueous extract was prepared and antioxidant activity assessed using DPPH Assay at 5 μL, 10 μL, 20 μL, 30 μL, 50 μL concentrations. Cytotoxic effect of extract was assessed using Brine Shrimp Assay at 5 μL, 10 μL, 20 μL, 40μL and 80μL concentrations, anti-inflammatory activity using Bovine Serum Albumin (BSA) assay and antimicrobial activity against oral pathogens was assessed using well diffusion method. Independent t test and ANOVA were used for data analysis.
Results: Antimicrobial activity against S. mutans was more in 100 μL (ZOI – 15.23±0.25; p value < 0.001); but there was no antifungal activity against Candida albicans; antioxidant activity of aqueous extract of Vernonia cinerea was more when compared with the standard ascorbic acid (p value < 0.05); Antiinflammatory activity was more in 50 μL of aqueous extract when compared with Diclofenac (94.16±0.76 vs 83.86±0.51). Cytotoxicity increases with increased concentration, 50% death at 5 μL and 90% death at 50 μL.
Conclusion: Cyanthillium cinereum showed good antimicrobial activity against S mutans. Cyanthillium cinereum have very less cytotoxic effects and also has a potential to serve as a good anti-inflammatory and antioxidant agent.
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