REAL TIME AND RAPID DETECTION OF NDM-1 GENE IN BETA LACTAM RESISTANT BACTERIA USING ELECTROCHEMICAL ASSAY BASED APTAGENE NANORECEPTORS
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Keywords
NDM-1 gene, aptamer binding, PCR amplification, aptasensor, single strand DNA
Abstract
Background: New Delhi Metallo-b-lactamase (NDM-1) is an enzyme that makes bacteria resistant to a broad range of beta-lactam antibiotics
Methods: In this study, the target gene was NDM-1 of beta lactam resistant bacteria which isolated on Mueller Hinton Agar (MHA) and identified via DNA extraction and PCR amplification then compared with the electrochemical assay based aptamer nanoreceptors.
Results: The results were encouraged and indicate that specific DNA aptasensor identified signal based the aptamer NDM-1 gene binding and exhibited affinity for the target DNA single strand, resulting in the direct detection of the NDM-1 in complex clinical bacteria samples in compare with PCR amplification.
Conclusions: This novel electrochemical gene sensor method allows for the cost effective, rapid, specific, and more sensitive detection of the NDM-1.
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Hisham Mohammad
College of Science, University of Basrah, Iraq
Hanan S. Makii
Basrah Children Specialty Hospital, Basrah Province, Iraq
Abbas A.AL-Hasani
College of Medicine, University of Basrah, Basra, Iraq
Muna M.AL-Hammadani
Basrah Children Specialty Hospital, Basrah Province, Iraq