Evaluation the possible Genotoxic Effect of Vitex Negundo Ethyl Acetate Fraction on Chromosomal Aberration in Mouse Spleen and Bone Marrow Cell
Main Article Content
Keywords
vitex negundo, bone marrow cells, spleen cells, ethyl acetate fraction
Abstract
Chromosome aberrations have long been recognized to be an important biomarker of human exposure to ionising radiation and genotoxic chemicals .many of plant in nature use for treatment of many diseases The present study is designed to evaluate the possible genotoxic effect of ethyl acetate fraction of vitex negundo (verbenaceae)on chromosomal integrity at two different doses (100mg/kg and 200mg/kg) on both bone marrow cells and spleen cells in mice for seven successive days, the results was compare their effects with methotrexate which was used as a positive control. The results have been showed that ethyl acetate fraction of vitex negundo at both doses showed a significant decrease in individual and total chromosomal aberration when compared with negative control (dimethylsulfoxide).
References
1. Flavio F.V. Borges1 , Thiago C. Machado1 , Assessment of the cytotoxic, genotoxic, and antigenotoxic activities of Celtis iguanaea (Jacq.) in mice Anais da Academia Brasileira de Ciências (2013) 85(3)
2. Halberstein RA. 2005. Medicinal plants: historical and crosscultural usage patterns. Annals of Epidemiology 15: 686- 699
3. Nadkarni, K.M., 1954. Indian Materia Medica with Ayurvedic Unani-Tibbi, Siddha, Allopathic, Homeopathic, Naturopathic and Home Remedies. Popular Prakashan, Mumbai.
4. Muthu, C., Ayyanar, M., Raja, N., Ignacimuthu, S., 2006. Medicinal plants used by traditional healers in Kancheepuram District of Tamil Nadu,
India. J. Ethnobiol. Ethnomed. 2, 43.
5. Alam, A.I., Gomes, A., 2003. Snake venom neutralization by Indian medicinal plants Vitex negundo and Emblica officinalis) root extracts. J. Ethnopharmacol. 86 (75–80).
6. Gunjan Guha, V. Rajkumar, R. Ashok Kumar Polyphenolic constituents of methanolic and aqueous extracts of Vitex negundo render protection to Hep3B cells against oxidative cytotoxicity Food and Chemical Toxicology 48 (2010) 2133–2138
7. Tiwari, O.P., Tripathi, Y.B., 2007. Antioxidant properties of different fractions of Vitex negundo Linn. Food Chem. 100 (3), 1170–1176.
8. Venkateswarlu K. Vitex negundo: Medicinal values, Biological activities, Toxicity studies and Phytopharmacological actions. Int. J Pharm. Phytopharmacol. Res. 2012; 2(2):126-133.
9. Ladda PL, Magdum CS. Vitex negundo Linn: Ethnobotany, phytochemistry and pharmacology-A review. International Journal of Advances in Pharmacy, Biology and Chemistry. 2012; 1(1):111-120
10. Shihab Hattab Mutlag1, Sajida Hussien Ismael1, Ali Faris Hassan*1, Ruaa Fadhil Abbas Genotoxic Effect of Ethyl acetate Fraction of Cressa cretica on Chromosomal Aberration on Bone Marrow Cells and Spleen Cells in Mice. Int. J. Pharm. Sci. Rev. Res., 43(2), March - April 2017; Article No. 39, Pages: 220-223.
11. Won K, Woo S L, Tae K Y, Hyun H S, Jung H C, ShSang W L, Sung H S. Chromosomal abnormalities spontaneousabortion after assisted reproductive treatment, BMC Medical Genetics, 11, 2010,15
12. Ali Faris Hassan Shihab Hattab Mutlag, Sajida Hussien Ismael1, Genotoxic Effect of Ethyl acetate Fraction of Cressa cretica on Chromosomal Aberration on Bone Marrow Cells and Spleen Cells in Mice. Int. J. Pharm. Sci. Rev. Res., 43(2), March - April 2017; Article No. 39, Pages: 220-223
13. Shihab Hattab Mutlag1*, Maha Noori Hamad1 , Ibrahim Salih Abbas2, Sajida Hussein Ismael 1 The Evaluation of Ethyl Acetate Fraction of Cressa cretica Effect on Mitotic Index and Micronucleous Frequency in Mice Int. J. Pharm. Sci. Rev. Res., 45(1), July - August 2017; Article No. 28, Pages: 147-150
14. Issa Al-Assaf*,1 and Mays Khazem.Phytochemical screening and Free radicals scavenging activity of leaves of Echinops polyceras Boiss. grown in Syria. Iraqi J Pharm Sci, Vol.31(1) 2022.
15. Aruoma O. Methodological considerations for characterizing potential antioxidant actions of bioactive com13-ponents in plant foods, Mutation Res, 523, 2003, 9-20
16. Sonal Shah,TusharDhanani,SatyanshuKumarn Validated HPLCmethodforidentification and quantification of p-hydroxy benzoicacidandagnuside in Vitex negundo and Vitex trifolia Journal of Pharmaceutical Analysis 2013(500-508).
17. Amal khan,sadia naz, Dovepress Bioactive chromone constituents from Vitex negundo alleviate pain and inflammation Journal of Pain Research 2018( 95-102)
18. Fatemeh Babaei1,Armita Moafizad2 , and Zahra Darvishv. Review of the effects of vitexin in oxidative stress-related diseases.Food Sci Nutr. 2020;8:2569–2580
19. Sarah Saad Hasson *,1, Ibrahim Saleh Abbas Isolation of beta-sitosterol and evaluation of antioxidant Activity of Iraqi Campsis grandiflora flowers Iraqi J Pharm Sci, Vol.31(1) 2022.
20. Issa Al-Assaf*,1 and Mays Khazem Phytochemical screening and Free radicals scavenging activity of leaves of Echinops polyceras Boiss. grown in Syria. Iraqi J Pharm Sci, Vol.31(1) 2022
2. Halberstein RA. 2005. Medicinal plants: historical and crosscultural usage patterns. Annals of Epidemiology 15: 686- 699
3. Nadkarni, K.M., 1954. Indian Materia Medica with Ayurvedic Unani-Tibbi, Siddha, Allopathic, Homeopathic, Naturopathic and Home Remedies. Popular Prakashan, Mumbai.
4. Muthu, C., Ayyanar, M., Raja, N., Ignacimuthu, S., 2006. Medicinal plants used by traditional healers in Kancheepuram District of Tamil Nadu,
India. J. Ethnobiol. Ethnomed. 2, 43.
5. Alam, A.I., Gomes, A., 2003. Snake venom neutralization by Indian medicinal plants Vitex negundo and Emblica officinalis) root extracts. J. Ethnopharmacol. 86 (75–80).
6. Gunjan Guha, V. Rajkumar, R. Ashok Kumar Polyphenolic constituents of methanolic and aqueous extracts of Vitex negundo render protection to Hep3B cells against oxidative cytotoxicity Food and Chemical Toxicology 48 (2010) 2133–2138
7. Tiwari, O.P., Tripathi, Y.B., 2007. Antioxidant properties of different fractions of Vitex negundo Linn. Food Chem. 100 (3), 1170–1176.
8. Venkateswarlu K. Vitex negundo: Medicinal values, Biological activities, Toxicity studies and Phytopharmacological actions. Int. J Pharm. Phytopharmacol. Res. 2012; 2(2):126-133.
9. Ladda PL, Magdum CS. Vitex negundo Linn: Ethnobotany, phytochemistry and pharmacology-A review. International Journal of Advances in Pharmacy, Biology and Chemistry. 2012; 1(1):111-120
10. Shihab Hattab Mutlag1, Sajida Hussien Ismael1, Ali Faris Hassan*1, Ruaa Fadhil Abbas Genotoxic Effect of Ethyl acetate Fraction of Cressa cretica on Chromosomal Aberration on Bone Marrow Cells and Spleen Cells in Mice. Int. J. Pharm. Sci. Rev. Res., 43(2), March - April 2017; Article No. 39, Pages: 220-223.
11. Won K, Woo S L, Tae K Y, Hyun H S, Jung H C, ShSang W L, Sung H S. Chromosomal abnormalities spontaneousabortion after assisted reproductive treatment, BMC Medical Genetics, 11, 2010,15
12. Ali Faris Hassan Shihab Hattab Mutlag, Sajida Hussien Ismael1, Genotoxic Effect of Ethyl acetate Fraction of Cressa cretica on Chromosomal Aberration on Bone Marrow Cells and Spleen Cells in Mice. Int. J. Pharm. Sci. Rev. Res., 43(2), March - April 2017; Article No. 39, Pages: 220-223
13. Shihab Hattab Mutlag1*, Maha Noori Hamad1 , Ibrahim Salih Abbas2, Sajida Hussein Ismael 1 The Evaluation of Ethyl Acetate Fraction of Cressa cretica Effect on Mitotic Index and Micronucleous Frequency in Mice Int. J. Pharm. Sci. Rev. Res., 45(1), July - August 2017; Article No. 28, Pages: 147-150
14. Issa Al-Assaf*,1 and Mays Khazem.Phytochemical screening and Free radicals scavenging activity of leaves of Echinops polyceras Boiss. grown in Syria. Iraqi J Pharm Sci, Vol.31(1) 2022.
15. Aruoma O. Methodological considerations for characterizing potential antioxidant actions of bioactive com13-ponents in plant foods, Mutation Res, 523, 2003, 9-20
16. Sonal Shah,TusharDhanani,SatyanshuKumarn Validated HPLCmethodforidentification and quantification of p-hydroxy benzoicacidandagnuside in Vitex negundo and Vitex trifolia Journal of Pharmaceutical Analysis 2013(500-508).
17. Amal khan,sadia naz, Dovepress Bioactive chromone constituents from Vitex negundo alleviate pain and inflammation Journal of Pain Research 2018( 95-102)
18. Fatemeh Babaei1,Armita Moafizad2 , and Zahra Darvishv. Review of the effects of vitexin in oxidative stress-related diseases.Food Sci Nutr. 2020;8:2569–2580
19. Sarah Saad Hasson *,1, Ibrahim Saleh Abbas Isolation of beta-sitosterol and evaluation of antioxidant Activity of Iraqi Campsis grandiflora flowers Iraqi J Pharm Sci, Vol.31(1) 2022.
20. Issa Al-Assaf*,1 and Mays Khazem Phytochemical screening and Free radicals scavenging activity of leaves of Echinops polyceras Boiss. grown in Syria. Iraqi J Pharm Sci, Vol.31(1) 2022
Article Sidebar
Published
Mar 29, 2023
Article Details
How to Cite
Haider mohammed jabber, & Shihab Hattab Mutlag. (2023). Evaluation the possible Genotoxic Effect of Vitex Negundo Ethyl Acetate Fraction on Chromosomal Aberration in Mouse Spleen and Bone Marrow Cell. Journal of Population Therapeutics and Clinical Pharmacology, 30(5), 288-294. https://doi.org/10.47750/jptcp.2023.30.05.029
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Haider mohammed jabber, & Shihab Hattab Mutlag. (2023). Evaluation the possible Genotoxic Effect of Vitex Negundo Ethyl Acetate Fraction on Chromosomal Aberration in Mouse Spleen and Bone Marrow Cell. Journal of Population Therapeutics and Clinical Pharmacology, 30(5), 288-294. https://doi.org/10.47750/jptcp.2023.30.05.029