Evolution of cytotoxicity of the phytopigments Isolated from Spirulina platen sis using MTT assay
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Keywords
Anticancer; Monkey Kidney Epithelial Cells; Human breast cancer; Human colon cancer; MTT assay
Abstract
Phytopigments of Spirulina platen sis extract has anticancer activity against various types of cancer cell cultures. However, study about effect of phytopigments on Monkey Kidney Epithelial Cells (Vero Line), Human breast cancer (MCF-7) and Human Colon Cancer-(HT-29)cell lines. This study aimed to reveal the anticancer activity of phytopigments from Spirulina platensis extract on Vero line, MCF7 and HT-29 cells. The research was an in vitro experimental study, with the investigation on cytotoxicity as the anticancer parameters. cytotoxicity test was conducted through MTT assay to observe the visualization and inhibition of proliferation of different concentrations of phytopigments like Zeaxanthin, Phycocyanin, β-Carotene, Phycoerythrin, Chlorophyll-a, and Chlorophyll-b in several incubation times on the cancer cell line. The obtained data were then processed statistically with the Two-Way ANOVA test at a significance value of p <0.05. Based on the results, it could be postulated that in all pigments the phycocyanin shows better activity compare with other at IC50on Vero at152.2 ± 0.20, MCF-7 at 22.60 ± 0.30, HT-29 at 23.30 ± 0.32 compare with standard doxorubicin.
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