Propolis flavonoids liposome ameliorate doxorubicin-induced cardiotoxicity in rats
Main Article Content
Keywords
Propolis flavonoids liposome, Doxorubicin, cardiotoxicity
Abstract
The clinical applications of doxorubicin (DOX), a potent anthracycline antineoplastic drug, is often restricted by its deleterious cardiotoxic effects. Thus, for improving usage of DOX, the aim of this study was to assess the cardio-protective effects of nanopropolis in a rat model of DOX-induced acute cardiotoxicity. Through maceration method for 7 days in ethanol then 7 days in absolute ethyl acetate, flavonoidal propolis extract was obtained. twenty albino Rats were divided in to four equal groups: Control rats that received BPS for 10 days, DOX-treated rats that recived a single I.P injection of DOX (25 mg/kg BW) on day 7 from beginning of experiment. Rats received liposome orally for 10 consecutive days and single dose of doxorubicin (25 mg/kg BW IP) on day 7 from beginning of
experiment. Rats groups received propolis liposome formulas orally for 10 consecutive days and single dose of doxorubicin (25 mg/kg BW IP) on day 7 from beginning of experiment. Sera were collected for screening of cardiac injury markers.The administration of DOX in single dose showed high cardiac biomarkers CK-MB, Troponin I, and LDH than in the negative group. Treatment with propolis
nanoformulas shown ameliorated the DOX‑induced cardiac tissue damage by decline the serum levels of CK-MB, Troponin I, and Lactate dehydrogenase.
The experiment parts endorsed conclusion that nanopropolis may exhibit remarkable protective effect in DOX‑induced cardiac toxicity through improve a number of cardiac biomarkers linked with cardiac toxicity, and may be represent a potential agents for attenuation and prevention of the serious complications accompanied with DOX in clinical practice.
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29. Mouse, H. A., Tilaoui, M., Jaafari, A., M’barek,L. A., Aboufatima, R., Chait, A., & Zyad, A. (2012). Evaluation of the in vitro and in vivo
anticancer properties of Moroccan propolis extracts. Revista Brasileira de Farmacognosia, 22(3), 558–567.
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32. Singh, A. Ray, A. Mishra, R. Biswal, P. Yadar, R. and Ghatuary, K. (2020). Phyto-Phospholipid Complexes: Innovative Approach to Enhance theBioavailability and Therapeutic Efficacy of Herbal Extract. Pharmaceutical and Biosciences Journal 8: 01-09.
33. Babazadeh, A. Zeinali, M. and Hamishehkar, H. (2018). NanoPhytosome: A developing platform for herbal anti-cancer agents in cancer therapy. Current Drug Targets, 19: 170-180.
34. Nathalie Chahine, Jean Hanna, Hassane Makhlouf, Laurent Duca, Laurent Martiny & Ramez Chahine (2013). Protective effect of
saffron extract against doxorubicin cardiotoxicity in isolated rabbit heart, Pharmaceutical Biology, 51:12, 1564-1571.
Perspectives in Drug Discovery and Alternative Therapy. Molecules, 26(4), 1109-1139.
2. Lipshultz SE, Franco VI, and Miller TL.(2018). Cardiovascular disease in adult survivors of childhood cancer. Annu Rev Med; 66:161–176.
3. Force T, Wang Y. (2013). Mechanism-based engineering against anthracycline cardiotoxicity. Circulation. 128: 98–100.
4. Fadillioglu, E., Oztas, E., Erdogan, H.,Yagmurca, M., Sogut, S., Ucar, M., & Irmak, M. K. (2004). Protective effects of caffeic acid phenethyl ester on doxorubicin-induced cardiotoxicity in rats. Journal of Applied Toxicology, 24(1), 47– 52.
5. Anjum, S. I., Ullah, A., Khan, K. A., Attaullah, M., Khan, H., Ali, H., Dash, C. K. (2018). Composition and functional properties of propolis (bee glue): A review. Saudi Journal of Biological Sciences.
6. Zidan, Omar Helmi. (2020). PEGylated chitosan / doxorubicin nanoparticles and conjugated with monoclonal antibodies for breast cancer therapy. American University in Cairo, Master's Thesis. AUC Knowledge Fountain.
7. Yuan, Y., Cai, T., Xia, X., Zhang, R., Chiba, P., & Cai, Y. (2016). Nanoparticle delivery of anticancer drugs overcomes multidrug resistance
in breast cancer. Drug Delivery, 23(9), 3350– 3357.
8. Al-mohana, Al-Mohana, A.(2004). A study of activity of the local alcoholic propolis extract in the treatment of the external wounds that
experimentally infected with some pathogenic bacteria and fungi in mice.Master thesis. college of veterinary medicine at the university of
Baghdad.
9. Boufadi, Y., Soubhye, J., Riazi, A., Rousseau, A., Vanhaeverbeek, M., Nève, J., Van Antwerpen, P. (2014). Characterization and Antioxidant
Properties of Six Algerian Propolis Extracts: Ethyl Acetate Extracts Inhibit Myeloperoxidase Activity. International Journal of Molecular
Sciences, 15(2), 2327–2345.
10. Ramana, M.V. Chaudhari, A.D. Himaja, M. Satyanarayana, D. and Dua, K. (2017). Anapproach to minimize Pseudomembranous colitis
caused by clindamycin through liposomal formulation. Indian Journal of Pharmaceutical Sciences 69: 384-389.
11. Barsola, B and Kumari, P. (2022). Green synthesis of nano-propolis and nanoparticles (Se and Ag) from ethanolic extract of propolis, their
biochemical characterization: A review" Green Processing and Synthesis, vol. 11, no. 1, pp. 659-673.
12. Miyataka, H. Nishlkl, M. Matsumoto, H. FujImoto, T. Matsuka, M. and Satoh, T. (1997). Evaluation of Brazilian and Chinese Propolis by
Enzymatic and Physico-Chemical Methods.Biological & Pharmaceutical Bulletin. 20: 496-501.
13. Izgü F, Bayram G, Tosun K, İzgü D. (2017). Stratum corneum lipid liposome-encapsulated panomycocin: preparation, characterization, and
the determination of antimycotic efficacy against Candida spp. isolated from patients with vulvovaginitis in an in vitro human vaginal
epithelium tissue model. Int J Nanomedicine.
14. Ahmed AZ, Satyam SM, Shetty P, D'Souza MR.(2021). Methyl Gallate Attenuates DoxorubicinInduced Cardiotoxicity in Rats by Suppressing
Oxidative Stress. Scientifica (Cairo). 2021 Jan 13;2021:6694340.
15. Al-Rubii, A.K. (2018). Effect of Tribulus terrestris L. extract on prostatic and testicular functions of castrated rabbits. M.Sc thesis.
College of science. Baghdad University.Baghdad. Iraq.
16. Kong, A.S.-Y.; Lai, K.S.; Hee, C.-W.; Loh, J.Y.;Lim, S.H.E.; Sathiya, M. (2022). Oxidative Stress Parameters as Biomarkers of Cardiovascular
Disease towards the Development and Progression. Antioxidants, 11, 1175.
17. Abdel-Daim, M. M., kilany, O. E., Khalifa, H. A.,& Ahmed, A. A. M. (2017). Allicin ameliorates doxorubicin-induced cardiotoxicity in rats via
suppression of oxidative stress, inflammation and apoptosis. Cancer Chemotherapy and Pharmacology, 80(4), 745–753.
doi:10.1007/s00280-017-3413-7.
18. Zulhendri, F. Perera,O. Chandrasekaran,K. Ghosh, A. Tandean,S. Abdulah,R Herman,H Lesmana,R. (2022). Propolis of stingless bees for
the development of novel functional food and nutraceutical ingredients: A systematic scoping review of the experimental evidence, Journal of
Functional Foods, Volume 88
19. Rawat, P. S., Jaiswal, A., Khurana, A., Bhatti, J. S., & Navik, U. (2021). Doxorubicin-induced cardiotoxicity: An update on the molecular
mechanism and novel therapeutic strategies for effective management. Biomedicine & Pharmacotherapy, 139, 111708.
20. Chen, X., Zhang, Y., Zhu, Z., Liu, H., Gou, H., Xiong, C., Su, S. (2016). Protective effect of berberine on doxorubicin-induced acute
hepatorenal toxicity in rats. Molecular Medicine Reports, 13(5), 3953–3960.
21. Danese, E., and Montagnana, M. (2016). An historical approach to the diagnostic biomarkers of acute coronary syndrome. Annals of
Translational Medicine, 4(10), 194–194.
22. Radeva-Ilieva, M. P., Georgiev, K. D., Hvarchanova, N. R., Stoeva, S. S., Slavov, I. J., Dzhenkov, D. L., & Georgieva, M. P. (2020).
Protective Effect of Methylxanthine Fractions Isolated from Bancha Tea Leaves against Doxorubicin-Induced Cardio- and
Nephrotoxicities in Rats. BioMed Research International, 2020, 1–9.
23. Podyacheva EY, Kushnareva EA, Karpov AA and Toropova YG. (2021). Analysis of Models of Doxorubicin-Induced Cardiomyopathy in Rats
and Mice. A Modern View From the Perspective of the Pathophysiologist and the Clinician. Front. Pharmacol. 12:670479.
24. Abd Elbaky NA, A.A. Ali and Ahmed R.A, (2010). Cardioprotective Effect Of Simvastatin On Doxorubicininduced Oxidative Cardiotoxicity In Rats. Journal of Basic and Applied Sciences, 6, (1): 29-38.
25. Singal, P. Li, T. Kumar, D. Danelisen, I. and Iliskovic, N.(2000). Adriamycin-induced heart failure: mechanisms and modulation,” Molecular
and Cellular Biochemistry, vol. 207, no. 1-2, pp.77–86.
26. Billingham, M.E. J.W. Mason, M.R. Bristow and J.R. Daniels, (1998). Anthracycline cardiomyopathy monitored by morphologic
changes. Cancer Treat. Rep., 62:865-872.
27. Swain, S. Whaley, F. Ewer, M. (2003). Congestive heart failure in patients treated with doxorubicin,” Cancer, vol. 97, no. 11, pp. 2869–
2879.
28. Kong, A.S.-Y.; Lai, K.S.; Hee, C.-W.; Loh, J.Y.; Lim, S.H.E.; Sathiya, M. (2022). Oxidative Stress Parameters as Biomarkers of Cardiovascular
Disease towards the Development and Progression. Antioxidants, 11, 1175.
29. Mouse, H. A., Tilaoui, M., Jaafari, A., M’barek,L. A., Aboufatima, R., Chait, A., & Zyad, A. (2012). Evaluation of the in vitro and in vivo
anticancer properties of Moroccan propolis extracts. Revista Brasileira de Farmacognosia, 22(3), 558–567.
30. Forma E. and Bryś,M. (2021). Anticancer activity of propolis and its compounds,” Nutrients, vol. 13, no. 8, p. 2594.
31. Chopra, S., Pillai, K. K., Husain, S. Z., & Girl, D.K. (1998). Propolis Protects against DoxorubicinInduced Myocardiopathy in Rats. Experimental
and Molecular Pathology, 62(3), 190–198.
32. Singh, A. Ray, A. Mishra, R. Biswal, P. Yadar, R. and Ghatuary, K. (2020). Phyto-Phospholipid Complexes: Innovative Approach to Enhance theBioavailability and Therapeutic Efficacy of Herbal Extract. Pharmaceutical and Biosciences Journal 8: 01-09.
33. Babazadeh, A. Zeinali, M. and Hamishehkar, H. (2018). NanoPhytosome: A developing platform for herbal anti-cancer agents in cancer therapy. Current Drug Targets, 19: 170-180.
34. Nathalie Chahine, Jean Hanna, Hassane Makhlouf, Laurent Duca, Laurent Martiny & Ramez Chahine (2013). Protective effect of
saffron extract against doxorubicin cardiotoxicity in isolated rabbit heart, Pharmaceutical Biology, 51:12, 1564-1571.
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Mar 26, 2023
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How to Cite
Ali M. Ghazi, & Sijad M. Latif. (2023). Propolis flavonoids liposome ameliorate doxorubicin-induced cardiotoxicity in rats. Journal of Population Therapeutics and Clinical Pharmacology, 30(4), 230-240. https://doi.org/10.47750/jptcp.2023.30.04.022
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How to Cite
Ali M. Ghazi, & Sijad M. Latif. (2023). Propolis flavonoids liposome ameliorate doxorubicin-induced cardiotoxicity in rats. Journal of Population Therapeutics and Clinical Pharmacology, 30(4), 230-240. https://doi.org/10.47750/jptcp.2023.30.04.022