Association of S. epidermidis infection and TNF-α gene polymorphism of acne patients in Thi-Qar province/ Iraq
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Keywords
S. epidermidis, polymorphism
Abstract
The present study was aimed to identication of S. epidermidis from acne patients, and related with the genetic polymorphism of TNF-α gene in the same patients. The study were included 150 acne specimens from acne patients; The patients were diagnosed clinically by a dermatologist. As well, 75 venous blood samples were collected from same patients; and 25 samples from healthy persons as control in private clinical lab of Thi-Qar province/Iraq, during August-September, 2022. Only 60 isolates (40%) were identified as S. epidermidis. The identification of S. epidermidis isolates done by microscopic examination, morphological characterization on MSA, blood agar and chrom agar, biochemical test, then accurate identification with Viteks system. The results of genetic polymorphism of 30 samples (20: acne patients, 10: control) showed statistically non-associated between C302T SNP and acne disease. Besides, the rs1799724, C302T, three polymorphic patterns were detected, CC, CT and TT. The homozygous CC pattern was detected in the majority of samples in cases (16/20) and controls (7/10). Unusually, the homozygous TT status was not detected in both cases and control. Along, the sequencing chromatogram detected delation mutation (A nucleotide) in the position 309A of 12 studied samples (5: control, 7: patients) in comparsion with reference sequencing. Also, the existing results of allele frequency of TNF-α gene, and the odd ratio (OR= 1.5) showed that in case of the 302 C/T SNP polymorphism was risk indicator of developing acne vulgaris; so the risk more increased in the homozygote wild type (CC)
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