Purification of alkaline phosphatase and measurement of some antioxidants in the serum of patients with high blood cholesterol
Main Article Content
Keywords
ALP, Hypercolestrolemia ,Antioxadinat
Abstract
This study included the purification of the alkaline phosphatase and the estimation of the level of antioxidants in the serum of people .Those with high cholesterol. 33 blood samples were collected from people with high cholesterol in the blood of both sexes, with ages ranging between (60-42 years), and 20 blood samples were collected from people with normal cholesterol levels for both sexes.
The alkaline phosphatase was also purified, as the degree of purification of the enzyme in the purification stage reached 2.7 times, with an enzymatic yield of 42.2%, and then using the gel filtration chromatography method using Sephadex G100 gel, as a single peak was obtained for the purified enzyme as ,with a degree of purification that reached 3.8 times and an enzymatic yield of 22%. High cholesterol leads to cardiovascular disease. The results a Significat increase in activity of ALP ,Cholestrol ,Lipoprotiens, catales,uric acid.
The decrease total protie and Glutathion
References
1. D W Moss, Perspectives in Alkaline Phosphatase Research .Clinical Chemistry, Volume 38, Issue 12, 1 December 1992, Pages 2486–
2492, https://doi.org/10.1093/clinchem/38.12.24 86
2. R. J. WIEME AND L. DEMEULENAERE . Enzyme assays in liver disease. J. clin. Path.,(Ass. Clin. Path.), 4, 51-59 J Clin Pathol: first published as 10.1136/jcp.s1-4.1.51 on 1 January1970
3. Leo L. Stolbach M.D, Melvin J. Krant M.D. Norma I. Inglis B.A. William H. Fishman Ph.D. Correlation of Serum l-Phenylalanine-Sensitive
Alkaline Phosphatase, Derived From Intestine, with the Abo Blood Group of Cirrhotics.Gastroenterology ,Volume 52, Issue 5, May 1967, Pages 819-827
4. D.W. Moss , L.G. Whitby.A simplified heatinactivation method for investigating alkaline phosphatase isoenzymes in serum. Clinica Chimica Acta,Volume 61, Issue 1, 15 May 1975, Pages 63-7.
5. Christopher P Price . Multiple forms of human serum alkaline phosphatase: detection and quantitation. Ann clin Biochem 1993; 30: 355-372
6. Radaelli G, Sausen G, Cesa CC, Portal VL, Pellanda LC. Secondary Dyslipidemia In Obese Children - Is There Evidence For Pharmacological Treatment? Arq Bras Cardiol. 2018 Sep;111(3):356-361.
7. Zawacki AW, Dodge A, Woo KM, Ralphe JC, Peterson AL. In pediatric familial hypercholesterolemia, lipoprotein(a) is more predictive than LDL-C for early onset of cardiovascular disease in family members. J Clin Lipidol. 2018 Nov - Dec;12(6):1445-1451.
8. Dainis AM, Ashley EA. Cardiovascular Precision Medicine in the Genomics Era. JACC Basic Transl Sci. 2018 Apr;3(2):313-326
9. Sturm AC, Knowles JW, Gidding SS, Ahmad ZS, Ahmed CD , et al . Convened by the Familial Hypercholesterolemia Foundation. Clinical
Genetic Testing for Familial Hypercholesterolemia: JACC Scientific Expert Panel. J Am Coll Cardiol. 2018 Aug 07;72(6):662-680.
10. S. Kostadinova , M. Marhova . Purification and Properties of Alkaline Phosphatase from Bacillus Cereus. Biotechnology & Biotechnological Equipment. 24:sup1, 602-606, DOI:10.1080/13102818.2010.10817906 .2010
11. Kind, P.R. and King, E.G. (1954). "Estimation of plasma phosphatase by determination of hydrolysed phenol with amino antipyrine". J. Clin. Path.; 7: 322 - 326. Stanley , T.David , T.and Hawerds , S. Method in Enzymology . Vol.6.Vitamins and Coenzymes Part D(1979).
Sedlak , J.and Lindsay , R.H.(1968) , "Analytical biochemistry "p.192. cited by Al- Zamely (2001).
12. Khalaf ,Sarab Dalaf . Shaker, Sheerin Farok .Effect of smoking on lipid profile and biochemical Parameters in serum men smokers. . Journal of Pure Sciences ,Anbar University. Volu 3, Issue 9 .215.
13. Aswath N. Balakrishnan C. Estimation of serum, salivary immunoglobulin G, immunoglobulin A levels and total protein, hemoglobin in smokeless tobacco chewers and oral submucous fibrosis patients. Contemp Clin Dent 2015;6:157-62.
14. Guan .Yuqi, Zheng. Xun Zhou, Ying Xu. Efficacy of different urinary uric acid indicators in patients with chronic kidney disease Haochen .Guan et al. BMC Nephrology (2020) 21:290 https://doi.org/10.1186/s12882-020-01953-z
15. Kumar , V., Abbas , A .K., Fausto, N. and Aster , J .(2009)."Robbins and cotran pathologic basis of disease ".18th ed .
16. Naveed , N., Tayyib , M., Farooq , M., Riaz, R.M., Ditta, A. and Anwar, S.(2009)."Biochemical evaluation of effects of diabetes mellitus on excretory function of liver".Pakistan Journal of Medical and Health sciences ;3(1).
17. Koimeda, Y.and Inaba, M.(2002)."Diabetic Osteoporosis" Nippon Rinsho;60:459-467
18. Garcia-Martin,A.,Rozas-Moreno,P., ReyesGarcia ,R., Moralas-Santana,S. ,Garcia-Fontama, B., Garcia-Salcedo, J. A. and Munoz-Torres,
M.(2011)." Circulating levels of Sclerostin are increased in Patients with type2 diabetes mellitus".JCEM;jc:2011- 2186.
19. Mahdi, E. M., & Mustafa, M. A. (2022). Effect of different concentrations of extract of Urtica dioica and Cladosporium cladosporiodes on Tribolium castaneum or: Coleoptera after 24-48 hours of exposure in Samarra City/Iraq. HIV Nursing, 22(2), 3207-3210.
20. Mustafa, M. A., Kadham, S. M., Abbass, N. K., Karupusamy, S., Jasim, H. Y., Alreda, B. A., ... & Ahmed, M. T. (2023). A novel fuzzy M-transform technique for sustainable ground water level prediction. Applied Geomatics, 1-7.
21. Kadham, S. M., Mustafa, M. A., Abbass, N. K., & Karupusamy, S. (2022). IoT and artificial intelligence–based fuzzy-integral N-transform for sustainable groundwater management. Applied Geomatics, 1-8.
2492, https://doi.org/10.1093/clinchem/38.12.24 86
2. R. J. WIEME AND L. DEMEULENAERE . Enzyme assays in liver disease. J. clin. Path.,(Ass. Clin. Path.), 4, 51-59 J Clin Pathol: first published as 10.1136/jcp.s1-4.1.51 on 1 January1970
3. Leo L. Stolbach M.D, Melvin J. Krant M.D. Norma I. Inglis B.A. William H. Fishman Ph.D. Correlation of Serum l-Phenylalanine-Sensitive
Alkaline Phosphatase, Derived From Intestine, with the Abo Blood Group of Cirrhotics.Gastroenterology ,Volume 52, Issue 5, May 1967, Pages 819-827
4. D.W. Moss , L.G. Whitby.A simplified heatinactivation method for investigating alkaline phosphatase isoenzymes in serum. Clinica Chimica Acta,Volume 61, Issue 1, 15 May 1975, Pages 63-7.
5. Christopher P Price . Multiple forms of human serum alkaline phosphatase: detection and quantitation. Ann clin Biochem 1993; 30: 355-372
6. Radaelli G, Sausen G, Cesa CC, Portal VL, Pellanda LC. Secondary Dyslipidemia In Obese Children - Is There Evidence For Pharmacological Treatment? Arq Bras Cardiol. 2018 Sep;111(3):356-361.
7. Zawacki AW, Dodge A, Woo KM, Ralphe JC, Peterson AL. In pediatric familial hypercholesterolemia, lipoprotein(a) is more predictive than LDL-C for early onset of cardiovascular disease in family members. J Clin Lipidol. 2018 Nov - Dec;12(6):1445-1451.
8. Dainis AM, Ashley EA. Cardiovascular Precision Medicine in the Genomics Era. JACC Basic Transl Sci. 2018 Apr;3(2):313-326
9. Sturm AC, Knowles JW, Gidding SS, Ahmad ZS, Ahmed CD , et al . Convened by the Familial Hypercholesterolemia Foundation. Clinical
Genetic Testing for Familial Hypercholesterolemia: JACC Scientific Expert Panel. J Am Coll Cardiol. 2018 Aug 07;72(6):662-680.
10. S. Kostadinova , M. Marhova . Purification and Properties of Alkaline Phosphatase from Bacillus Cereus. Biotechnology & Biotechnological Equipment. 24:sup1, 602-606, DOI:10.1080/13102818.2010.10817906 .2010
11. Kind, P.R. and King, E.G. (1954). "Estimation of plasma phosphatase by determination of hydrolysed phenol with amino antipyrine". J. Clin. Path.; 7: 322 - 326. Stanley , T.David , T.and Hawerds , S. Method in Enzymology . Vol.6.Vitamins and Coenzymes Part D(1979).
Sedlak , J.and Lindsay , R.H.(1968) , "Analytical biochemistry "p.192. cited by Al- Zamely (2001).
12. Khalaf ,Sarab Dalaf . Shaker, Sheerin Farok .Effect of smoking on lipid profile and biochemical Parameters in serum men smokers. . Journal of Pure Sciences ,Anbar University. Volu 3, Issue 9 .215.
13. Aswath N. Balakrishnan C. Estimation of serum, salivary immunoglobulin G, immunoglobulin A levels and total protein, hemoglobin in smokeless tobacco chewers and oral submucous fibrosis patients. Contemp Clin Dent 2015;6:157-62.
14. Guan .Yuqi, Zheng. Xun Zhou, Ying Xu. Efficacy of different urinary uric acid indicators in patients with chronic kidney disease Haochen .Guan et al. BMC Nephrology (2020) 21:290 https://doi.org/10.1186/s12882-020-01953-z
15. Kumar , V., Abbas , A .K., Fausto, N. and Aster , J .(2009)."Robbins and cotran pathologic basis of disease ".18th ed .
16. Naveed , N., Tayyib , M., Farooq , M., Riaz, R.M., Ditta, A. and Anwar, S.(2009)."Biochemical evaluation of effects of diabetes mellitus on excretory function of liver".Pakistan Journal of Medical and Health sciences ;3(1).
17. Koimeda, Y.and Inaba, M.(2002)."Diabetic Osteoporosis" Nippon Rinsho;60:459-467
18. Garcia-Martin,A.,Rozas-Moreno,P., ReyesGarcia ,R., Moralas-Santana,S. ,Garcia-Fontama, B., Garcia-Salcedo, J. A. and Munoz-Torres,
M.(2011)." Circulating levels of Sclerostin are increased in Patients with type2 diabetes mellitus".JCEM;jc:2011- 2186.
19. Mahdi, E. M., & Mustafa, M. A. (2022). Effect of different concentrations of extract of Urtica dioica and Cladosporium cladosporiodes on Tribolium castaneum or: Coleoptera after 24-48 hours of exposure in Samarra City/Iraq. HIV Nursing, 22(2), 3207-3210.
20. Mustafa, M. A., Kadham, S. M., Abbass, N. K., Karupusamy, S., Jasim, H. Y., Alreda, B. A., ... & Ahmed, M. T. (2023). A novel fuzzy M-transform technique for sustainable ground water level prediction. Applied Geomatics, 1-7.
21. Kadham, S. M., Mustafa, M. A., Abbass, N. K., & Karupusamy, S. (2022). IoT and artificial intelligence–based fuzzy-integral N-transform for sustainable groundwater management. Applied Geomatics, 1-8.
Article Sidebar
Published
Mar 13, 2023
Article Details
How to Cite
Nuha Ali Hadi al-Samarrai, Mohammed J.M.Hrwsh, Miami hussin ali, Reem Ibrahim Mahdi, & Mays subhi wasmi. (2023). Purification of alkaline phosphatase and measurement of some antioxidants in the serum of patients with high blood cholesterol. Journal of Population Therapeutics and Clinical Pharmacology, 30(3), 303-308. https://doi.org/10.47750/jptcp.2023.30.03.032
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Nuha Ali Hadi al-Samarrai, Mohammed J.M.Hrwsh, Miami hussin ali, Reem Ibrahim Mahdi, & Mays subhi wasmi. (2023). Purification of alkaline phosphatase and measurement of some antioxidants in the serum of patients with high blood cholesterol. Journal of Population Therapeutics and Clinical Pharmacology, 30(3), 303-308. https://doi.org/10.47750/jptcp.2023.30.03.032