New recording genes and genetic mutations of H. pylori genome in gastroduodenal disease patients in Basrah province-Iraq
Main Article Content
Keywords
gastroduodenal disorder, Helicobacter pylori, cagA, vacA, and new genetic mutations
Abstract
The aim of this study was to investigate the molecular finding of the most important H. pylori virulence factors cagA, vacA genotypes in gastric biopsies of patients with gastroduodenal diseases infected with Helicobacter pylori in Basrah-Iraq by using conventional PCR and sequencing technologies, to give acknowledge about the immunopathogenesis of the studied virulence factors in patients with
gastroduodenal disorder. A case-control study included 112 confirmed gastroduodenal patients and 112 healthy individuals as a control group. Data about age, gender, smoking, alcohol abuse, family history, occupation, residence, and clinical findings for all study populations were collected. The study show a significant effect of these virulence factors with an increased risk of gastroduodenal disorder
References
1. Haesebrouck, F.; Pasmans, F.; Flahou, B.; Chiers, K.; Baele, M.; Meyns, T.; Decostere, A.; and Ducatelle, R. (2009). Gastric helicobacters in domestic animals and nonhuman primates and their significance for human health. Clin. Microbiol. Rev., 22(2):202-23.
2. Oztekin, M; Birsen, Y.; Duygu, A.; and Raffaele, C. (2021). Overview of Helicobacter pylori infection: clinical features, treatment, and
nutritional aspects, Dis., 9(66):1-9.
3. Charitos, I. A.; D’Agostino, D.; Topi, S.; and Bottalico, L. (2021). 40 years of Helicobacter pylori: A revolution in biomedical thought.
Gastroenterol. Insights, 12(2):111-135.
4. Kumar S. (2016). Essentials of Microbiology. 1st ed., 299-300.
5. Boren, T. (2014). Helicobacter Pylori; Multitalented Adaptation of Binding Properties. Anticancer Research, 34(10):5840-5841.
6. Fan, L.; Li, R.; Li, H.; Zhang, J.; and Wang, L. (2018). genes in Helicobacter pylori isolated from gastric ulcer patients. 42(4):155-62.
7. Kusters, J. G.; Vliet, A. H.; and Kuipers, E. J. (2006). Pathogenesis of Helicobacter pylori infection. Clin. Microbiol. Rev., 19(3):449-490.
8. Wang, YK.; Kuo, FC.; Liu, CJ.; Wu, MC.; Shih, HY.; Wang, SSW.; Wu, JY.; Kuo, CH.; Huang, YK.; and Wu, DC. (2015). Diagnosis of
Helicobacter pylori infection: Current options and developments. World J. Gastroenterol., 21(40):11221-35.
9. Fernandez-Caso, B., Miqueleiz, A., Valdez, V. B., & Alarcon, T. (2022). Are molecular methods helpful for the diagnosis of Helicobacter pylori infection and for the prediction of its antimicrobial resistance?. Front. Microbiol, 13(August), 1-6.
10. Sulo, P; and Sipkova, B. (2021). DNA diagnostics for reliable and universal identification of Helicobacter pylori. World. J. Gastroenterol., 27(41):7100-7112.
11. Idowu, A.; Mzukwa, A.; Harrison, U.; Palamides, P.; Haas, R.; Mbao, M.; Mamdoo, R.; Bolon, J.; Jolaiya, T.; Smith, S.; Ally, R.; Clarke, A.; and Njom, H. (2019). Detection of Helicobacter pylori and its virulence genes (cagA, dupA, and vacA) among patients with gastroduodenal diseases in Chris Hani Baragwanath Academic Hospital, South Africa. Gastroenterol, 19(1):1-10.
12. El-Khlousy, M.; Rahman, E, A.; Mostafa, S.; Bassam, A.; Elgawad, W, A.; Elnasr, E, S.; Mohey, M.; and Ghaith, D, (2016). Study of the clinical relevance of Helicobacter pylori virulence genes to gastric diseases among Egyptian patients, Arab J. Gastroenterol., 17(2):90-94.
13. Wilkinson, D. J.; Dickins, B.; Robinson, K.; Winter J. (2022). Genomic diversity of Helicobacter pylori populations from different regions of the human stomach, Gut Microbes, 14(8):1-19.
14. Marques, A. T.; Vitor, J. M. B.; Santos, A.; Oleastro, M.; and Vale, F. F. (2020). Trends in Helicobacter pylori resistance to clarithromycin: From phenotypic to genomic approaches. Microbial Genomics, 6(3):1-11.
15. Vital, J. S.; Tanoeiro, L.; Lopes-Oliveira, R.; and Vale, F. F. (2022). Biomarker characterization and prediction of virulence and antibiotic
resistance from Helicobacter pylori next generation sequencing data. Biomolecules, 12(5):1-26.
16. Suzuki, R.; Satou, K.; Shiroma, A.; Shimoji, M.; Teruya, K.; Matsumoto, T.; Akada, J.; Hirano, T.; and Yamaoka, Y. (2019). Genome-wide mutation analysis of Helicobacter pylori after inoculation to Mongolian gerbils. Gut Pathogens, 11(1):4-9.
17. Vianna, J. S.; Ramis, I. B.; Ramos, D. F.; Gastal, O. L.; Azevedo, R.; Goncalves, C. V.; and Eduardo, P. (2018). The interplay between
mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori, Rev. Inst. Med. Trop. Sao Paulo, 60(25):1-5.
18. Jabar, S. A., & Alfaisal, A. H. M. (2017). The correlation between KRAS mutations and H. pylori in gastric cancer patients. Iraqi J.
Biotechnol., 16(3):82-93.
19. Dai, J., Zhao, J., Mao, L., Hu, Y. U. E., & Lv, B. I. N. (2022). Study on the value of antibiotic‑resistant gene detection in Helicobacter
pylori in China. Experiment. Therap. Med. 23(228):1-10.
20. Aziz, S.; Rasheed, F.; Akhter, T. S.; Zahra, R.; & Konig, S. (2022). Microbial Proteins in Stomach Biopsies Associated with Gastritis, Ulcer, and Gastric Cancer. Molecules, 27(17):1-13.
21. Jin, F.; and Yang, H. (2022). Complete Genome Sequence of Helicobacter pylori Strain 3192, Isolated from a Chinese Patient with Chronic Nonatrophic. Microbiol. Res. Announc., 11(7):0- 11.
22. Jia, X.; Huang, Q.; Lin, M.; and Chu, Y. (2022). Revealing the novel effect of Jinghua Weikang capsule against the antibiotic resistance of Helicobacter pylori. Front. Microbiol., 1-15.
23. Mohammed, H. A., & Obaid, Z. H. (2022). Pathogenesis of Helicobacter pylori: An Overview of Bacterial Virulence Agents and the
Mechanism of Occurrence Disease. J. Uni. Babyl. Pure Appli. Sci., 3(1):47-53.
24. Saber, F. omer, & Ali, M. K. (2022). Isolation and Identification of H. pylori among Iraq patients with chronic gastric inflammation. J. Faculty. Med. Baghdad, 64(2):102-108.
25. Hamed, A. T.; and Baqer, AA. B. (2022). Review on stimulations of the spread of Helicobacter pylori infection in Iraq. Inter. J. Appli. Sci. Technol., 3(6):146-156.
2. Oztekin, M; Birsen, Y.; Duygu, A.; and Raffaele, C. (2021). Overview of Helicobacter pylori infection: clinical features, treatment, and
nutritional aspects, Dis., 9(66):1-9.
3. Charitos, I. A.; D’Agostino, D.; Topi, S.; and Bottalico, L. (2021). 40 years of Helicobacter pylori: A revolution in biomedical thought.
Gastroenterol. Insights, 12(2):111-135.
4. Kumar S. (2016). Essentials of Microbiology. 1st ed., 299-300.
5. Boren, T. (2014). Helicobacter Pylori; Multitalented Adaptation of Binding Properties. Anticancer Research, 34(10):5840-5841.
6. Fan, L.; Li, R.; Li, H.; Zhang, J.; and Wang, L. (2018). genes in Helicobacter pylori isolated from gastric ulcer patients. 42(4):155-62.
7. Kusters, J. G.; Vliet, A. H.; and Kuipers, E. J. (2006). Pathogenesis of Helicobacter pylori infection. Clin. Microbiol. Rev., 19(3):449-490.
8. Wang, YK.; Kuo, FC.; Liu, CJ.; Wu, MC.; Shih, HY.; Wang, SSW.; Wu, JY.; Kuo, CH.; Huang, YK.; and Wu, DC. (2015). Diagnosis of
Helicobacter pylori infection: Current options and developments. World J. Gastroenterol., 21(40):11221-35.
9. Fernandez-Caso, B., Miqueleiz, A., Valdez, V. B., & Alarcon, T. (2022). Are molecular methods helpful for the diagnosis of Helicobacter pylori infection and for the prediction of its antimicrobial resistance?. Front. Microbiol, 13(August), 1-6.
10. Sulo, P; and Sipkova, B. (2021). DNA diagnostics for reliable and universal identification of Helicobacter pylori. World. J. Gastroenterol., 27(41):7100-7112.
11. Idowu, A.; Mzukwa, A.; Harrison, U.; Palamides, P.; Haas, R.; Mbao, M.; Mamdoo, R.; Bolon, J.; Jolaiya, T.; Smith, S.; Ally, R.; Clarke, A.; and Njom, H. (2019). Detection of Helicobacter pylori and its virulence genes (cagA, dupA, and vacA) among patients with gastroduodenal diseases in Chris Hani Baragwanath Academic Hospital, South Africa. Gastroenterol, 19(1):1-10.
12. El-Khlousy, M.; Rahman, E, A.; Mostafa, S.; Bassam, A.; Elgawad, W, A.; Elnasr, E, S.; Mohey, M.; and Ghaith, D, (2016). Study of the clinical relevance of Helicobacter pylori virulence genes to gastric diseases among Egyptian patients, Arab J. Gastroenterol., 17(2):90-94.
13. Wilkinson, D. J.; Dickins, B.; Robinson, K.; Winter J. (2022). Genomic diversity of Helicobacter pylori populations from different regions of the human stomach, Gut Microbes, 14(8):1-19.
14. Marques, A. T.; Vitor, J. M. B.; Santos, A.; Oleastro, M.; and Vale, F. F. (2020). Trends in Helicobacter pylori resistance to clarithromycin: From phenotypic to genomic approaches. Microbial Genomics, 6(3):1-11.
15. Vital, J. S.; Tanoeiro, L.; Lopes-Oliveira, R.; and Vale, F. F. (2022). Biomarker characterization and prediction of virulence and antibiotic
resistance from Helicobacter pylori next generation sequencing data. Biomolecules, 12(5):1-26.
16. Suzuki, R.; Satou, K.; Shiroma, A.; Shimoji, M.; Teruya, K.; Matsumoto, T.; Akada, J.; Hirano, T.; and Yamaoka, Y. (2019). Genome-wide mutation analysis of Helicobacter pylori after inoculation to Mongolian gerbils. Gut Pathogens, 11(1):4-9.
17. Vianna, J. S.; Ramis, I. B.; Ramos, D. F.; Gastal, O. L.; Azevedo, R.; Goncalves, C. V.; and Eduardo, P. (2018). The interplay between
mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori, Rev. Inst. Med. Trop. Sao Paulo, 60(25):1-5.
18. Jabar, S. A., & Alfaisal, A. H. M. (2017). The correlation between KRAS mutations and H. pylori in gastric cancer patients. Iraqi J.
Biotechnol., 16(3):82-93.
19. Dai, J., Zhao, J., Mao, L., Hu, Y. U. E., & Lv, B. I. N. (2022). Study on the value of antibiotic‑resistant gene detection in Helicobacter
pylori in China. Experiment. Therap. Med. 23(228):1-10.
20. Aziz, S.; Rasheed, F.; Akhter, T. S.; Zahra, R.; & Konig, S. (2022). Microbial Proteins in Stomach Biopsies Associated with Gastritis, Ulcer, and Gastric Cancer. Molecules, 27(17):1-13.
21. Jin, F.; and Yang, H. (2022). Complete Genome Sequence of Helicobacter pylori Strain 3192, Isolated from a Chinese Patient with Chronic Nonatrophic. Microbiol. Res. Announc., 11(7):0- 11.
22. Jia, X.; Huang, Q.; Lin, M.; and Chu, Y. (2022). Revealing the novel effect of Jinghua Weikang capsule against the antibiotic resistance of Helicobacter pylori. Front. Microbiol., 1-15.
23. Mohammed, H. A., & Obaid, Z. H. (2022). Pathogenesis of Helicobacter pylori: An Overview of Bacterial Virulence Agents and the
Mechanism of Occurrence Disease. J. Uni. Babyl. Pure Appli. Sci., 3(1):47-53.
24. Saber, F. omer, & Ali, M. K. (2022). Isolation and Identification of H. pylori among Iraq patients with chronic gastric inflammation. J. Faculty. Med. Baghdad, 64(2):102-108.
25. Hamed, A. T.; and Baqer, AA. B. (2022). Review on stimulations of the spread of Helicobacter pylori infection in Iraq. Inter. J. Appli. Sci. Technol., 3(6):146-156.
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Published
Mar 13, 2023
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How to Cite
Ahmed W. Al-Hilfi, Ihsan E. Alsaimary, & Ali D. Al-Hilfi. (2023). New recording genes and genetic mutations of H. pylori genome in gastroduodenal disease patients in Basrah province-Iraq. Journal of Population Therapeutics and Clinical Pharmacology, 30(3), 137-143. https://doi.org/10.47750/jptcp.2023.30.03.016
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How to Cite
Ahmed W. Al-Hilfi, Ihsan E. Alsaimary, & Ali D. Al-Hilfi. (2023). New recording genes and genetic mutations of H. pylori genome in gastroduodenal disease patients in Basrah province-Iraq. Journal of Population Therapeutics and Clinical Pharmacology, 30(3), 137-143. https://doi.org/10.47750/jptcp.2023.30.03.016